##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933752.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1874881
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.390945345331254	33.0	31.0	34.0	31.0	34.0
2	32.57746384970566	34.0	31.0	34.0	31.0	34.0
3	32.660949148239276	34.0	31.0	34.0	31.0	34.0
4	36.15651500015201	37.0	35.0	37.0	35.0	37.0
5	36.15415751719709	37.0	35.0	37.0	35.0	37.0
6	36.11551613142381	37.0	35.0	37.0	35.0	37.0
7	36.17532099370573	37.0	36.0	37.0	35.0	37.0
8	36.04796677762482	37.0	36.0	37.0	35.0	37.0
9	37.87537182359841	39.0	38.0	39.0	35.0	39.0
10	37.40223726199156	39.0	37.0	39.0	34.0	39.0
11	37.320573945759755	39.0	37.0	39.0	34.0	39.0
12	37.30141699659872	39.0	37.0	39.0	34.0	39.0
13	37.30184209024466	39.0	37.0	39.0	34.0	39.0
14	38.51874598974548	40.0	38.0	41.0	34.0	41.0
15	38.56228048606818	40.0	38.0	41.0	34.0	41.0
16	38.59935377231942	40.0	38.0	41.0	34.0	41.0
17	38.52306679730607	40.0	38.0	41.0	34.0	41.0
18	38.19339414074813	39.0	38.0	41.0	34.0	41.0
19	37.85642715457674	39.0	37.0	41.0	34.0	41.0
20	37.46736032846885	39.0	35.0	41.0	34.0	41.0
21	37.34482508489872	39.0	35.0	41.0	33.0	41.0
22	37.367646799983575	39.0	35.0	41.0	33.0	41.0
23	37.29416053605536	39.0	35.0	40.0	33.0	41.0
24	37.243919480756375	39.0	35.0	41.0	33.0	41.0
25	37.16083367424386	39.0	35.0	40.0	33.0	41.0
26	37.107622830462304	38.0	35.0	40.0	33.0	41.0
27	37.01676639744069	38.0	35.0	40.0	33.0	41.0
28	36.84557046553888	38.0	35.0	40.0	33.0	41.0
29	36.67000785649863	38.0	35.0	40.0	32.0	41.0
30	36.44955226491708	38.0	35.0	40.0	31.0	41.0
31	36.09532658339383	38.0	35.0	40.0	30.0	41.0
32	35.50656975029349	38.0	35.0	40.0	27.0	41.0
33	34.92226173287798	38.0	34.0	40.0	23.0	41.0
34	34.32295276340205	38.0	34.0	40.0	19.0	41.0
35	33.951330777793366	38.0	33.0	40.0	16.0	41.0
36	33.781245316369414	38.0	33.0	40.0	15.0	41.0
37	33.70691579892271	38.0	33.0	40.0	15.0	41.0
38	33.67680348779469	38.0	33.0	40.0	15.0	41.0
39	33.7007324731543	38.0	33.0	40.0	15.0	41.0
40	33.60496266163026	38.0	33.0	40.0	13.0	41.0
41	33.4644065410018	38.0	33.0	40.0	12.0	41.0
42	33.31446155782687	38.0	33.0	40.0	10.0	41.0
43	33.16755570086848	38.0	33.0	40.0	10.0	41.0
44	32.937082406830086	37.0	33.0	40.0	10.0	41.0
45	32.9423440741039	37.0	33.0	40.0	10.0	41.0
46	32.81967015506584	37.0	32.0	40.0	10.0	41.0
47	32.75202266170493	37.0	32.0	40.0	10.0	41.0
48	32.694406204980474	37.0	31.0	40.0	10.0	41.0
49	32.70788012679205	37.0	32.0	40.0	10.0	41.0
50	32.628267607384146	36.0	31.0	40.0	10.0	41.0
51	31.652236061915396	35.0	29.0	39.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	7.0
10	7.0
11	11.0
12	9.0
13	20.0
14	35.0
15	61.0
16	136.0
17	231.0
18	462.0
19	890.0
20	1718.0
21	2902.0
22	4760.0
23	7866.0
24	14824.0
25	30155.0
26	51543.0
27	62360.0
28	58994.0
29	51293.0
30	47171.0
31	49345.0
32	55121.0
33	70726.0
34	105181.0
35	131080.0
36	171840.0
37	227746.0
38	362470.0
39	365814.0
40	102.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.41421242201505	3.574573532933557	42.85728000870455	32.15393403634684
2	36.21781862422201	4.048683623120614	43.57833910525522	16.155158647402153
