SRR2933756.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933756.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	478128
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGC	11092	2.3198808687213464	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCG	8284	1.7325904360338655	No Hit
GAATCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTC	7462	1.5606699461232139	No Hit
GCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC	5599	1.171025332128635	TruSeq Adapter, Index 15 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC	4892	1.0231569788843156	TruSeq Adapter, Index 15 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACCTCGTTT	3521	0.7364136800187397	No Hit
TCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC	3274	0.6847538734397484	TruSeq Adapter, Index 15 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGCT	3170	0.6630023759328046	TruSeq Adapter, Index 14 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCT	2405	0.5030033798480741	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTA	2308	0.48271592544255937	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTC	1848	0.3865073787772312	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCT	1782	0.37270354382090154	No Hit
TGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGCTT	1333	0.2787956363149617	TruSeq Adapter, Index 15 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1000	0.20914901448984372	No Hit
CGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG	911	0.19053475220024763	TruSeq Adapter, Index 14 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG	838	0.17526687414248904	TruSeq Adapter, Index 14 (95% over 21bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCACCTCGTTTCGT	792	0.16564601947595622	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATG	682	0.14263962788207343	No Hit
TTCCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCT	666	0.1392932436502359	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCC	570	0.11921493825921092	No Hit
TGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG	540	0.11294046782451561	TruSeq Adapter, Index 14 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTC	511	0.10687514640431015	No Hit
TTCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG	504	0.10541110330288123	TruSeq Adapter, Index 14 (95% over 21bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTAT	499	0.10436535823043203	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGT	20	7.029149E-4	45.0	19
CATATCG	20	7.029149E-4	45.0	17
GGTAATC	35	1.2099372E-7	45.0	8
CTCGTAC	20	7.029149E-4	45.0	29
AACCCGA	20	7.029149E-4	45.0	24
AAGCGGT	20	7.029149E-4	45.0	11
TAACGGC	20	7.029149E-4	45.0	14
TCGATGT	25	3.8873754E-5	45.0	43
ACGGGGT	25	3.8873754E-5	45.0	5
CGTTAGG	25	3.8873754E-5	45.0	2
TGGTTCG	25	3.8873754E-5	45.0	1
AAACGAT	20	7.029149E-4	45.0	43
AACATAG	20	7.029149E-4	45.0	11
CGGGCCC	20	7.029149E-4	45.0	6
GCGATCG	25	3.8873754E-5	45.0	9
CGTACGG	20	7.029149E-4	45.0	31
TCGTACG	20	7.029149E-4	45.0	30
CGACTCC	20	7.029149E-4	45.0	28
GATTCGG	20	7.029149E-4	45.0	9
TGAAACG	30	2.162833E-6	44.999996	33

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content