##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933750.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1587714
Sequences flagged as poor quality	0
Sequence length	51
%GC	35
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.61264119356509	34.0	31.0	34.0	31.0	34.0
2	32.80917533006574	34.0	31.0	34.0	31.0	34.0
3	32.83813268636543	34.0	31.0	34.0	31.0	34.0
4	36.29646964125781	37.0	37.0	37.0	35.0	37.0
5	36.2976127942438	37.0	37.0	37.0	35.0	37.0
6	36.273216082997315	37.0	37.0	37.0	35.0	37.0
7	36.35854001413353	37.0	37.0	37.0	35.0	37.0
8	36.29175405646105	37.0	37.0	37.0	35.0	37.0
9	38.135293887942034	39.0	39.0	39.0	37.0	39.0
10	37.57008252115935	39.0	38.0	39.0	35.0	39.0
11	37.454814280153734	39.0	37.0	39.0	34.0	39.0
12	37.52557513506841	39.0	37.0	39.0	35.0	39.0
13	37.58247581113475	39.0	37.0	39.0	35.0	39.0
14	38.97733722824136	40.0	38.0	41.0	35.0	41.0
15	39.025370438252736	40.0	38.0	41.0	35.0	41.0
16	39.02456865657165	40.0	38.0	41.0	35.0	41.0
17	38.95061138215069	40.0	38.0	41.0	35.0	41.0
18	38.42887698918067	39.0	38.0	41.0	35.0	41.0
19	37.91192494366114	38.0	37.0	41.0	35.0	41.0
20	37.32825244345014	38.0	35.0	41.0	34.0	41.0
21	37.24820213212203	38.0	35.0	41.0	34.0	41.0
22	37.23813545764539	38.0	35.0	41.0	34.0	41.0
23	37.14287081930373	38.0	35.0	40.0	34.0	41.0
24	37.04205354364829	38.0	35.0	40.0	33.0	41.0
25	36.99514459153223	38.0	35.0	40.0	33.0	41.0
26	36.950870874729326	38.0	35.0	40.0	33.0	41.0
27	36.879479553622375	38.0	35.0	40.0	33.0	41.0
28	36.72751767635733	38.0	35.0	40.0	33.0	41.0
29	36.57690049971217	38.0	35.0	40.0	33.0	41.0
30	36.339489353876075	38.0	35.0	40.0	33.0	41.0
31	35.89319738945427	38.0	35.0	40.0	31.0	41.0
32	35.12606363614606	38.0	35.0	40.0	24.0	41.0
33	34.112156849407384	38.0	34.0	40.0	17.0	41.0
34	33.19835058455112	38.0	33.0	40.0	12.0	41.0
35	32.58985245453526	38.0	32.0	40.0	9.0	41.0
36	32.247477820312724	38.0	31.0	40.0	8.0	41.0
37	32.04419183807663	38.0	30.0	40.0	7.0	41.0
38	31.899649433084296	38.0	30.0	40.0	7.0	41.0
39	31.790101365863123	38.0	29.0	40.0	7.0	41.0
40	31.648255290310473	37.0	28.0	40.0	7.0	41.0
41	31.48672934798081	37.0	27.0	40.0	7.0	41.0
42	31.33045939004128	37.0	26.0	40.0	7.0	41.0
43	31.17689709859584	37.0	24.0	40.0	7.0	41.0
44	31.01544169793804	37.0	24.0	40.0	7.0	41.0
45	30.978845056477425	37.0	23.0	40.0	7.0	41.0
46	30.84685340054947	36.0	23.0	40.0	7.0	41.0
47	30.758632222176033	36.0	23.0	40.0	7.0	41.0
48	30.660807299047562	36.0	23.0	40.0	7.0	41.0
49	30.60952413343965	36.0	23.0	40.0	7.0	41.0
50	30.509514937828854	36.0	23.0	40.0	7.0	41.0
51	29.723467828588777	35.0	22.0	40.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	5.0
11	7.0
12	6.0
13	17.0
14	33.0
15	44.0
16	94.0
17	253.0
18	557.0
19	1123.0
20	2142.0
21	3392.0
22	5595.0
23	9775.0
24	20160.0
25	43563.0
26	74675.0
27	82098.0
28	68218.0
29	52918.0
30	43676.0
31	40478.0
32	40527.0
33	46680.0
34	67207.0
35	87180.0
36	109533.0
37	140385.0
38	272073.0
39	375173.0
