##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933749.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1619841
Sequences flagged as poor quality	0
Sequence length	51
%GC	35
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.52682269432617	34.0	31.0	34.0	31.0	34.0
2	32.74139251938925	34.0	31.0	34.0	31.0	34.0
3	32.7753081938289	34.0	31.0	34.0	31.0	34.0
4	36.21256592468026	37.0	37.0	37.0	35.0	37.0
5	36.26449694753991	37.0	37.0	37.0	35.0	37.0
6	36.25881861244406	37.0	37.0	37.0	35.0	37.0
7	36.278276695058345	37.0	37.0	37.0	35.0	37.0
8	36.236545438718984	37.0	37.0	37.0	35.0	37.0
9	38.09267637996569	39.0	39.0	39.0	37.0	39.0
10	37.569803456018214	39.0	38.0	39.0	35.0	39.0
11	37.45009541059894	39.0	37.0	39.0	34.0	39.0
12	37.49984041643594	39.0	37.0	39.0	35.0	39.0
13	37.56494557181847	39.0	37.0	39.0	35.0	39.0
14	38.976197046500246	40.0	38.0	41.0	35.0	41.0
15	39.01725539728899	40.0	38.0	41.0	35.0	41.0
16	39.06441187746205	40.0	38.0	41.0	35.0	41.0
17	39.01598675425551	40.0	38.0	41.0	35.0	41.0
18	38.52810985769591	39.0	38.0	41.0	35.0	41.0
19	38.04363391221731	39.0	37.0	41.0	35.0	41.0
20	37.41333007375415	39.0	35.0	41.0	34.0	41.0
21	37.36350975188306	38.0	35.0	41.0	34.0	41.0
22	37.3348983017469	38.0	35.0	41.0	34.0	41.0
23	37.18853517104456	38.0	35.0	41.0	34.0	41.0
24	37.097401535088935	38.0	35.0	41.0	34.0	41.0
25	37.05346574139067	38.0	35.0	41.0	33.0	41.0
26	37.03989774305009	38.0	35.0	41.0	34.0	41.0
27	36.96849937740803	38.0	35.0	40.0	33.0	41.0
28	36.87904553595075	38.0	35.0	40.0	33.0	41.0
29	36.68449495969049	38.0	35.0	40.0	33.0	41.0
30	36.36970850842768	38.0	35.0	40.0	33.0	41.0
31	35.92456481839884	38.0	35.0	40.0	31.0	41.0
32	35.23826721264618	38.0	35.0	41.0	24.0	41.0
33	34.17566045062448	38.0	34.0	41.0	18.0	41.0
34	33.295606791036896	38.0	33.0	41.0	12.0	41.0
35	32.7317378680994	38.0	33.0	41.0	10.0	41.0
36	32.37960268939976	38.0	31.0	41.0	8.0	41.0
37	32.19798486394652	38.0	31.0	41.0	7.0	41.0
38	32.05058335972481	38.0	30.0	41.0	7.0	41.0
39	31.97086010293603	38.0	30.0	41.0	7.0	41.0
40	31.8279923770296	38.0	29.0	41.0	7.0	41.0
41	31.67332040613863	38.0	28.0	40.0	7.0	41.0
42	31.47266799642681	37.0	27.0	40.0	7.0	41.0
43	31.35386559545042	37.0	25.0	40.0	7.0	41.0
44	31.20300140569352	37.0	24.0	40.0	7.0	41.0
45	31.138327774145733	37.0	24.0	40.0	7.0	41.0
46	30.981630913157527	37.0	23.0	40.0	7.0	41.0
47	30.948003538618913	37.0	23.0	40.0	7.0	41.0
48	30.81419596120854	36.0	23.0	40.0	7.0	41.0
49	30.753078234221753	36.0	24.0	40.0	7.0	41.0
50	30.604868008650232	36.0	23.0	40.0	7.0	41.0
51	29.916383151185826	35.0	23.0	40.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	7.0
10	11.0
11	7.0
12	9.0
13	16.0
14	31.0
15	48.0
16	101.0
17	259.0
18	532.0
19	1152.0
20	2110.0
21	3475.0
22	5615.0
23	9695.0
24	19896.0
25	43801.0
26	74722.0
27	82710.0
28	68997.0
29	53803.0
30	44539.0
31	40754.0
32	40839.0
33	46429.0
34	67429.0
35	87995.0
36	110242.0
37	138271.0
38	269537.0
39	406612.0
