SRR2933758.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933758.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	507621
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGC	17953	3.5366937144050383	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCG	15261	3.006376804742121	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTC	11936	2.351360562309282	No Hit
GCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC	8492	1.6729016333051627	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAATCACGT	5763	1.135295821094872	No Hit
CCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC	4800	0.9455873574970303	No Hit
CTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCT	4218	0.8309348904005153	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAATCACGTCGTA	3701	0.7290872521034394	No Hit
GAACTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCT	3533	0.6959916945910434	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCT	3102	0.6110858297824557	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTC	2983	0.5876431432111753	No Hit
TCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC	2553	0.502934275768733	No Hit
TGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCTT	1374	0.2706743810835249	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGAATCACGTCGT	1329	0.26180949960699024	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1248	0.24585271294922786	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATG	1126	0.22181903427951172	No Hit
CGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTG	1045	0.2058622476217493	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTAT	825	0.16252282706980206	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGAATCACG	781	0.15385494295941263	No Hit
TCCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTG	721	0.14203510099069977	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	663	0.13060925375427732	No Hit
TGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTG	662	0.1304122563881321	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	621	0.1223353643761783	No Hit
TTCCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCT	581	0.11445546973036971	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCC	539	0.1061815803522707	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACAACG	20	7.0295617E-4	45.0	37
ACCGGTC	20	7.0295617E-4	45.0	17
ACACGCA	20	7.0295617E-4	45.0	22
CAAGTCA	20	7.0295617E-4	45.0	14
CGTGCCA	20	7.0295617E-4	45.0	22
GGGTCGA	20	7.0295617E-4	45.0	7
GAGTACA	20	7.0295617E-4	45.0	9
CATCTAA	20	7.0295617E-4	45.0	44
TTCGTCG	35	1.2101373E-7	45.0	1
TGCGATT	25	3.8877173E-5	45.0	15
CGTAGAC	20	7.0295617E-4	45.0	31
TCTCGAG	25	3.8877173E-5	45.0	1
GCGATTA	20	7.0295617E-4	45.0	9
GCGATAA	25	3.8877173E-5	45.0	9
CTACCAT	20	7.0295617E-4	45.0	3
CTATTCC	40	6.8030204E-9	45.0	20
CAAGACT	20	7.0295617E-4	45.0	37
CATCCTA	25	3.8877173E-5	45.0	14
GCTAAAA	25	3.8877173E-5	45.0	33
CCCATGC	20	7.0295617E-4	45.0	13

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content