##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933753.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	672418
Sequences flagged as poor quality	0
Sequence length	51
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.288787629123554	33.0	31.0	34.0	30.0	34.0
2	32.51857178124322	34.0	31.0	34.0	31.0	34.0
3	32.56077320952146	34.0	31.0	34.0	31.0	34.0
4	36.0571311297437	37.0	35.0	37.0	35.0	37.0
5	36.13291583509067	37.0	35.0	37.0	35.0	37.0
6	36.13984009946194	37.0	36.0	37.0	35.0	37.0
7	36.144625813110295	37.0	37.0	37.0	35.0	37.0
8	36.073665487836436	37.0	37.0	37.0	35.0	37.0
9	37.9045444946447	39.0	38.0	39.0	35.0	39.0
10	37.43594460588503	39.0	37.0	39.0	34.0	39.0
11	37.3910216561722	39.0	37.0	39.0	34.0	39.0
12	37.37585846898804	39.0	37.0	39.0	34.0	39.0
13	37.38311437231008	39.0	37.0	39.0	34.0	39.0
14	38.62588003295569	40.0	38.0	41.0	34.0	41.0
15	38.65327370772347	40.0	38.0	41.0	34.0	41.0
16	38.74750229767793	40.0	38.0	41.0	34.0	41.0
17	38.724121008063435	40.0	38.0	41.0	34.0	41.0
18	38.30534132042866	39.0	38.0	41.0	35.0	41.0
19	37.870153684166695	38.0	37.0	41.0	35.0	41.0
20	37.25222406300843	38.0	35.0	40.0	34.0	41.0
21	37.16010725471359	38.0	35.0	40.0	33.0	41.0
22	37.15623763789785	38.0	35.0	40.0	33.0	41.0
23	37.01613728365392	38.0	35.0	40.0	33.0	41.0
24	36.91754682355321	38.0	35.0	40.0	33.0	41.0
25	36.846519575621116	38.0	35.0	40.0	33.0	41.0
26	36.835557644203455	38.0	35.0	40.0	33.0	41.0
27	36.74977171937694	38.0	35.0	40.0	33.0	41.0
28	36.645689139791024	38.0	35.0	40.0	33.0	41.0
29	36.437602800638885	38.0	35.0	40.0	33.0	41.0
30	36.1195729442103	38.0	35.0	40.0	31.0	41.0
31	35.6810614825897	38.0	35.0	40.0	30.0	41.0
32	35.08736083805014	38.0	35.0	40.0	25.0	41.0
33	34.22135487152337	38.0	34.0	40.0	18.0	41.0
34	33.53857719454268	38.0	33.0	40.0	15.0	41.0
35	33.09029353765069	38.0	33.0	40.0	10.0	41.0
36	32.76355035112088	37.0	33.0	40.0	10.0	41.0
37	32.59932958368158	37.0	32.0	40.0	10.0	41.0
38	32.451000716816026	37.0	32.0	40.0	9.0	41.0
39	32.40629489394989	37.0	31.0	40.0	9.0	41.0
40	32.25315651871306	37.0	31.0	40.0	8.0	41.0
41	32.095688991074006	37.0	31.0	40.0	8.0	41.0
42	31.857506788931886	37.0	30.0	40.0	8.0	41.0
43	31.702316118842745	36.0	30.0	40.0	8.0	41.0
44	31.55248372292235	36.0	29.0	40.0	7.0	41.0
45	31.49628802322365	36.0	29.0	40.0	7.0	41.0
46	31.297126192338695	35.0	28.0	40.0	7.0	41.0
47	31.24755881014488	35.0	28.0	40.0	7.0	41.0
48	31.131901585025982	35.0	27.0	40.0	7.0	41.0
49	31.109491417540873	35.0	27.0	40.0	7.0	41.0
50	30.9399926831227	35.0	27.0	40.0	7.0	41.0
51	30.220093751208324	35.0	24.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	0.0
8	2.0
9	3.0
10	5.0
11	14.0
12	12.0
13	10.0
14	21.0
15	29.0
16	49.0
17	113.0
18	244.0
19	532.0
20	975.0
21	1582.0
22	2495.0
23	4345.0
24	8336.0
25	16673.0
26	26428.0
27	28656.0
28	25072.0
29	20750.0
30	18446.0
31	18058.0
32	19588.0
33	23055.0
34	33952.0
35	46397.0
36	61039.0
37	66171.0
38	113899.0
39	135422.0
40	44.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.116085530131556	3.875119345407172	37.595513504992425	39.41328161946884