40	196.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.02355910240573	3.289582125653073	40.26142071968792	38.42543805225328
2	43.42154569491697	3.2245140109430492	39.88545789370685	13.46848240043313
3	15.421637061909163	3.493120621097997	67.33537427438866	13.749868042604183
4	12.949295640744987	3.668261267618241	67.48983387875724	15.892609212879535
5	12.921823808633071	4.509578409238931	66.57647262910372	15.992125153024277
6	15.512386709559767	5.249774514906093	67.53539390594509	11.702444869589053
7	62.322289656824346	1.3733446677791215	33.696208455027374	2.6081572203691596
8	64.99366295827801	1.507370167812767	31.652983224896765	1.845983649012465
9	60.57576021350244	4.043421545694917	32.72598977307032	2.654828467732327
10	25.847969029059026	23.62991182467909	39.3984965191028	11.123622627159085
11	18.58972578172796	20.449167541752555	47.608561581044064	13.35254509547542
12	15.27205447942113	19.555993458617234	50.617684081338844	14.55426798062279
13	15.92335297106321	18.662695906573546	52.06930803702339	13.344643085339857
14	15.573503819202008	19.576118890681247	51.134524931768	13.715852358348751
15	15.268720818895186	19.83361329908306	51.42486206979574	13.472803812226013
16	16.933452110423183	19.453020389038183	50.142575721938144	13.470951778600494
17	16.81183523568054	19.424005195571663	49.8677339319106	13.896425636837195
18	17.300216502730823	18.684241231083792	49.841681992244915	14.173860273940466
19	17.13038501927041	20.460773619139164	48.40925745181163	13.999583909778798
20	18.353529760019658	20.70005636355667	47.70548467411307	13.240929202310598
21	18.470269612881758	20.855688922554744	47.853338691883955	12.82070277267954
22	17.85712301392544	19.1760178931142	48.24461166250268	14.72224743045768
23	15.878965898504854	21.283385221142076	48.12472335247719	14.712925527875884
24	15.077035338653607	21.146581670670145	48.90912132734015	14.867261663336093
25	15.475222568140948	22.29490425294828	47.739438623914324	14.490434554996448
26	16.00712662539101	21.953883128035407	47.590967261601605	14.448022984971981
27	15.61480416905116	21.405866378243296	48.18942106046211	14.789908392243436
28	15.19105887553161	21.430621894371114	48.26905850635958	15.1092607237377
29	16.307032603817287	20.570846150949386	47.829015316935426	15.293105928297901
30	16.94530512562653	20.49028268823915	47.63127986018381	14.93313232595051
31	17.024448695890523	21.394075097494138	46.40424584882096	15.177230357794377
32	16.872767141960228	21.853626374440456	45.58064649555111	15.69295998804821
33	17.592467408838274	22.930028317594132	43.01619726874428	16.461307004823315
34	17.564810373363805	24.587783615799328	40.96358840157768	16.88381760925918
35	17.79575896646646	25.703880812993376	39.1475459628445	17.352814257695663
36	18.24395110384291	27.112722791928345	37.39428746401653	17.24903864021222
37	18.157893274710297	27.15920883592896	37.35959270076508	17.32330518859567
38	18.134928057753815	27.682902210772536	36.766695002781134	17.415474728692505