40	125.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	17.89113152620686	3.161904473979571	40.35103299460734	38.59593100520623
2	43.46928980912179	3.1656205084794866	39.9196580744391	13.445431607959621
3	15.275862025528527	3.404769372821553	67.66602801260177	13.653340589048154
4	12.717970616874325	3.578793157961698	67.87582650275806	15.827409722405925
5	12.792921143228567	4.488213872271706	66.7601973655205	15.958667618979236
6	15.462923423236175	5.180404027425594	67.74091555532041	11.615756994017815
7	62.28590287671457	1.2958253186657043	33.88066112662608	2.537610677993644
8	64.92649179890081	1.4557407694332858	31.82178906276571	1.7959783689001925
9	60.48797201511103	3.965323729588578	32.93855190544393	2.6081523498564603
10	25.8065369455708	23.52533264807138	39.547613738998336	11.120516667359487
11	18.489853966142515	20.381567461142247	47.77151300549091	13.357065567224325
12	15.201100450081059	19.461628479688407	50.77482468505033	14.562446385180202
13	15.85102858575285	18.59182447216564	52.25242077603397	13.304726166047537
14	15.489880419269467	19.5123932899754	51.32454585649556	13.67318043425957
15	15.263643200223717	19.755321172452973	51.55443612640563	13.426599500917671
16	16.94007862877067	19.29856384714124	50.364864200983305	13.39649332310479
17	16.780667047087825	19.346746328368962	50.017824368872475	13.854762255670733
18	17.26488523751759	18.62640248810554	50.00314918177959	14.105563092597281
19	17.115110152080288	20.431198565988584	48.505776229220125	13.947915052711005
20	18.258137170800282	20.591365951298535	47.92626379813997	13.224233079761216
21	18.451622899338293	20.804187655963226	47.9621644704273	12.782024974271186
22	17.77536760398913	19.142868299958305	48.43460472100139	14.647159375051174
23	15.779479175720565	21.185364618564805	48.34296353121532	14.69219267449931
24	14.95760571488316	21.09806929963457	49.138635799646536	14.805689185835735
25	15.443272528931534	22.218800111355065	47.86422491708204	14.473702442631357
26	15.881008796294546	21.956662220021993	47.70500228630597	14.457326697377487
27	15.52080538434504	21.298357260816495	48.392027783341334	14.788809571497133
28	15.15600416699733	21.396359797797338	48.39221673424811	15.055419300957226
29	16.26413825159947	20.503251845105606	47.93401078531776	15.298599117977169
30	16.820787622959802	20.38975533376918	47.845014908226545	14.944442135044472
31	16.989898684523787	21.363671290925193	46.596616267161465	15.049813757389554
32	16.897501691110616	21.734833855467674	45.73348852501143	15.63417592841028
33	17.51499325445263	22.843093907340993	43.15178930210352	16.49012353610285
34	17.514174467189935	24.5352752447859	41.11074160711564	16.839808680908526
35	17.734113322676503	25.670933178141652	39.241387302751	17.353566196430844
36	18.16744073554809	27.096756720668836	37.42689174498682	17.308910798796255
37	18.078696792999242	27.13127175297314	37.358932402183264	17.431099051844352
38	18.102378639981758	27.707005165917792	36.77702659295062	17.41358960114983
