SRR2933759.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933759.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	592419
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGC	12079	2.0389285286258545	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCG	9828	1.6589609718796998	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTC	8584	1.4489744589555704	No Hit
GCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGC	6997	1.1810897354743854	No Hit
CCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGC	6829	1.1527314282627668	No Hit
CTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGCT	4572	0.7717510748304832	No Hit
TCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGC	4173	0.7044000952028885	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTAGGAATT	3826	0.6458266868550806	No Hit
GAACTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCT	2409	0.4066378694808911	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTAGGAATTCGTA	2266	0.38249954846147743	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTC	1994	0.33658609869028505	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCT	1993	0.33641729924259683	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1550	0.2616391439167211	No Hit
TGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGCTT	1541	0.26011994888752726	Illumina Single End Adapter 2 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTG	1420	0.2396952157172542	No Hit
TGCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTG	922	0.15563309076852702	No Hit
TCCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTG	916	0.15462029408239775	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCTAGGAATTCGT	820	0.13841554710432988	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATG	779	0.13149476974911337	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCC	776	0.13098837140604877	No Hit
TTCCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCT	731	0.12339239626007943	No Hit
TTCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTG	651	0.10988844044502286	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	30	2.1637134E-6	45.000004	1
TATGCGT	30	2.1637134E-6	45.000004	11
AACGTCG	20	7.030515E-4	45.0	20
TGATAGT	35	1.210592E-7	45.0	32
CGAACGG	20	7.030515E-4	45.0	11
TTAATCG	20	7.030515E-4	45.0	20
GCGAACG	20	7.030515E-4	45.0	10
ACGCACA	25	3.8885097E-5	45.0	31
CACGCAC	25	3.8885097E-5	45.0	30
AACGGCG	20	7.030515E-4	45.0	13
CTACGAA	25	3.8885097E-5	45.0	11
TCGTCCG	35	1.210592E-7	45.0	1
CACCGGA	20	7.030515E-4	45.0	16
GCCGTTG	25	3.8885097E-5	45.0	1
TAATGCG	20	7.030515E-4	45.0	16
ATCGCGG	20	7.030515E-4	45.0	2
TAATCGT	20	7.030515E-4	45.0	21
TCCTAGC	95	0.0	42.63158	15
TGTTCCG	60	3.6379788E-12	41.250004	1
GCGGGCT	180	0.0	41.250004	5

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content