39	18.420635111717758	27.01894815602272	36.28214127189027	18.278275460369258
40	18.98359159942241	27.18643373022414	36.21732009499698	17.61265457535647
41	18.322045188385776	27.365648850720536	36.14681934831875	18.165486612574938
42	18.707515120311193	27.670246647664804	35.7219011001697	17.9003371318543
43	18.484036396164807	27.181741911706148	36.13712703901185	18.197094653117187
44	18.727949224646125	27.440903150370932	35.69010785626491	18.14103976871804
45	18.912164835931428	27.044259282238198	35.448849609313505	18.59472627251687
46	18.632137351752426	27.372069233955678	35.48206274566454	18.513730668627353
47	18.065785469067645	27.443002121813194	35.97859296066713	18.51261944845204
48	18.363592476051664	27.14729408627143	36.033104483711675	18.456008953965235
49	18.910930146847747	26.662123010838716	35.629793294527055	18.79715354778648
50	18.07541604392036	26.731018661708156	35.97649398922486	19.217071305146614
51	17.845516936538832	26.1680621740035	35.77097999124605	20.215440898211614
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	664.0
1	1851.5
2	3039.0
3	64675.0
4	126311.0
5	84231.5
6	42152.0
7	40550.0
8	38948.0
9	39184.0
10	39420.0
11	38712.5
12	38005.0
13	36961.5
14	35918.0
15	34314.0
16	32710.0
17	30868.5
18	29027.0
19	27339.0
20	25651.0
21	24642.0
22	23633.0
23	23138.0
24	22643.0
25	22514.0
26	23695.0
27	25005.0
28	26846.5
29	28688.0
30	31664.0
31	34640.0
32	38270.0
33	41900.0
34	45626.0
35	49352.0
36	54925.0
37	60498.0
38	63588.0
39	66678.0
40	70654.5
41	74631.0
42	78060.0
43	81489.0
44	84632.0
45	87775.0
46	92487.5
47	97200.0
48	98831.5
49	100463.0
50	95971.5
51	91480.0
52	84091.5
53	76703.0
54	69741.0
55	62779.0
56	55394.5
57	48010.0
58	42108.0
59	36206.0
60	31717.0
61	27228.0
62	22790.5
63	18353.0
64	14904.0
65	11455.0
66	9216.0
67	6977.0
68	5461.5
69	3946.0
70	3251.5
71	2557.0
72	2237.0
73	1917.0
74	1715.5
75	1257.0
76	1000.0
77	753.5
78	507.0
79	375.0
80	243.0
81	170.0
82	97.0
83	60.5
84	24.0
85	18.0
86	12.0
87	8.5
88	5.0
89	3.0
90	1.0
91	1.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1619841.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.4822116583649
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.21511627235074	31.953044893905304
2	8.57855250402342	7.288717663966385
3	3.9674375666982766	5.0563656734947315
4	2.480010543513873	4.214253312981318
5	1.821447218313175	3.868955312646014
6	1.3748301053354692	3.5043494117492116
7	1.0758048888346228	3.1991799693403324
8	0.8731838696021008	2.9675825572085244
9	0.7450231076144903	2.84852064132464
>10	3.7781898665780815	24.69624239715131
>50	0.0516212360777833	1.5067270578164496
>100	0.03540407276441836	2.848735116431296
>500	0.0020566293961665916	0.5674692236934145
>1k	0.0011752167978094808	0.7076983390171913
>5k	0.0	0.0
>10k+	1.469020997261851E-4	4.772158429273949
