FastQCFastQC Report
Sat 14 Jan 2017
SRR2933751.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933751.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1887226
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT693843.676507212172787No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCC40770.21603136031402703No Hit
CTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTGCT24340.12897236473003235TruSeq Adapter, Index 16 (95% over 22bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGC21130.11196327307911187No Hit
CCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTGC21100.1118043096057388TruSeq Adapter, Index 13 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTG20620.10926089403176939No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCT20020.10608162456430761No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACG253.8917613E-545.01
CGTTTTT354250.044.1298521
GTTTTTT408450.039.1002582
TACGGGA3150.036.4285744
TGGGCGA31100.036.318336
TTGGGAC34800.036.012935
CGTTCAT751.8189894E-1236.00000417
CGAACGA504.8818038E-836.022
CGTTAGC250.002107450536.026
GACACGA6950.035.93525325
TGGGAAC24200.035.7954566
CTTGGGA45950.035.6474464
TTGGGCG24400.035.502055
CGTTTTC3750.035.41
TCAAGCG7250.035.3793117
TCTGGGA44250.035.2372864
TTTGGGA92900.035.1910634
ACACGAC7150.034.9300726
CTTGTCG2000.034.8751
TCTTGCG4000.034.8751
Produced by FastQC (version 0.11.5)