##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933754.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	666923
Sequences flagged as poor quality	0
Sequence length	51
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.36594629365009	33.0	31.0	34.0	31.0	34.0
2	32.57578760966408	34.0	31.0	34.0	31.0	34.0
3	32.60660226143048	34.0	31.0	34.0	31.0	34.0
4	36.1330933256163	37.0	35.0	37.0	35.0	37.0
5	36.16152989175662	37.0	35.0	37.0	35.0	37.0
6	36.14436599127635	37.0	36.0	37.0	35.0	37.0
7	36.21931317408456	37.0	37.0	37.0	35.0	37.0
8	36.11792665720031	37.0	37.0	37.0	35.0	37.0
9	37.93777242650201	39.0	38.0	39.0	35.0	39.0
10	37.423206277186424	39.0	37.0	39.0	34.0	39.0
11	37.388392663021065	39.0	37.0	39.0	34.0	39.0
12	37.38880350505231	39.0	37.0	39.0	34.0	39.0
13	37.38644641135483	39.0	37.0	39.0	34.0	39.0
14	38.60912729055678	40.0	38.0	41.0	34.0	41.0
15	38.648541135933236	40.0	38.0	41.0	34.0	41.0
16	38.69430953798265	40.0	38.0	41.0	34.0	41.0
17	38.63388127265067	40.0	38.0	41.0	34.0	41.0
18	38.17244719405389	39.0	38.0	40.0	34.0	41.0
19	37.68982326295539	38.0	37.0	40.0	34.0	41.0
20	37.1179641427871	38.0	35.0	40.0	34.0	41.0
21	36.98768373560366	38.0	35.0	40.0	33.0	41.0
22	37.014025607154046	38.0	35.0	40.0	33.0	41.0
23	36.93389491740426	38.0	35.0	40.0	33.0	41.0
24	36.828420972136215	38.0	35.0	40.0	33.0	41.0
25	36.748723615769734	38.0	35.0	40.0	33.0	41.0
26	36.69648670086352	38.0	35.0	40.0	33.0	41.0
27	36.60541471804091	38.0	35.0	40.0	33.0	41.0
28	36.441973061357906	37.0	35.0	40.0	33.0	41.0
29	36.28496093252144	37.0	35.0	40.0	32.0	41.0
30	36.070024575550704	37.0	35.0	40.0	31.0	41.0
31	35.626667546328434	37.0	35.0	40.0	30.0	41.0
32	34.94833136658955	37.0	35.0	40.0	24.0	41.0
33	34.12533380915038	37.0	34.0	40.0	18.0	41.0
34	33.41776786825466	37.0	33.0	40.0	15.0	41.0
35	32.925962967239094	37.0	33.0	40.0	10.0	41.0
36	32.61716569978843	37.0	32.0	40.0	10.0	41.0
37	32.388004312341906	37.0	31.0	40.0	9.0	41.0
38	32.27159507169493	37.0	31.0	40.0	8.0	41.0
39	32.185270263583654	37.0	31.0	40.0	8.0	41.0
40	32.05076448105704	37.0	31.0	40.0	8.0	41.0
41	31.874868613018293	36.0	30.0	40.0	8.0	41.0
42	31.706993161129546	36.0	30.0	40.0	8.0	41.0
43	31.519997061129995	36.0	29.0	40.0	8.0	41.0
44	31.36191884220517	36.0	28.0	40.0	7.0	41.0
45	31.346723684743218	35.0	28.0	40.0	7.0	41.0
46	31.16007395156562	35.0	27.0	40.0	7.0	41.0
47	31.018774281288845	35.0	26.0	40.0	7.0	41.0
48	30.934947512681376	35.0	26.0	40.0	7.0	41.0
49	30.92560760387631	35.0	27.0	40.0	7.0	41.0
50	30.830724086588706	35.0	27.0	40.0	7.0	41.0
51	29.975431946416602	35.0	24.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	1.0
11	2.0
12	5.0
13	7.0
14	12.0
15	24.0
16	48.0
17	131.0
18	254.0
19	494.0
20	978.0
21	1554.0
22	2602.0
23	4361.0
24	8348.0
25	16867.0
26	26706.0
27	29259.0
28	25037.0
29	20995.0
30	18560.0
31	18380.0
32	19660.0
33	23897.0
34	34550.0
35	47405.0
36	61685.0
37	68239.0
38	114306.0
39	122511.0
40	41.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.972355129452726	3.766101933806451	37.66911622481156	39.592426711929264
