FastQCFastQC Report
Sat 14 Jan 2017
SRR2933752.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933752.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1874881
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT699453.730636771080405No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCC40970.21852053543664907No Hit
CTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTGCT23710.1264613594142775TruSeq Adapter, Index 16 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTGC21540.11488729151343471TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGC21180.11296716964970044No Hit
CGCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTG19870.10598005953444511No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCT18870.10064638769073878No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT343600.044.115981
GTTTTTT391950.039.2709542
AGGCACG6650.038.90977510
TCGTTTG4700.037.819151
TGTTACG301.1401969E-437.4999961
TTGGGAC35650.036.921465
CGATAAC552.752131E-936.8181816
TTTGGGC46050.036.596094
TTGGGCG23000.036.5869565
TGGGCGA30800.036.5259746
ACGGGAC751.8189894E-1236.05
CTTGGGA45200.035.9900444
ACACGAC6200.035.92741826
TCTGGGA46600.035.536484
CACGACC6300.035.3571427
TACACGC17900.035.32122835
TTTGGGA93200.035.1984984
ACACGCG16400.035.12195236
ACGACCA6150.035.12195228
GTTTGCG3400.035.073531
Produced by FastQC (version 0.11.5)