39	18.396575201831062	27.059848310212043	36.24512979037786	18.298446697579035
40	18.962987036708125	27.101984362422954	36.19575062007389	17.73927798079503
41	18.277410163291375	27.306618194460714	36.117210026490916	18.298761615756995
42	18.726924370510055	27.62827562142804	35.68495333542439	17.959846672637518
43	18.470266055473466	27.11092803867699	36.17849310392174	18.240312801927804
44	18.652981582325282	27.387489182560586	35.70397439337311	18.25555484174102
45	18.878022112294783	26.984330931137475	35.52491191738562	18.61273503918212
46	18.61160133374147	27.273866703952983	35.477359272513816	18.63717268979174
47	18.080775252973773	27.302524258147248	36.004406335146	18.61229415373298
48	18.303611355697562	27.032072526916057	36.06613029802597	18.598185819360413
49	18.934455449785037	26.605673313959567	35.59167457111293	18.868196665142463
50	18.06628901678766	26.685095678440828	36.01303509322208	19.235580211549436
51	17.824809757928694	26.163717143011905	35.754802187295695	20.256670911763706
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	687.0
1	1891.5
2	3096.0
3	63822.5
4	124549.0
5	82585.0
6	40621.0
7	39640.5
8	38660.0
9	38549.0
10	38438.0
11	37931.5
12	37425.0
13	36288.0
14	35151.0
15	33799.0
16	32447.0
17	30668.0
18	28889.0
19	27182.0
20	25475.0
21	24449.5
22	23424.0
23	22831.5
24	22239.0
25	22448.5
26	23679.0
27	24700.0
28	26557.0
29	28414.0
30	31139.5
31	33865.0
32	37691.5
33	41518.0
34	44614.0
35	47710.0
36	53196.5
37	58683.0
38	61963.0
39	65243.0
40	68986.0
41	72729.0
42	76131.5
43	79534.0
44	82815.0
45	86096.0
46	90216.5
47	94337.0
48	96036.0
49	97735.0
50	93898.5
51	90062.0
52	82797.5
53	75533.0
54	68330.5
55	61128.0
56	54236.5
57	47345.0
58	41454.5
59	35564.0
60	30951.5
61	26339.0
62	22203.5
63	18068.0
64	14637.0
65	11206.0
66	8864.5
67	6523.0
68	5204.5
69	3886.0
70	3233.0
71	2580.0
72	2191.5
73	1803.0
74	1605.5
75	1221.5
76	1035.0
77	781.5
78	528.0
79	387.5
80	247.0
81	165.5
82	84.0
83	54.5
84	25.0
85	18.5
86	12.0
87	8.5
88	5.0
89	6.5
90	8.0
91	4.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1587714.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.64511671912669
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.22196519012164	32.078494853748225
2	8.61979159639972	7.351840374460269
3	4.078372335206729	5.2176799277684465
4	2.491596474349467	4.250176898623902
5	1.7848891281298136	3.8058402599898087
6	1.305241527443403	3.3397306370685076
7	1.066260847544992	3.182957280761379
8	0.8574904928545712	2.925422572265973
9	0.7217697474574492	2.7701959612191764
>10	3.7627770706138715	24.6546582381726
>50	0.05374524901827346	1.6166205640067242
>100	0.032993379683463345	2.751219141782742
>500	0.001775406386698059	0.5118159090720427
>1k	0.001183604257798706	0.7067753714774435
>5k	0.0	0.0
>10k+	1.4795053222483825E-4	4.836572009582788
