##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933758.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	507621
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.246715561412945	30.0	16.0	33.0	16.0	34.0
2	27.570342834516303	31.0	25.0	33.0	16.0	34.0
3	28.567618360942514	31.0	28.0	34.0	16.0	34.0
4	32.32459453017113	35.0	28.0	35.0	27.0	37.0
5	33.689912355871805	35.0	33.0	37.0	28.0	37.0
6	32.81080372955414	35.0	32.0	37.0	26.0	37.0
7	33.483833411147295	35.0	32.0	35.0	29.0	37.0
8	33.37559714826613	35.0	33.0	37.0	28.0	37.0
9	34.690231491604955	37.0	32.0	39.0	29.0	39.0
10	33.427728561269134	35.0	31.0	38.0	27.0	39.0
11	33.60180528386336	35.0	32.0	39.0	27.0	39.0
12	33.63713282153417	35.0	31.0	39.0	25.0	39.0
13	33.83308413166516	35.0	32.0	38.0	25.0	39.0
14	33.89697431745338	36.0	32.0	39.0	23.0	40.0
15	34.35218795124709	36.0	32.0	39.0	25.0	40.0
16	34.03854056471265	36.0	32.0	39.0	27.0	40.0
17	34.016951623356796	36.0	32.0	38.0	26.0	40.0
18	34.071947378063555	36.0	32.0	39.0	25.0	40.0
19	33.62071900098696	35.0	32.0	39.0	25.0	40.0
20	34.39443009646961	36.0	32.0	39.0	27.0	40.0
21	34.00330364583026	36.0	32.0	39.0	25.0	40.0
22	34.91847067004714	37.0	33.0	39.0	27.0	40.0
23	35.82587993798523	37.0	34.0	39.0	30.0	40.0
24	36.00746817015057	38.0	34.0	40.0	30.0	40.0
25	34.05556704706858	36.0	32.0	39.0	24.0	40.0
26	34.86891204264599	37.0	33.0	39.0	27.0	40.0
27	35.24116023568765	37.0	34.0	39.0	29.0	40.0
28	35.35245783763871	37.0	34.0	39.0	29.0	40.0
29	35.92499325284021	38.0	34.0	40.0	30.0	40.0
30	33.74307603507341	36.0	32.0	39.0	24.0	40.0
31	34.4413548690854	36.0	32.0	39.0	25.0	40.0
32	34.99500414679456	37.0	33.0	39.0	29.0	40.0
33	35.57562827384998	37.0	34.0	39.0	30.0	40.0
34	35.76459799732478	38.0	34.0	40.0	30.0	40.0
35	35.98587331887373	38.0	35.0	40.0	30.0	41.0
36	35.845772732018574	38.0	35.0	40.0	30.0	41.0
37	36.04332169078899	38.0	35.0	40.0	30.0	41.0
38	35.50209703696262	37.0	34.0	40.0	30.0	41.0
39	35.511688838720225	37.0	34.0	40.0	30.0	41.0
40	34.83337962771438	37.0	33.0	39.0	27.0	40.0
41	34.132301461129465	36.0	32.0	39.0	25.0	40.0
42	35.39886844712886	37.0	34.0	39.0	29.0	40.0
43	35.436975617635994	37.0	34.0	40.0	29.0	40.0
44	35.91707001877385	38.0	34.0	40.0	31.0	41.0
45	36.20175288256396	38.0	35.0	40.0	31.0	41.0
46	35.97828695030348	38.0	35.0	40.0	31.0	41.0
47	35.6220250935245	37.0	34.0	40.0	30.0	41.0
48	35.80614277187114	37.0	34.0	40.0	31.0	41.0
49	35.98128131026888	37.0	35.0	40.0	31.0	41.0
50	35.70576670389917	37.0	34.0	40.0	30.0	41.0
51	34.938651080235054	36.0	34.0	39.0	28.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	0.0
11	7.0
12	17.0
13	19.0
14	22.0
15	41.0
16	96.0
17	161.0
18	339.0
19	619.0
20	890.0
21	1234.0
22	1632.0
23	2090.0
24	2827.0
25	4126.0
26	5816.0
27	7667.0
28	10617.0
29	14843.0
30	20656.0
31	28862.0
32	39377.0
33	48667.0
34	55560.0
35	67983.0
36	78500.0
37	73355.0
38	37914.0
39	3682.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.09027010308873	3.9777708172041737	43.36975814633358	15.562200933373521
2	17.51148987138042	18.860921829475142	42.73621461681058	20.891373682333867
3	18.46988205767689	19.14282506042894	44.62266139501715	17.76463148687702
4	18.34006079338719	3.489414346530187	55.806398868447125	22.364125991635493
5	27.647004359551712	4.491342950744748	44.26195921760526	23.59969347209828
6	18.917460073558818	16.65691529704248	46.22129502128556	18.20432960811314
