SRR2933755.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933755.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	335689
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGC	10212	3.0421014689191486	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCG	7452	2.219911882724784	No Hit
GAATCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTC	6983	2.0801992320272635	No Hit
GCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC	3573	1.0643780403885739	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCATTAGACGT	2919	0.8695548558338224	No Hit
CCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC	2173	0.6473253517392586	No Hit
GAACTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCT	2127	0.6336221919693525	No Hit
CTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGCT	1896	0.5648084983422156	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCATTAGACGTCGTA	1825	0.5436579691321431	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTC	1580	0.47067374861851297	No Hit
TCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC	1494	0.44505479774434076	No Hit
GAATGACTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCT	1445	0.4304579536416147	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCATTAGACGTCGT	696	0.20733476521423103	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	677	0.20167476443970458	No Hit
TGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGCTT	608	0.18112002478484548	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATG	605	0.18022634045202554	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCATTAGACGTCGTAT	430	0.12809475437086112	No Hit
CGCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTG	387	0.115285278933775	No Hit
TCCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTG	359	0.10694422516078869	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGGG	25	3.884884E-5	45.000004	3
CTTGTCG	25	3.884884E-5	45.000004	1
GTTCTCC	25	3.884884E-5	45.000004	16
CCTACTT	25	3.884884E-5	45.000004	12
GTTCGCG	25	3.884884E-5	45.000004	1
CCTCAAG	25	3.884884E-5	45.000004	24
GGACGTT	25	3.884884E-5	45.000004	8
CGGGACG	25	3.884884E-5	45.000004	6
ATGCCTA	25	3.884884E-5	45.000004	10
ATGGGCG	35	1.2085002E-7	45.0	5
GAGTGCC	20	7.0261426E-4	45.0	9
TCGTTGA	20	7.0261426E-4	45.0	24
GTACCAT	20	7.0261426E-4	45.0	9
TTAATCG	20	7.0261426E-4	45.0	20
GTCGAGT	20	7.0261426E-4	45.0	14
CTCAACG	35	1.2085002E-7	45.0	16
TAGACCA	20	7.0261426E-4	45.0	35
GAACTTC	20	7.0261426E-4	45.0	9
GGGTATC	20	7.0261426E-4	45.0	7
CGCATGG	20	7.0261426E-4	45.0	2

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content