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	76657	4.828136553560654	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCC	2066	0.1301241911326599	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	1689	0.10637936051455112	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.5193454236720214E-4	0.0	0.0	0.04547418489727999	0.0
2	2.5193454236720214E-4	0.0	0.0	0.13812311285281859	0.0
3	2.5193454236720214E-4	0.0	0.0	0.22926043355415396	0.0
4	2.5193454236720214E-4	0.0	0.0	0.3521415065937568	0.0
5	2.5193454236720214E-4	0.0	0.0	0.6082329689100178	0.0
6	2.5193454236720214E-4	0.0	0.0	1.0807991867552973	0.0
7	2.5193454236720214E-4	0.0	0.0	1.5403907756686657	0.0
8	2.5193454236720214E-4	0.0	0.0	2.174006149722179	0.0
9	2.5193454236720214E-4	0.0	0.0	2.599082706331241	0.0
10	3.149181779590027E-4	0.0	0.0	3.1015031674470337	0.0
11	3.149181779590027E-4	0.0	0.0	3.7373859523818522	0.0
12	3.149181779590027E-4	0.0	0.0	4.262921407759836	0.0
13	3.149181779590027E-4	0.0	0.0	4.5318615317368245	0.0
14	3.149181779590027E-4	0.0	0.0	4.678046549945393	0.0
15	3.149181779590027E-4	0.0	0.0	4.800171819357894	0.0
16	3.149181779590027E-4	0.0	0.0	4.9774077699132215	0.0
17	3.149181779590027E-4	0.0	0.0	5.192056000010077	0.0
18	3.149181779590027E-4	0.0	0.0	5.425788271691249	0.0
19	3.149181779590027E-4	0.0	0.0	5.582617524314832	0.0
20	3.149181779590027E-4	0.0	0.0	5.742218056904455	0.0
21	3.149181779590027E-4	0.0	0.0	5.9053456730872185	0.0
22	3.149181779590027E-4	0.0	0.0	6.077983818244344	0.0
23	3.149181779590027E-4	0.0	0.0	6.256353474240323	0.0
24	3.149181779590027E-4	0.0	0.0	6.407514199660644	0.0
25	3.149181779590027E-4	0.0	0.0	6.54903842883542	0.0
26	3.149181779590027E-4	0.0	0.0	6.693396921611827	0.0
27	3.149181779590027E-4	0.0	0.0	6.842542170693211	0.0
28	3.149181779590027E-4	0.0	0.0	6.994521683376226	0.0
29	3.779018135508032E-4	0.0	0.0	7.1594758249911505	0.0
30	3.779018135508032E-4	0.0	0.0	7.347041091783533	0.0
31	3.779018135508032E-4	0.0	0.0	7.512247167940826	0.0
32	3.779018135508032E-4	0.0	0.0	7.692758267546926	0.0
33	3.779018135508032E-4	0.0	0.0	7.866845036322663	0.0
34	3.779018135508032E-4	0.0	0.0	8.046600332301661	0.0
35	3.779018135508032E-4	0.0	0.0	8.233976648187268	0.0
36	3.779018135508032E-4	0.0	0.0	8.424124244038914	0.0
37	4.4088544914260377E-4	0.0	0.0	8.62019230163619	0.0
38	4.4088544914260377E-4	0.0	0.0	8.838367615326186	0.0
39	5.038690847344043E-4	0.0	0.0	9.093640290379753	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGCGA	20	7.0341E-4	45.0	43
CGTTTTT	46000	0.0	44.486412	1
GTTTTTT	51990	0.0	40.001442	2
CGATAGT	35	6.2497948E-6	38.571426	10
AGTGCGC	65	9.094947E-12	38.07692	13
TGGGCGA	2600	0.0	36.692307	6
CGTTTGG	925	0.0	36.243244	2
TATAGCG	25	0.0021073502	36.0	1
CCGCGCA	25	0.0021073502	36.0	24
GCGTTTG	370	0.0	35.87838	1
GTTTGCG	315	0.0	35.714287	1
CTTTGCG	300	0.0	35.25	1
TTGGGCG	2020	0.0	35.08663	5
TTGGGAT	5335	0.0	35.04686	5
CTTACGG	90	0.0	35.0	2
ACGACCA	460	0.0	34.72826	28
CCGCTTG	65	3.6743586E-10	34.615383	1
TTGGGAC	3030	0.0	34.60396	5
GCGATCT	515	0.0	34.51456	9
GGCGATC	900	0.0	34.500004	8
>>END_MODULE