FastQCFastQC Report
Sat 14 Jan 2017
SRR2933750.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933750.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1587714
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT766574.828136553560654No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCC20660.1301241911326599No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA16890.10637936051455112No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGA207.0341E-445.043
CGTTTTT460000.044.4864121
GTTTTTT519900.040.0014422
CGATAGT356.2497948E-638.57142610
AGTGCGC659.094947E-1238.0769213
TGGGCGA26000.036.6923076
CGTTTGG9250.036.2432442
TATAGCG250.002107350236.01
CCGCGCA250.002107350236.024
GCGTTTG3700.035.878381
GTTTGCG3150.035.7142871
CTTTGCG3000.035.251
TTGGGCG20200.035.086635
TTGGGAT53350.035.046865
CTTACGG900.035.02
ACGACCA4600.034.7282628
CCGCTTG653.6743586E-1034.6153831
TTGGGAC30300.034.603965
GCGATCT5150.034.514569
GGCGATC9000.034.5000048
Produced by FastQC (version 0.11.5)