##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933755.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	335689
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.898635344023784	31.0	16.0	33.0	16.0	34.0
2	28.136114081784033	31.0	25.0	33.0	16.0	34.0
3	29.123948059066578	31.0	28.0	34.0	16.0	34.0
4	32.63997032968015	35.0	32.0	37.0	28.0	37.0
5	33.784738254753655	35.0	33.0	37.0	28.0	37.0
6	33.043671374397135	35.0	33.0	37.0	28.0	37.0
7	33.43793809150732	35.0	32.0	35.0	29.0	37.0
8	33.57902105818183	35.0	35.0	37.0	28.0	37.0
9	34.68628700970243	37.0	33.0	39.0	28.0	39.0
10	33.54090542138706	35.0	31.0	39.0	27.0	39.0
11	33.76235742011207	35.0	32.0	39.0	27.0	39.0
12	33.9939378412757	37.0	32.0	39.0	26.0	39.0
13	34.36364313397222	35.0	33.0	39.0	27.0	39.0
14	34.27703618527864	37.0	32.0	39.0	25.0	40.0
15	34.80424738373912	37.0	32.0	39.0	27.0	40.0
16	34.545141485124624	36.0	32.0	39.0	27.0	40.0
17	34.44049700764696	36.0	32.0	38.0	27.0	40.0
18	34.329215434524215	36.0	32.0	39.0	27.0	40.0
19	34.168602486229815	36.0	32.0	39.0	25.0	40.0
20	35.06380310346779	37.0	33.0	39.0	27.0	40.0
21	34.42689811104921	37.0	32.0	39.0	25.0	40.0
22	35.42825055333955	37.0	34.0	39.0	28.0	40.0
23	36.208603796966834	38.0	34.0	40.0	30.0	40.0
24	36.415256383140346	38.0	35.0	40.0	31.0	41.0
25	34.30715632624244	37.0	32.0	39.0	25.0	40.0
26	35.032807151857824	37.0	33.0	39.0	27.0	40.0
27	35.60096100855256	37.0	34.0	39.0	30.0	40.0
28	35.78490805477689	38.0	34.0	40.0	30.0	40.0
29	36.24700243380033	38.0	35.0	40.0	30.0	41.0
30	34.16233775905675	37.0	33.0	39.0	24.0	40.0
31	34.949625993106714	37.0	33.0	39.0	27.0	40.0
32	35.33281698238548	37.0	34.0	39.0	29.0	40.0
33	36.05332614413937	38.0	35.0	40.0	30.0	40.0
34	36.205073743852196	38.0	35.0	40.0	30.0	41.0
35	36.423788089571005	38.0	35.0	40.0	31.0	41.0
36	36.343043710100716	38.0	35.0	40.0	30.0	41.0
37	36.58376354304133	38.0	35.0	40.0	31.0	41.0
38	36.17674692944958	38.0	35.0	40.0	30.0	41.0
39	36.097313287000766	38.0	35.0	40.0	30.0	41.0
40	35.667141312345656	38.0	34.0	40.0	30.0	41.0
41	35.11829103723983	37.0	33.0	40.0	28.0	41.0
42	36.041779742559335	38.0	35.0	40.0	30.0	41.0
43	36.028761740777924	38.0	35.0	40.0	31.0	41.0
44	36.505387427053016	38.0	35.0	40.0	31.0	41.0
45	36.773918716430984	39.0	35.0	40.0	32.0	41.0
46	36.515724375835966	38.0	35.0	40.0	31.0	41.0
47	36.28032494362341	38.0	35.0	40.0	31.0	41.0
48	36.43215893282175	38.0	35.0	40.0	31.0	41.0
49	36.623723148509484	38.0	35.0	40.0	32.0	41.0
50	36.37745353586206	38.0	35.0	40.0	31.0	41.0
51	35.76606621009327	37.0	34.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	5.0
10	9.0
11	15.0
12	15.0
13	10.0
14	20.0
15	27.0
16	57.0
17	110.0
18	266.0
19	448.0
20	619.0
21	744.0
22	1003.0
23	1221.0
24	1659.0
25	2293.0
26	3059.0
27	4137.0
28	5669.0
29	7945.0
30	10919.0
31	15633.0
32	21547.0
33	27898.0
34	34201.0
35	44072.0
36	55256.0
37	57895.0
38	35097.0
39	3839.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.90009502843406	3.896463691095031	46.35064002692969	16.852801253541223
2	16.77862545391717	16.250755907998176	46.892808522173794	20.077810115910857
3	17.54034240025738	16.133385365621155	46.77067166335507	19.555600570766394
