FastQCFastQC Report
Sat 14 Jan 2017
SRR2933749.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933749.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1619841
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT764684.720710242548496No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCC20760.12816072688615734No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA18230.11254190997758422No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT463150.044.436471
CTGCGCG1350.040.0000041
GCCGATC900.040.09
CGATCCG451.9292202E-840.010
GTTTTTT527450.039.6890682
CGATACA301.1401284E-437.49999625
TGGGCGA26800.036.8563426
TTGGGCG21700.036.4976965
GTTAGCG250.002107362536.01
AGTGCGA250.002107362536.017
TCGAAGT250.002107362536.037
CTAGCGG751.8189894E-1236.02
GGCCGAT5400.035.8333368
GTTTGCG3050.035.4098361
ACACGCG7000.035.35714336
ACGGGCG702.1827873E-1135.3571435
TTGGGAT52500.035.1000025
TCTACGG900.035.02
GCGATAG900.035.09
CGCACGG458.7028457E-735.02
Produced by FastQC (version 0.11.5)