3	18.441170399614695	4.557622590447074	60.23438287549984	16.766824134438398
4	15.226939736441938	4.344489063572568	60.15565787908672	20.272913320898766
5	15.130346939352417	5.081282492062162	59.36051408062698	20.427856487958433
6	17.93415155415197	6.033076232571561	60.52896157142773	15.503810641848737
7	67.66136090770561	1.9422566018856664	26.028585280879156	4.367797209529565
8	68.80484681427781	2.4905047307002417	24.442297937842454	4.26235051717949
9	62.7204606585698	4.877642901069454	26.978885593272317	5.423010847088428
10	28.822682612923167	24.971238174582812	32.107157734277536	14.098921478216484
11	22.504148263276445	18.92893468972164	41.309288429505656	17.257628617496255
12	19.23972774805441	18.455784660466453	43.76272414089214	18.541763450587
13	19.115026500348556	17.397584166675113	46.29792504164264	17.18946429133369
14	17.153195322796485	18.995018883865164	45.923181257903835	17.928604535434516
15	15.48988975833666	18.870050952567123	47.11984387275779	18.520215416338424
16	17.895055739537604	19.994708997531045	44.652113920830175	17.45812134210118
17	17.514444916770717	20.331637047897974	44.025834172942176	18.128083862389133
18	18.575418919920782	18.93928201309843	44.25390198097906	18.231397086001724
19	18.037464777764562	21.739939761510197	42.301618076027225	17.920977384698013
20	19.528066047925176	22.757337665697182	40.31759882360534	17.396997462772305
21	18.840022380087056	24.42224333171012	40.202871542247216	16.53486274595561
22	18.301694880901774	21.207052607605494	40.89246197492001	19.59879053657272
23	16.534382715489677	23.30718589606487	40.52310519974334	19.63532618870211
24	16.553370587253273	21.945286127492892	41.729955127818776	19.77138815743506
25	17.597703534250975	23.490397523896185	39.50602731586698	19.405871625985863
26	17.283656936093543	22.684053014564658	40.24682099823936	19.785469051102446
27	17.33480684907469	21.720365185843796	40.953852537841065	19.99097542724045
28	16.39432049287395	22.228397428956825	40.83843187914326	20.538850199025966
29	17.851426303856087	21.66308155024239	39.86349000283218	20.622002143069345
30	19.22591353797921	21.29820505941444	40.44550027441742	19.030381128188935
31	19.14345497127551	22.526976378767504	38.19522412355771	20.134344526399275
32	18.202541921327274	22.539937201347712	38.0845504328008	21.17297044452421
33	17.831585044597496	22.22413049148186	38.11137880217464	21.832905661746
34	16.82949477860195	22.831262357450953	38.28418976991073	22.055053094036367
35	16.295380880173195	24.195295594760413	37.25831132749225	22.251012197574138
36	16.60590725491378	24.806801071641345	35.599432710662704	22.98785896278217
37	16.726608248736856	23.549174587613827	35.0045149532157	24.71970221043362
38	16.615881221261507	23.94109279468937	35.59777927239116	23.845246711657968
39	17.502870848869875	22.561485235596283	34.49242911950145	25.443214796032386
40	19.138441319742427	22.73557628457486	34.27316187000669	23.85282052567603
41	17.668428022898517	23.582083342889497	32.88875400625426	25.860734627957722
42	18.815220806013823	24.241218509334725	33.3388092364262	23.604751448225247