4	17.615709778991864	4.1023089824212295	55.66193709058086	22.62004414800604
5	25.750620365874365	4.800276446353619	45.49806517341944	23.951038014352573
6	19.652714268266163	15.052027322909002	47.53655913658177	17.75869927224306
7	73.46412900035449	1.5550107391067327	20.00899642228372	4.971863838255051
8	71.75689402989076	10.843667799659803	9.89338345909458	7.506054711354855
9	67.05492286014734	4.7007795906329966	11.219313114221794	17.02498443499787
10	37.36643142909062	27.786135381260628	19.120376300683073	15.727056888965679
11	32.72195395142528	22.48301255030698	25.175385550315916	19.619647947951822
12	26.611238378379987	17.87338876162162	30.355179943340417	25.16019291665798
13	24.272168584612544	21.624181906466998	35.81469753253756	18.288951976382904
14	21.30245554665181	26.92879421130868	27.83975644122983	23.92899380080968
15	15.637688455683682	22.993902093902392	39.42339486846456	21.945014581949362
16	17.84210980997292	23.1508926417011	29.898507249269414	29.108490299056566
17	18.903806797363035	24.355579122342405	31.970663322301295	24.76995075799326
18	17.837343493531215	22.52590939828234	32.73148658430869	26.905260523877754
19	19.8201311332811	25.78696352874238	29.52733035637152	24.865574981605
20	22.623618885337322	24.88940656381353	35.13371007092875	17.3532644799204
21	22.478246233865274	28.770379726473017	28.136757534503666	20.614616505158047
22	19.407844761073495	22.570891509700942	31.267035857594383	26.75422787163118
23	18.963683647661973	28.945541855705727	28.776635516802756	23.314138979829544
24	22.533654662500112	22.275379890315143	27.739663795953994	27.451301651230754
25	17.88053823628418	30.46331574761163	24.759524440777028	26.896621575327163
26	18.816523627524287	25.338036098889148	29.10819240427896	26.737247869307602
27	20.200840659062404	27.08489107477457	28.473974422754395	24.24029384340863
28	15.935881128067944	25.762238262201027	35.04732058542282	23.254560024308212
29	20.14870907298124	26.78044261206057	29.072742925743768	23.998105389214423
30	20.825228112925952	26.32734465532085	32.35464969063627	20.492777541116926
31	22.342704110054246	27.44266270268016	26.566256266961386	23.64837692030421
32	23.128848428158204	31.621828537723907	23.22387686221473	22.02544617190316
33	24.59657599742619	23.30132950439246	25.030608688399088	27.07148580978227
34	21.94441879239415	29.025675550881918	26.595449955166835	22.434455701557095
35	19.85855955959236	23.728808510258006	27.78702906559345	28.625602864556182
36	23.225068441325156	28.068837525209346	27.750983797503046	20.955110235962454
37	17.855217180187612	27.803413278361816	34.10716466729622	20.23420487415435
38	23.032628414991258	25.38450768419579	27.655359573891307	23.927504326921646
39	22.907512608396463	30.425483110855584	26.031237246379835	20.635767034368122
40	24.6776033769352	23.615012705212266	27.381296378493186	24.32608753935935
41	21.760617714610845	27.72953537351537	25.24807187605194	25.26177503582185
42	21.051032354351797	22.119580921626863	28.84544921042989	27.98393751359145
43	26.697032074330707	22.975134722913175	25.81198669006134	24.515846512694786