2	43.59594775868582	3.8458220928053684	38.79670086166641	13.76152928684241
3	16.484389174590806	4.291973147655178	64.5936010041373	14.630036673616708
4	13.175732951824608	4.174486703211396	64.87423001763784	17.775550327326155
5	12.792489195708622	4.882825861294612	65.08198769217957	17.242697250817198
6	15.37272946292336	5.4008072359752415	66.47531743647552	12.751145864625874
7	61.1107971529614	1.718722580299754	33.95031066985119	3.2201695968876503
8	62.34172791329203	1.9435827119440585	32.434586819508105	3.280102555255808
9	57.53266569306592	4.633873572688417	33.985854037220896	3.847606697024767
10	23.86357890478839	25.69398796581888	39.18202665603835	11.260406473354372
11	19.539780315220593	18.7352212463081	46.37993034094864	15.34506809752267
12	16.227108732960748	18.238952556296827	49.576900082984096	15.95703862775833
13	17.23942547641497	18.03402050510248	50.90895841574735	13.817595602735203
14	15.85799309358167	19.260192320848045	50.82969224500237	14.052122340567921
15	13.55466391441038	18.674098551793676	51.86848061771101	15.902756916084934
16	15.282904383880263	19.904583161069453	49.325865756121935	15.486646698928345
17	15.020418846610292	19.64581554925656	48.97132438453462	16.362441219598523
18	16.32317992677174	17.80306297570856	49.86674955161819	16.00700754590151
19	15.977859010317989	19.90844980354482	48.89875047961239	15.21494070652481
20	16.889196898357866	22.064400417597387	47.35863703827084	13.687765645773908
21	15.545241204131955	23.341730887632394	47.878254300152584	13.234773608083067
22	15.283499251953398	20.279944915216426	48.094637561754745	16.341918271075432
23	13.45085943564866	22.844569895511423	46.94550116147991	16.759069507360007
24	13.371444547885392	22.17043565163336	47.78798307005464	16.67013673042661
25	14.173624144505354	23.753825745295337	45.34545476177021	16.727095348429103
26	14.579919038455246	22.798318902825326	45.92485626500183	16.696905793717598
27	14.579026736345547	22.094441255290608	47.41738026049273	15.909151747871118
28	12.81881210794476	22.311270667947618	47.44176985149118	17.428147372616436
29	15.141623216511157	21.11543712393184	46.67885154769801	17.064088111858993
30	17.376542567272143	20.776511039264268	46.95026010606498	14.89668628739861
31	17.618951307073875	23.13322962799925	43.829136043353984	15.41868302157289
32	16.793423138583442	23.419509888194547	43.23679615953172	16.550270813690293
33	17.20997950679488	23.41326377342665	41.29142884336826	18.085327876410208
34	17.177559196809128	23.746389894381174	41.52952478963978	17.546526119169922
35	18.096332935763172	25.41930763304968	38.74881398177919	17.735545449407958
36	18.96751722886657	26.18564642826337	38.58998420625266	16.256852136617404
37	18.17634269159957	26.20795398100586	38.85142872439465	16.764274602999922
38	17.649587012840225	28.132054763554816	37.24677209711816	16.971586126486798
39	19.303915124223327	26.111436636140017	36.72537023101702	17.85927800861964
40	21.810540467387845	24.808229404923722	36.40741324592739	16.973816881761046