7	68.32893044219999	1.3718896578352746	24.592757194836302	5.706422705128433
8	66.07409858930187	12.489239018874319	12.210684743144984	9.225977648678837
9	62.64358645524909	3.3999775423002596	13.749234172739111	20.207201829711536
10	39.87620685511435	20.77199327844987	21.943733612281605	17.408066254154182
11	34.66188357061666	19.469249696131563	26.322985061689725	19.54588167156205
12	26.97504634363039	16.78279661400927	31.09071531713621	25.15144172522413
13	26.901369328692077	19.97238096926644	36.5930487509382	16.533200951103282
14	21.445133278568065	24.75724999556756	30.181572472376043	23.61604425348833
15	15.17904105622108	20.998146254784576	42.671599480714946	21.151213208279405
16	20.751505552370762	22.15136883619866	27.060543200537406	30.036582410893164
17	20.451872558463894	22.979348766106998	32.68324202505413	23.885536650374984
18	20.741064691965068	20.16878734331322	33.444833842571526	25.645314122150186
19	20.51786667612254	23.350688801290726	29.111285782109093	27.02015874047764
20	20.383908467143794	27.322549697510546	35.451252016760535	16.842289818585126
21	22.231743761585907	28.321326343866783	30.618512630486126	18.82841726406118
22	17.263470187403595	20.962292734146146	34.704041006971735	27.070196071478524
23	18.665106447526796	27.447249030280464	30.67583886403439	23.21180565815835
24	23.227171452717677	22.898776843353605	25.760754578711282	28.113297125217436
25	20.32855220725699	29.926263885851846	22.45848773001905	27.286696176872116
26	21.066307343470818	21.48118379657264	27.630456580795514	29.822052279161028
27	20.911664411046825	27.334960531577696	26.680732278609437	25.072642778766046
28	14.615628589045764	26.597402392730007	31.878113789618634	26.908855228605592
29	23.96886653625441	22.63086042539611	27.381845904720254	26.018427133629224
30	20.581102830655155	28.70409222628694	28.196036018998427	22.518768924059486
31	23.32291217266425	28.203521918911946	24.181623691691243	24.29194221673256
32	25.02556040825734	26.40552695810457	24.623685781321104	23.945226852316985
33	22.691732611534984	22.868242251601096	21.33225378776686	33.107771349097064
34	24.815955210678833	25.57597104926707	26.83852716889175	22.769546571162344
35	19.09239373469577	26.170706097659473	23.880217721489064	30.8566824461557
36	28.25927217353104	28.342405062044318	21.096053945758744	22.302268818665894
37	17.35054302323978	30.628559496159536	31.37202755599158	20.64886992460911
38	20.582875806950458	28.782103183280437	23.125520811786746	27.50950019798235
39	22.64957517517991	23.847122163976668	30.003486853380775	23.499815807462653
40	21.002283199473624	21.379336158275564	26.867682779081242	30.750697863169567
41	22.7362540162838	27.70649756412757	20.96327772097687	28.59397069861176
42	21.083840109057743	19.781884516204016	29.767484008738805	29.366791365999433
43	28.853613227191154	19.026202619670975	24.79921043455649	27.32097371858138
44	22.178357475360556	20.990660354871054	29.576002568845656	27.254979600922734
45	20.030298194913136	18.67810827369238	26.252657001975884	35.0389365294186
46	26.128154666572108	21.91851794941502	27.982884868829306	23.97044251518357
47	14.894379862141244	21.385049081893776	39.14002769782968	24.5805433581353
48	19.76494274271553	26.976031330461115	26.141944482202273	27.117081444621082
49	20.74185268142965	18.416101776719245	35.54620474724253	25.295840794608576