44	21.272666068891148	22.394835696135413	31.01740003396	25.315098201013438
45	20.144836440872353	21.635799802793656	28.186505962363974	30.032857793970013
46	25.52273086100528	25.683594040912865	27.431342701131108	21.362332396950748
47	17.900795081161434	23.04305473220749	36.68127344059531	22.374876746035767
48	19.939289044323765	25.268328720929194	28.41886388889716	26.373518345849877
49	20.078703800243677	20.68313230400758	35.1048142775009	24.13334961824784
50	24.085686453830778	20.06678800913941	29.326847171042242	26.520678365987564
51	19.729571120888682	20.790076529168367	26.324067812767176	33.15628453717578
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	29.0
1	173.5
2	318.0
3	826.5
4	1335.0
5	947.5
6	560.0
7	585.5
8	611.0
9	628.5
10	646.0
11	656.5
12	667.0
13	664.5
14	662.0
15	672.5
16	683.0
17	627.0
18	571.0
19	608.0
20	645.0
21	738.0
22	831.0
23	914.5
24	998.0
25	1101.5
26	1649.5
27	2094.0
28	2385.0
29	2676.0
30	3473.5
31	4271.0
32	4648.5
33	5026.0
34	6125.0
35	7224.0
36	7786.0
37	8348.0
38	9804.5
39	11261.0
40	12635.5
41	14010.0
42	16914.0
43	19818.0
44	40528.0
45	61238.0
46	51894.5
47	42551.0
48	40036.0
49	37521.0
50	30103.5
51	22686.0
52	20226.5
53	17767.0
54	16268.0
55	14769.0
56	13611.0
57	12453.0
58	11400.0
59	10347.0
60	9546.5
61	8746.0
62	7976.5
63	7207.0
64	6132.0
65	5057.0
66	4388.0
67	3719.0
68	2887.5
69	2056.0
70	1829.0
71	1602.0
72	1407.5
73	1213.0
74	1139.0
75	834.5
76	604.0
77	480.5
78	357.0
79	229.0
80	101.0
81	98.5
82	96.0
83	55.5
84	15.0
85	19.5
86	24.0
87	14.5
88	5.0
89	3.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	335689.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.929726109859452
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.43497340519257	21.380291886825077
2	12.048995514232033	7.212462712797797
3	4.570035157689931	4.10339701746264
4	2.2619995236339547	2.7080410481198727
5	1.3849994888623518	2.072632768197276
6	0.8389996877000097	1.506661851547134
7	0.6559997557761228	1.374372511295983
8	0.5579997922600755	1.3360624761362025
9	0.4079998481041308	1.0990191335958803
>10	5.590997918505377	37.102071861949796
>50	0.172999935593173	3.3856493570905757
>100	0.05799997840695973	2.872354745402957
>500	0.00399999851082481	0.7739824290520003
>1k	0.008999996649355822	5.696223352559037
>5k	0.001999999255412405	4.320354355516483
>10k+	9.999996277062025E-4	3.056422492451287
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGC	10212	3.0421014689191486	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCG	7452	2.219911882724784	No Hit
GAATCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTC	6983	2.0801992320272635	No Hit
GCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC	3573	1.0643780403885739	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCATTAGACGT	2919	0.8695548558338224	No Hit
CCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC	2173	0.6473253517392586	No Hit
GAACTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCT	2127	0.6336221919693525	No Hit
CTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGCT	1896	0.5648084983422156	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCATTAGACGTCGTA	1825	0.5436579691321431	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTC	1580	0.47067374861851297	No Hit
TCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC	1494	0.44505479774434076	No Hit
GAATGACTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCT	1445	0.4304579536416147	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCATTAGACGTCGT	696	0.20733476521423103	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	677	0.20167476443970458	No Hit
TGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGCTT	608	0.18112002478484548	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATG	605	0.18022634045202554	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCATTAGACGTCGTAT	430	0.12809475437086112	No Hit
CGCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTG	387	0.115285278933775	No Hit
TCCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTG	359	0.10694422516078869	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6741358817238575	0.0
2	0.0	0.0	0.0	3.0206530449314695	0.0
3	0.0	0.0	0.0	3.704023664761133	0.0
4	0.0	0.0	0.0	5.282568091298791	0.0
5	0.0	0.0	0.0	8.796236993169273	0.0
6	0.0	0.0	0.0	9.593105523267072	0.0
7	0.0	0.0	0.0	10.70604041240553	0.0
8	0.0	0.0	0.0	12.31824694881274	0.0
9	0.0	0.0	0.0	12.802921751978767	0.0
10	0.0	0.0	0.0	16.221860114570333	0.0
11	0.0	0.0	0.0	18.097405634381822	0.0
12	0.0	0.0	0.0	23.096675792176686	0.0
13	0.0	0.0	0.0	23.974869596561103	0.0
14	0.0	0.0	0.0	24.302553851928423	0.0
15	0.0	0.0	0.0	25.20904766018547	0.0
16	0.0	0.0	0.0	26.10660462511432	0.0
17	0.0	0.0	0.0	27.093827918102768	0.0
18	0.0	0.0	0.0	28.23387123200343	0.0
19	0.0	0.0	0.0	29.898507249269414	0.0
20	0.0	0.0	0.0	30.78176526487314	0.0
21	0.0	0.0	0.0	31.810395931948918	0.0
22	0.0	0.0	0.0	32.956099246624106	0.0
23	0.0	0.0	0.0	33.90876674541018	0.0
24	0.0	0.0	0.0	34.63980052965692	0.0
25	0.0	0.0	0.0	35.329724834593925	0.0
26	0.0	0.0	0.0	35.912704914370146	0.0
27	0.0	0.0	0.0	36.5016428896985	0.0
28	0.0	0.0	0.0	37.11768928978906	0.0
29	0.0	0.0	0.0	37.74863042875995	0.0
30	0.0	0.0	0.0	38.39297683272314	0.0
31	0.0	0.0	0.0	39.059665345006835	0.0
32	0.0	0.0	0.0	39.6354959501205	0.0
33	0.0	0.0	0.0	40.27299077419874	0.0
34	0.0	0.0	0.0	40.82826663965754	0.0
35	0.0	0.0	0.0	41.48720988772346	0.0
36	0.0	0.0	0.0	42.06810470405643	0.0
37	0.0	0.0	0.0	42.612656357521395	0.0
38	0.0	0.0	0.0	43.21053117617795	0.0
39	0.0	0.0	0.0	43.84266389425927	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGGG	25	3.884884E-5	45.000004	3
CTTGTCG	25	3.884884E-5	45.000004	1
GTTCTCC	25	3.884884E-5	45.000004	16
CCTACTT	25	3.884884E-5	45.000004	12
GTTCGCG	25	3.884884E-5	45.000004	1
CCTCAAG	25	3.884884E-5	45.000004	24
GGACGTT	25	3.884884E-5	45.000004	8
CGGGACG	25	3.884884E-5	45.000004	6
ATGCCTA	25	3.884884E-5	45.000004	10
ATGGGCG	35	1.2085002E-7	45.0	5
GAGTGCC	20	7.0261426E-4	45.0	9
TCGTTGA	20	7.0261426E-4	45.0	24
GTACCAT	20	7.0261426E-4	45.0	9
TTAATCG	20	7.0261426E-4	45.0	20
GTCGAGT	20	7.0261426E-4	45.0	14
CTCAACG	35	1.2085002E-7	45.0	16
TAGACCA	20	7.0261426E-4	45.0	35
GAACTTC	20	7.0261426E-4	45.0	9
GGGTATC	20	7.0261426E-4	45.0	7
CGCATGG	20	7.0261426E-4	45.0	2
>>END_MODULE