2	43.615679771128	3.7629531445159334	38.99190761152337	13.629459472832695
3	16.30922910141051	4.189089295166009	64.96042271746514	14.54125888595835
4	13.000751211159308	4.076332650096038	65.20272955048783	17.720186588256816
5	12.691120264258393	4.828893290529791	65.3372278358971	17.14275860931472
6	15.259332786543576	5.316205918824212	66.6859592486689	12.738502045963326
7	60.89488591636516	1.6956980041174168	34.221941663430414	3.1874744160870145
8	62.21257926327327	1.8601847589601799	32.699277127944306	3.2279588498222433
9	57.33675401808005	4.5489509283680425	34.3267513641005	3.7875436894514056
10	23.717580590263044	25.58151390790241	39.435287132097706	11.265618369736837
11	19.561628553821055	18.627337788620277	46.57329256900722	15.237741088551452
12	16.18282770274829	18.10178986179814	49.75611877233204	15.95926366312153
13	17.17529609865007	17.95739538147582	51.14263565659004	13.724672863284068
14	15.693715766287863	19.240901873229742	51.13064026881664	13.934742091665756
15	13.32102806470912	18.718352793350956	52.12685722339761	15.833761918542322
16	15.18781028694467	19.81173238889647	49.55294689192006	15.447510432238804
17	14.81325430372022	19.659840791215778	49.215876495487485	16.311028409576515
18	16.160186408326	17.7645695230184	50.08149366568554	15.993750402970058
19	15.88804104821696	19.921190302328753	49.08902526978377	15.101743379670518
20	16.74271242707179	21.991144404976286	47.57130883175419	13.694834336197731
21	15.475399708811963	23.31318607995226	47.918425365446986	13.292988845788795
22	15.102643033753521	20.202632087962176	48.33796405282169	16.35676082546261
23	13.298836597328329	22.713266748935034	47.179059651563975	16.808837002172663
24	13.239909254891494	22.072563099488246	47.9673065706236	16.72022107499666
25	14.053646372969594	23.65445486210552	45.55263501183795	16.739263753086938
26	14.41515737199047	22.705469746882322	46.12676425914236	16.752608621984848
27	14.405711004118915	22.02413172135314	47.64687977472662	15.923277499801328
28	12.674026836681296	22.19716518998445	47.698160057457905	17.430647915876346
29	15.022873705060405	21.111132769450148	46.776614391766365	17.089379133723085
30	17.315042366210193	20.672851288679503	47.212796679676664	14.799309665433642
31	17.52556142163338	22.998607035594816	43.999982006918344	15.475849535853465
32	16.615861201368073	23.40105229539242	43.42585275961393	16.557233743625577
33	17.034500234659774	23.356819302978003	41.49219625054167	18.116484211820556
34	17.131512933277154	23.76781127656416	41.558020940948204	17.54265484921048
35	17.988883274381003	25.411029459172944	38.8514116322274	17.748675634218642
36	18.889287069121924	26.162690445523694	38.69172303249401	16.256299452860375
37	18.163716051178323	26.252206026782705	38.85980840366879	16.724269518370185
38	17.670405728997203	28.10594326481468	37.353487584023945	16.87016342216418
39	19.316172931507836	26.138849612324062	36.67514840543811	17.869829050729994
40	21.751236649508265	24.852644158321123	36.41065010503461	16.985469087135996