50	24.622700794490378	18.594778387812955	27.313881813400155	29.468639004296517
51	19.945392330104546	18.967891399291993	24.806696334470008	36.280019936133456
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	79.0
1	254.0
2	429.0
3	1296.0
4	2163.0
5	1438.5
6	714.0
7	726.5
8	739.0
9	767.0
10	795.0
11	839.0
12	883.0
13	874.0
14	865.0
15	817.5
16	770.0
17	788.5
18	807.0
19	778.0
20	749.0
21	919.5
22	1090.0
23	1174.5
24	1259.0
25	1294.5
26	1656.0
27	1982.0
28	2535.5
29	3089.0
30	3422.5
31	3756.0
32	4518.5
33	5281.0
34	5914.0
35	6547.0
36	7215.0
37	7883.0
38	9074.0
39	10265.0
40	11647.0
41	13029.0
42	15344.5
43	17660.0
44	22250.5
45	26841.0
46	64434.5
47	102028.0
48	87819.5
49	73611.0
50	71324.5
51	69038.0
52	54977.0
53	40916.0
54	35643.0
55	30370.0
56	26141.5
57	21913.0
58	19855.0
59	17797.0
60	15289.5
61	12782.0
62	11367.5
63	9953.0
64	8522.0
65	7091.0
66	5735.5
67	4380.0
68	3564.5
69	2749.0
70	2414.5
71	2080.0
72	1721.5
73	1363.0
74	1245.5
75	851.5
76	575.0
77	482.0
78	389.0
79	326.5
80	264.0
81	171.5
82	79.0
83	73.5
84	68.0
85	54.5
86	41.0
87	21.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	507621.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.526460263648225
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.70682098330848	21.27902500852207
2	12.415734258054812	7.580168369450524
3	5.215910808714454	4.776698821228652
4	2.5630222210194127	3.129599839391861
5	1.6350415635732796	2.4956015659916493
6	1.1500298850679256	2.106380495312038
7	0.8479450468879255	1.811933254570711
8	0.7062995617464719	1.7248660404708664
9	0.5992154716717474	1.6462734556815743
>10	4.912138835794265	26.409571460674503
>50	0.13957382086978184	2.892693142396868
>100	0.09196219038616467	5.071620374167211
>500	0.00521771292971147	1.0737348831265956
>1k	0.007826569394567207	6.17442354821472
>5k	0.0013044282324278676	2.8381403224494015
>10k+	0.001956642348641802	8.989269418350787
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGC	17953	3.5366937144050383	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCG	15261	3.006376804742121	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTC	11936	2.351360562309282	No Hit
GCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC	8492	1.6729016333051627	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAATCACGT	5763	1.135295821094872	No Hit
CCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC	4800	0.9455873574970303	No Hit
CTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCT	4218	0.8309348904005153	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAATCACGTCGTA	3701	0.7290872521034394	No Hit
GAACTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCT	3533	0.6959916945910434	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCT	3102	0.6110858297824557	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTC	2983	0.5876431432111753	No Hit
TCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC	2553	0.502934275768733	No Hit
TGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCTT	1374	0.2706743810835249	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGAATCACGTCGT	1329	0.26180949960699024	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1248	0.24585271294922786	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATG	1126	0.22181903427951172	No Hit
CGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTG	1045	0.2058622476217493	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTAT	825	0.16252282706980206	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGAATCACG	781	0.15385494295941263	No Hit
TCCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTG	721	0.14203510099069977	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	663	0.13060925375427732	No Hit
TGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTG	662	0.1304122563881321	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	621	0.1223353643761783	No Hit
TTCCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCT	581	0.11445546973036971	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCC	539	0.1061815803522707	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.9550432310720006	0.0
2	0.0	0.0	0.0	4.3481258655571775	0.0
3	0.0	0.0	0.0	5.361283319642016	0.0
4	0.0	0.0	0.0	7.3273170337712585	0.0
5	0.0	0.0	0.0	11.745573961676133	0.0
6	0.0	0.0	0.0	12.760701389422424	0.0
7	0.0	0.0	0.0	14.40129545467977	0.0
8	0.0	0.0	0.0	16.35807029260019	0.0
9	0.0	0.0	0.0	16.939803514827005	0.0
10	0.0	0.0	0.0	21.345649608664733	0.0
11	1.9699736614521465E-4	0.0	0.0	23.765368257026402	0.0
12	1.9699736614521465E-4	0.0	0.0	29.60653716059816	0.0
13	1.9699736614521465E-4	0.0	0.0	30.642152314423555	0.0
14	1.9699736614521465E-4	0.0	0.0	31.065893649001914	0.0
15	1.9699736614521465E-4	0.0	0.0	32.27841243762571	0.0
16	1.9699736614521465E-4	0.0	0.0	33.337076283290095	0.0
17	1.9699736614521465E-4	0.0	0.0	34.560626924418024	0.0
18	1.9699736614521465E-4	0.0	0.0	35.757582921116345	0.0
19	1.9699736614521465E-4	0.0	0.0	37.872743641417514	0.0
20	1.9699736614521465E-4	0.0	0.0	39.00252353626032	0.0
21	1.9699736614521465E-4	0.0	0.0	40.214648330151824	0.0
22	1.9699736614521465E-4	0.0	0.0	41.53453068332476	0.0
23	1.9699736614521465E-4	0.0	0.0	42.67790339643159	0.0
24	3.939947322904293E-4	0.0	0.0	43.61364088562136	0.0
25	3.939947322904293E-4	0.0	0.0	44.43177094722243	0.0
26	3.939947322904293E-4	0.0	0.0	45.15652425727068	0.0
27	3.939947322904293E-4	0.0	0.0	46.02981358139242	0.0
28	3.939947322904293E-4	0.0	0.0	46.821742993296176	0.0
29	3.939947322904293E-4	0.0	0.0	47.72793087756417	0.0
30	3.939947322904293E-4	0.0	0.0	48.74069433691672	0.0
31	3.939947322904293E-4	0.0	0.0	49.43826201043692	0.0
32	3.939947322904293E-4	0.0	0.0	50.13188973663422	0.0
33	3.939947322904293E-4	0.0	0.0	50.87259983334023	0.0
34	3.939947322904293E-4	0.0	0.0	51.63281266929461	0.0
35	3.939947322904293E-4	0.0	0.0	52.52383175636942	0.0
36	3.939947322904293E-4	0.0	0.0	53.23203728766146	0.0
37	3.939947322904293E-4	0.0	0.0	53.963094513426356	0.0
38	3.939947322904293E-4	0.0	0.0	54.80604624316173	0.0
39	3.939947322904293E-4	0.0	0.0	56.37158431191775	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACAACG	20	7.0295617E-4	45.0	37
ACCGGTC	20	7.0295617E-4	45.0	17
ACACGCA	20	7.0295617E-4	45.0	22
CAAGTCA	20	7.0295617E-4	45.0	14
CGTGCCA	20	7.0295617E-4	45.0	22
GGGTCGA	20	7.0295617E-4	45.0	7
GAGTACA	20	7.0295617E-4	45.0	9
CATCTAA	20	7.0295617E-4	45.0	44
TTCGTCG	35	1.2101373E-7	45.0	1
TGCGATT	25	3.8877173E-5	45.0	15
CGTAGAC	20	7.0295617E-4	45.0	31
TCTCGAG	25	3.8877173E-5	45.0	1
GCGATTA	20	7.0295617E-4	45.0	9
GCGATAA	25	3.8877173E-5	45.0	9
CTACCAT	20	7.0295617E-4	45.0	3
CTATTCC	40	6.8030204E-9	45.0	20
CAAGACT	20	7.0295617E-4	45.0	37
CATCCTA	25	3.8877173E-5	45.0	14
GCTAAAA	25	3.8877173E-5	45.0	33
CCCATGC	20	7.0295617E-4	45.0	13
>>END_MODULE