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	76468	4.720710242548496	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCC	2076	0.12816072688615734	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	1823	0.11254190997758422	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.556100876567515E-4	0.0	0.0	0.04537482382530137	0.0
2	5.556100876567515E-4	0.0	0.0	0.13698875383448128	0.0
3	5.556100876567515E-4	0.0	0.0	0.22687411912650687	0.0
4	5.556100876567515E-4	0.0	0.0	0.3510838409448829	0.0
5	5.556100876567515E-4	0.0	0.0	0.6103068140638495	0.0
6	5.556100876567515E-4	0.0	0.0	1.0604127195200024	0.0
7	5.556100876567515E-4	0.0	0.0	1.514160957773016	0.0
8	5.556100876567515E-4	0.0	0.0	2.1385432273908367	0.0
9	5.556100876567515E-4	0.0	0.0	2.555744668766873	0.0
10	5.556100876567515E-4	0.0	0.0	3.049867240056277	0.0
11	5.556100876567515E-4	0.0	0.0	3.676780622295645	0.0
12	5.556100876567515E-4	0.0	0.0	4.2013382794978025	0.0
13	5.556100876567515E-4	0.0	0.0	4.462968896329948	0.0
14	5.556100876567515E-4	0.0	0.0	4.60495814095334	0.0
15	6.17344541840835E-4	0.0	0.0	4.717932192110213	0.0
16	6.17344541840835E-4	0.0	0.0	4.894801403347612	0.0
17	6.17344541840835E-4	0.0	0.0	5.113156167796716	0.0
18	6.790789960249185E-4	0.0	0.0	5.350278206317781	0.0
19	6.790789960249185E-4	0.0	0.0	5.502638839244098	0.0
20	7.40813450209002E-4	0.0	0.0	5.663333623485268	0.0
21	7.40813450209002E-4	0.0	0.0	5.824028407726438	0.0
22	7.40813450209002E-4	0.0	0.0	5.990649699569278	0.0
23	8.025479043930855E-4	0.0	0.0	6.163444436830528	0.0
24	8.025479043930855E-4	0.0	0.0	6.302038286473796	0.0
25	8.025479043930855E-4	0.0	0.0	6.438903571399909	0.0
26	8.025479043930855E-4	0.0	0.0	6.5820657706527985	0.0
27	8.025479043930855E-4	0.0	0.0	6.721832574925564	0.0
28	8.025479043930855E-4	0.0	0.0	6.877773806194559	0.0
29	8.025479043930855E-4	0.0	0.0	7.034023709734474	0.0
30	8.025479043930855E-4	0.0	0.0	7.2134857680476046	0.0
31	8.025479043930855E-4	0.0	0.0	7.379983590982078	0.0
32	8.64282358577169E-4	0.0	0.0	7.562779309821149	0.0
33	9.87751266945336E-4	0.0	0.0	7.739648521058548	0.0
34	9.87751266945336E-4	0.0	0.0	7.91336927513256	0.0
35	9.87751266945336E-4	0.0	0.0	8.10252364275259	0.0
36	9.87751266945336E-4	0.0	0.0	8.289640773384548	0.0
37	0.0010494857211294196	0.0	0.0	8.480647174630102	0.0
38	0.0010494857211294196	0.0	0.0	8.690606053310171	0.0
39	0.0010494857211294196	0.0	0.0	8.940877530572445	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	46315	0.0	44.43647	1
CTGCGCG	135	0.0	40.000004	1
GCCGATC	90	0.0	40.0	9
CGATCCG	45	1.9292202E-8	40.0	10
GTTTTTT	52745	0.0	39.689068	2
CGATACA	30	1.1401284E-4	37.499996	25
TGGGCGA	2680	0.0	36.856342	6
TTGGGCG	2170	0.0	36.497696	5
GTTAGCG	25	0.0021073625	36.0	1
AGTGCGA	25	0.0021073625	36.0	17
TCGAAGT	25	0.0021073625	36.0	37
CTAGCGG	75	1.8189894E-12	36.0	2
GGCCGAT	540	0.0	35.833336	8
GTTTGCG	305	0.0	35.409836	1
ACACGCG	700	0.0	35.357143	36
ACGGGCG	70	2.1827873E-11	35.357143	5
TTGGGAT	5250	0.0	35.100002	5
TCTACGG	90	0.0	35.0	2
GCGATAG	90	0.0	35.0	9
CGCACGG	45	8.7028457E-7	35.0	2
>>END_MODULE