41	20.337647118310336	25.15087341504838	34.434533281381526	20.076946185259764
42	21.329292196223182	24.980294995077468	35.6775101201931	18.01290268850626
43	19.980577557412204	25.087371248241418	37.20765952130966	17.724391673036713
44	19.08217804996297	25.824858941908158	36.56445841723452	18.528504590894354
45	20.422861969786652	24.564630928975724	35.69044850078374	19.322058600453886
46	19.970167366132376	24.878721271589992	36.54586878994911	18.605242572328525
47	17.611218022123143	27.28511134443159	37.670913033262046	17.43275760018322
48	17.7147250668483	26.61067966651696	37.93443958966	17.74015567697474
49	20.170340472741657	24.199828083126864	37.773527775877504	17.85630366825397
50	18.14570698583322	24.37650390084739	36.75377518150912	20.724013931810273
51	17.4000398561609	23.591872912384858	35.61846946393464	23.389617767519606
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	744.0
1	1710.5
2	2677.0
3	33114.5
4	63552.0
5	39922.5
6	16293.0
7	15559.0
8	14825.0
9	15339.0
10	15853.0
11	15542.0
12	15231.0
13	14683.0
14	14135.0
15	13386.5
16	12638.0
17	11749.5
18	10861.0
19	10150.0
20	9439.0
21	9041.0
22	8643.0
23	8448.5
24	8254.0
25	8162.0
26	8193.0
27	8316.0
28	9015.5
29	9715.0
30	10260.0
31	10805.0
32	12103.5
33	13402.0
34	14539.0
35	15676.0
36	16687.0
37	17698.0
38	18620.0
39	19542.0
40	22074.0
41	24606.0
42	27652.5
43	30699.0
44	33154.0
45	35609.0
46	40704.5
47	45800.0
48	49487.5
49	53175.0
50	51930.0
51	50685.0
52	44618.5
53	38552.0
54	33129.5
55	27707.0
56	23987.5
57	20268.0
58	17735.5
59	15203.0
60	13160.0
61	11117.0
62	9532.0
63	7947.0
64	6667.5
65	5388.0
66	4308.5
67	3229.0
68	2514.5
69	1800.0
70	1596.0
71	1392.0
72	1153.5
73	915.0
74	919.5
75	764.0
76	604.0
77	441.0
78	278.0
79	179.0
80	80.0
81	57.0
82	34.0
83	30.0
84	26.0
85	14.5
86	3.0
87	2.5
88	2.0
89	3.0
90	4.0
91	2.0
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	672418.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.92602290524307
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.70376274228497	33.25365216230696
2	9.217624270413852	8.097871496682467
3	4.248258377958888	5.5982728445283865
4	2.668706282570634	4.68902613182255
5	1.7798562247233702	3.909100264761912
6	1.259147210912209	3.3185597536561575
7	0.9344437997299918	2.8732479825421393
8	0.7153890375025231	2.51393561979965
9	0.5887019994089492	2.3273403759359907
>10	2.7482067060043147	19.48177922510365
>50	0.08992101505511015	2.762516880810905
>100	0.042576234662277716	3.324176569082632
>500	0.0023842691410874725	0.7425460578070383
>1k	6.812197545964207E-4	0.7744143451962986
>5k	0.0	0.0
>10k+	3.4060987729821033E-4	6.333560289963238
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	42332	6.295488817967396	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCC	3315	0.4929969156090408	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGC	1861	0.2767623710251659	No Hit
CCTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTTCTGC	984	0.1463375459907379	No Hit
CTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTTCTGCT	750	0.11153776371245266	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCG	729	0.10841470632850399	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	678	0.10083013839605721	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	677	0.10068142137777394	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.12016335077288234	0.0
2	0.0	0.0	0.0	0.43901263797221374	0.0
3	0.0	0.0	0.0	0.7044725156078511	0.0
4	0.0	0.0	0.0	1.097382877912251	0.0
5	0.0	0.0	0.0	1.9357007099750452	0.0
6	0.0	0.0	0.0	2.894925477902138	0.0
7	0.0	0.0	0.0	3.836899071708372	0.0
8	0.0	0.0	0.0	5.181895785062268	0.0
9	0.0	0.0	0.0	5.939460276197246	0.0
10	0.0	0.0	0.0	7.219913803616203	0.0
11	1.4871701828327022E-4	0.0	0.0	9.742451867737033	0.0
12	1.4871701828327022E-4	0.0	0.0	11.856166848597152	0.0
13	1.4871701828327022E-4	0.0	0.0	12.60733650794595	0.0
14	1.4871701828327022E-4	0.0	0.0	12.912355112444937	0.0
15	2.9743403656654044E-4	0.0	0.0	13.253809386423326	0.0
16	2.9743403656654044E-4	0.0	0.0	13.98430738023075	0.0
17	2.9743403656654044E-4	0.0	0.0	14.94115267586531	0.0
18	2.9743403656654044E-4	0.0	0.0	15.853680300051456	0.0
19	2.9743403656654044E-4	0.0	0.0	16.41999470567415	0.0
20	2.9743403656654044E-4	0.0	0.0	16.98853986657109	0.0
21	2.9743403656654044E-4	0.0	0.0	17.590248922545204	0.0
22	2.9743403656654044E-4	0.0	0.0	18.146896721979484	0.0
23	2.9743403656654044E-4	0.0	0.0	18.568063317757705	0.0
24	2.9743403656654044E-4	0.0	0.0	18.839620593142957	0.0
25	2.9743403656654044E-4	0.0	0.0	19.04261932309962	0.0
26	2.9743403656654044E-4	0.0	0.0	19.228069444898857	0.0
27	2.9743403656654044E-4	0.0	0.0	19.402514507345135	0.0
28	2.9743403656654044E-4	0.0	0.0	19.590344101436905	0.0
29	2.9743403656654044E-4	0.0	0.0	19.774158336035025	0.0
30	2.9743403656654044E-4	0.0	0.0	19.9966389953868	0.0
31	2.9743403656654044E-4	0.0	0.0	20.15442775178535	0.0
32	2.9743403656654044E-4	0.0	0.0	20.375867392009138	0.0
33	2.9743403656654044E-4	0.0	0.0	20.528004901712922	0.0
34	2.9743403656654044E-4	0.0	0.0	20.69337822604392	0.0
35	2.9743403656654044E-4	0.0	0.0	20.861428456704015	0.0
36	2.9743403656654044E-4	0.0	0.0	21.0108890600787	0.0
37	2.9743403656654044E-4	0.0	0.0	21.172544458952615	0.0
38	2.9743403656654044E-4	0.0	0.0	21.387143116335373	0.0
39	2.9743403656654044E-4	0.0	0.0	21.737669128429044	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAACCCG	25	3.8890714E-5	45.000004	23
TTCGTAA	20	7.031197E-4	45.0	20
TCTCGTC	20	7.031197E-4	45.0	42
GCGATCC	20	7.031197E-4	45.0	9
TCGTACG	20	7.031197E-4	45.0	30
GCTACGA	45	3.8380676E-10	45.0	10
CTACGGC	30	2.1641536E-6	44.999996	12
CGTCGAG	30	2.1641536E-6	44.999996	20
CGTTTTT	18110	0.0	44.3788	1
TACGAAT	50	1.0804797E-9	40.500004	12
TACGGGA	95	0.0	40.263157	4
CGAATAT	45	1.9263098E-8	40.0	14
GTTTTTT	20625	0.0	39.883633	2
GGCGCGA	40	3.4553705E-7	39.375	8
CGCTTTG	80	0.0	39.375	1
GTTGGGC	345	0.0	39.130432	4
GCGGGCA	110	0.0	38.86364	5
GCCATAC	35	6.2444233E-6	38.571426	17
ATATCGT	35	6.2444233E-6	38.571426	16
ACGCGCC	35	6.2444233E-6	38.571426	32
>>END_MODULE