41	20.42439681942293	25.10934545667191	34.393025881548546	20.073231842356616
42	21.29481214473035	25.071709927532865	35.58356811805861	18.049909809678176
43	19.9057462405705	25.17621974350862	37.12137683060863	17.79665718531225
44	19.073116386749295	25.738053718345295	36.63031564363502	18.558514251270385
45	20.411201892872192	24.411813657648633	35.75735129842575	19.41963315105342
46	19.972920412101548	24.85819202516632	36.5196581914254	18.649229371306735
47	17.61312775237921	27.21783474254149	37.648874007943945	17.520163497135353
48	17.660509534084145	26.533197985374624	37.90002743944953	17.9062650410917
49	20.133958492959458	24.14791512663381	37.76687863516478	17.951247745241954
50	18.053958253051704	24.32229807638963	36.86482547460501	20.758918195953655
51	17.275757471252305	23.498514821051305	35.669934910027095	23.555792797669294
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	792.0
1	1798.0
2	2804.0
3	33364.5
4	63925.0
5	39847.0
6	15769.0
7	15123.0
8	14477.0
9	15056.5
10	15636.0
11	15438.0
12	15240.0
13	14804.0
14	14368.0
15	13551.5
16	12735.0
17	11834.0
18	10933.0
19	10255.0
20	9577.0
21	9073.0
22	8569.0
23	8406.0
24	8243.0
25	8141.0
26	8214.5
27	8390.0
28	9030.0
29	9670.0
30	10201.0
31	10732.0
32	12125.0
33	13518.0
34	14219.5
35	14921.0
36	16122.0
37	17323.0
38	18248.0
39	19173.0
40	21801.0
41	24429.0
42	27523.5
43	30618.0
44	32992.5
45	35367.0
46	40414.5
47	45462.0
48	49093.0
49	52724.0
50	51526.0
51	50328.0
52	44236.5
53	38145.0
54	32471.0
55	26797.0
56	23408.0
57	20019.0
58	17482.0
59	14945.0
60	12975.0
61	11005.0
62	9402.0
63	7799.0
64	6584.0
65	5369.0
66	4245.0
67	3121.0
68	2470.5
69	1820.0
70	1627.0
71	1434.0
72	1131.0
73	828.0
74	867.5
75	738.5
76	570.0
77	423.5
78	277.0
79	170.0
80	63.0
81	46.0
82	29.0
83	26.0
84	23.0
85	14.5
86	6.0
87	4.0
88	2.0
89	2.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	666923.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.867840461922505
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.88533161479837	33.289256206780586
2	9.081113711708708	7.96737695043629
3	4.205485551501703	5.534567077145906
4	2.6550057456462253	4.658774739019847
5	1.7677122179395628	3.8772858779581956
6	1.2512226485798927	3.2933061318148114
7	0.9314171260310535	2.8601480521762834
8	0.7475059425787809	2.623317714670797
9	0.5684499062704135	2.244300281897877
>10	2.7614881731417893	19.356128964826066
>50	0.0991236140863681	2.9904575620513687
>100	0.04238388679195187	3.292591933375752
>500	0.0027344443091580966	0.8185372450611542
>1k	6.836110772895242E-4	0.7870492763007876
>5k	0.0	0.0
>10k+	3.418055386447621E-4	6.40690198648435
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	42729	6.406886552120709	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCC	3454	0.5179008671165937	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGC	1795	0.2691465131656878	No Hit
CCTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTTCTGC	952	0.1427451145034734	No Hit
CTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTTCTGCT	751	0.11260670272280307	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCG	710	0.106459066488935	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	691	0.10361016189275223	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	690	0.10346021954558472	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.9988469433502816E-4	0.0	0.0	0.12160324355285393	0.0
2	2.9988469433502816E-4	0.0	0.0	0.4421799817969991	0.0
3	2.9988469433502816E-4	0.0	0.0	0.6994810495364532	0.0
4	2.9988469433502816E-4	0.0	0.0	1.1005768282095534	0.0
5	2.9988469433502816E-4	0.0	0.0	1.9432528192909826	0.0
6	2.9988469433502816E-4	0.0	0.0	2.949066084090667	0.0
7	2.9988469433502816E-4	0.0	0.0	3.9394352871320977	0.0
8	2.9988469433502816E-4	0.0	0.0	5.323703036182588	0.0
9	2.9988469433502816E-4	0.0	0.0	6.103853068495163	0.0
10	4.4982704150254226E-4	0.0	0.0	7.409850912324211	0.0
11	5.997693886700563E-4	0.0	0.0	9.961869661115301	0.0
12	5.997693886700563E-4	0.0	0.0	12.123288595535017	0.0
13	5.997693886700563E-4	0.0	0.0	12.871500907900913	0.0
14	5.997693886700563E-4	0.0	0.0	13.178732777247149	0.0
15	5.997693886700563E-4	0.0	0.0	13.515653231332553	0.0
16	7.497117358375705E-4	0.0	0.0	14.226080072212234	0.0
17	7.497117358375705E-4	0.0	0.0	15.180762996627797	0.0
18	7.497117358375705E-4	0.0	0.0	16.111605087843724	0.0
19	7.497117358375705E-4	0.0	0.0	16.704477128544074	0.0
20	7.497117358375705E-4	0.0	0.0	17.296749399855756	0.0
21	7.497117358375705E-4	0.0	0.0	17.923958238057466	0.0
22	7.497117358375705E-4	0.0	0.0	18.510532700176782	0.0
23	7.497117358375705E-4	0.0	0.0	18.96005985698499	0.0
24	7.497117358375705E-4	0.0	0.0	19.228156773720503	0.0
25	7.497117358375705E-4	0.0	0.0	19.432078365868325	0.0
26	7.497117358375705E-4	0.0	0.0	19.62295497381257	0.0
27	7.497117358375705E-4	0.0	0.0	19.808883484300285	0.0
28	7.497117358375705E-4	0.0	0.0	19.99811072642569	0.0
29	7.497117358375705E-4	0.0	0.0	20.18388929456624	0.0
30	8.996540830050845E-4	0.0	0.0	20.411651719913692	0.0
31	8.996540830050845E-4	0.0	0.0	20.582735938031828	0.0
32	8.996540830050845E-4	0.0	0.0	20.809148882254775	0.0
33	8.996540830050845E-4	0.0	0.0	20.974235406486205	0.0
34	8.996540830050845E-4	0.0	0.0	21.140371527147813	0.0
35	8.996540830050845E-4	0.0	0.0	21.317603381499815	0.0
36	8.996540830050845E-4	0.0	0.0	21.468745267444667	0.0
37	8.996540830050845E-4	0.0	0.0	21.636080926883615	0.0
38	8.996540830050845E-4	0.0	0.0	21.84600021291813	0.0
39	8.996540830050845E-4	0.0	0.0	22.18981801497324	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACACGC	20	7.031155E-4	45.0	9
CGGGCTA	35	1.2109012E-7	45.0	6
TCGTCGA	20	7.031155E-4	45.0	19
CGTCGAG	20	7.031155E-4	45.0	20
GATCTAT	20	7.031155E-4	45.0	9
CGTTTTT	18480	0.0	44.366882	1
TTGTTCG	75	0.0	42.0	1
GTTTTTT	20750	0.0	40.53253	2
CTTTTCG	90	0.0	40.0	1
TTACGGG	90	0.0	40.0	3
GATAAGC	40	3.455316E-7	39.375	9
CGGGACG	40	3.455316E-7	39.375	6
GGACCGA	220	0.0	38.863636	8
CGGGTGG	70	0.0	38.571426	6
TTTACGG	70	0.0	38.571426	2
CTACACG	35	6.244347E-6	38.571426	28
TTTTACG	35	6.244347E-6	38.571426	1
AGGCACG	170	0.0	38.38235	10
TTCGTTG	100	0.0	38.25	1
GTTTGCG	120	0.0	37.500004	1
>>END_MODULE