43	18.611314531428928	23.34244146695177	34.96051216050512	23.085731841114182
44	19.70125037269032	24.100409572660876	34.01874572306189	22.17959433158691
45	20.0503391948609	22.850516912806732	34.23646620772198	22.862677684610382
46	19.797789833061405	23.629393012143172	33.93964736961973	22.6331697851757
47	18.36916582972466	24.39808179825813	34.75041882658152	22.482333545435683
48	18.519788722590928	23.543787579051685	35.19066010056105	22.74576359779634
49	19.889849009083775	22.125990929557663	34.862852629046856	23.121307432311706
50	18.329483311207483	22.216076646997863	35.497719588603225	23.95672045319143
51	18.006316134197316	21.458428561599376	34.93363045441284	25.601624849790465
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1060.0
1	2369.5
2	3679.0
3	59123.0
4	114567.0
5	73771.0
6	32975.0
7	31703.5
8	30432.0
9	30464.5
10	30497.0
11	29784.5
12	29072.0
13	27696.5
14	26321.0
15	24878.5
16	23436.0
17	22068.0
18	20700.0
19	19071.0
20	17442.0
21	16713.0
22	15984.0
23	15801.5
24	15619.0
25	16006.0
26	17671.5
27	18950.0
28	20927.0
29	22904.0
30	24939.5
31	26975.0
32	30493.5
33	34012.0
34	37496.0
35	40980.0
36	43895.5
37	46811.0
38	51028.0
39	55245.0
40	60072.0
41	64899.0
42	72477.0
43	80055.0
44	92037.5
45	104020.0
46	124195.5
47	144371.0
48	157675.5
49	170980.0
50	169460.5
51	167941.0
52	149253.5
53	130566.0
54	112947.5
55	95329.0
56	83855.0
57	72381.0
58	65390.5
59	58400.0
60	52257.0
61	46114.0
62	40639.5
63	35165.0
64	30931.0
65	26697.0
66	22392.5
67	18088.0
68	15022.0
69	11956.0
70	10051.0
71	8146.0
72	6820.5
73	5495.0
74	4994.0
75	3730.0
76	2967.0
77	2270.0
78	1573.0
79	1117.5
80	662.0
81	477.0
82	292.0
83	224.0
84	156.0
85	103.5
86	51.0
87	36.0
88	21.0
89	14.5
90	8.0
91	4.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1874881.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.25072637524322
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.22901235013741	27.27833901784275
2	8.566547116536825	6.38220205237484
3	4.048572548431353	4.524368046358123
4	2.571716054466494	3.831931642390058
5	1.868724727704549	3.480567675118652
6	1.524131567614557	3.4065004791048223
7	1.2383913040965073	3.229168293006573
8	1.0399133403119165	3.0990021835139503
9	0.9117113409575821	3.056571872569527
>10	4.816109228472432	27.405488108790166
>50	0.10413491681738812	2.678272478591603
>100	0.07400798048299277	5.194896454113356
>500	0.004302565695872627	1.0648050212817466
>1k	0.0025815394175235764	1.631105418932969
>5k	0.0	0.0
>10k+	1.434188565290876E-4	3.736781256010826
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	69945	3.730636771080405	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCC	4097	0.21852053543664907	No Hit
CTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTGCT	2371	0.1264613594142775	TruSeq Adapter, Index 16 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTGC	2154	0.11488729151343471	TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGC	2118	0.11296716964970044	No Hit
CGCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTG	1987	0.10598005953444511	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCT	1887	0.10064638769073878	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.13926217183917272	0.0
2	0.0	0.0	0.0	0.40658580464573485	0.0
3	0.0	0.0	0.0	0.684843464731895	0.0
4	0.0	0.0	0.0	1.0900958514167032	0.0
5	0.0	0.0	0.0	2.042849652857968	0.0
6	0.0	0.0	0.0	3.6747932268767993	0.0
7	0.0	0.0	0.0	5.191422815634699	0.0
8	0.0	0.0	0.0	7.273474956543908	0.0
9	0.0	0.0	0.0	8.839280999700781	0.0
10	1.0667343687412695E-4	0.0	0.0	10.781910958615507	0.0
11	1.0667343687412695E-4	0.0	0.0	12.89932534384849	0.0
12	1.0667343687412695E-4	0.0	0.0	14.572978231685104	0.0
13	1.0667343687412695E-4	0.0	0.0	15.35153431071092	0.0
14	1.0667343687412695E-4	0.0	0.0	15.788895401894841	0.0
15	1.6001015531119043E-4	0.0	0.0	16.16363918563365	0.0
16	1.6001015531119043E-4	0.0	0.0	16.705913601983273	0.0
17	1.6001015531119043E-4	0.0	0.0	17.33566023656968	0.0
18	1.6001015531119043E-4	0.0	0.0	17.99591547410209	0.0
19	1.6001015531119043E-4	0.0	0.0	18.423195925501407	0.0
20	1.6001015531119043E-4	0.0	0.0	18.866210708839656	0.0
21	1.6001015531119043E-4	0.0	0.0	19.31850608118595	1.6001015531119043E-4
22	1.6001015531119043E-4	0.0	0.0	19.789255958111475	1.6001015531119043E-4
23	1.6001015531119043E-4	0.0	0.0	20.20133544475623	1.6001015531119043E-4
24	1.6001015531119043E-4	0.0	0.0	20.528289528775428	1.6001015531119043E-4
25	1.6001015531119043E-4	0.0	0.0	20.818707960665236	1.6001015531119043E-4
26	1.6001015531119043E-4	0.0	0.0	21.093392060616114	1.6001015531119043E-4
27	2.6668359218531737E-4	0.0	0.0	21.378743504254402	1.6001015531119043E-4
28	2.6668359218531737E-4	0.0	0.0	21.66777518146485	1.6001015531119043E-4
29	2.6668359218531737E-4	0.0	0.0	21.961020459431825	1.6001015531119043E-4
30	2.6668359218531737E-4	0.0	0.0	22.330857265074425	1.6001015531119043E-4
31	2.6668359218531737E-4	0.0	0.0	22.586980187009203	1.6001015531119043E-4
32	2.6668359218531737E-4	0.0	0.0	22.917294484289936	1.6001015531119043E-4
33	2.6668359218531737E-4	0.0	0.0	23.207552906024436	1.6001015531119043E-4
34	2.6668359218531737E-4	0.0	0.0	23.49098422779899	1.6001015531119043E-4
35	2.6668359218531737E-4	0.0	0.0	23.8214051985166	1.6001015531119043E-4
36	2.6668359218531737E-4	0.0	0.0	24.100302899224005	1.6001015531119043E-4
37	3.2002031062238086E-4	0.0	0.0	24.380694028047646	1.6001015531119043E-4
38	3.2002031062238086E-4	0.0	0.0	24.724502515092958	1.6001015531119043E-4
39	3.2002031062238086E-4	0.0	0.0	25.178077968681745	1.6001015531119043E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	34360	0.0	44.11598	1
GTTTTTT	39195	0.0	39.270954	2
AGGCACG	665	0.0	38.909775	10
TCGTTTG	470	0.0	37.81915	1
TGTTACG	30	1.1401969E-4	37.499996	1
TTGGGAC	3565	0.0	36.92146	5
CGATAAC	55	2.752131E-9	36.81818	16
TTTGGGC	4605	0.0	36.59609	4
TTGGGCG	2300	0.0	36.586956	5
TGGGCGA	3080	0.0	36.525974	6
ACGGGAC	75	1.8189894E-12	36.0	5
CTTGGGA	4520	0.0	35.990044	4
ACACGAC	620	0.0	35.927418	26
TCTGGGA	4660	0.0	35.53648	4
CACGACC	630	0.0	35.35714	27
TACACGC	1790	0.0	35.321228	35
TTTGGGA	9320	0.0	35.198498	4
ACACGCG	1640	0.0	35.121952	36
ACGACCA	615	0.0	35.121952	28
GTTTGCG	340	0.0	35.07353	1
>>END_MODULE