FastQCFastQC Report
Sat 14 Jan 2017
SRR2933748.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933748.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1526847
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT786025.147994527284004No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCC44420.29092633381078786No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGC23900.15653172845740274No Hit
CCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCTTCTGC20260.13269174972999914Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA16470.10786935429679594No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT494350.044.5994721
GTTTTTT549350.040.66672
TCGTGCG552.750312E-936.818181
CGGGTAT2400.036.5625046
TAATGCG250.002107324536.024
TGGGCGA20150.035.8436746
TTGGGTA15450.035.533985
GGCACCG5200.035.4807668
GCGGGAT6350.035.433075
TTGGGAC29150.035.4288145
GCACCGA4750.035.0526359
TGGCGAT1100.034.7727286
CGGGATC3300.034.7727286
TTGGGTC12150.034.6296275
TTGGGCG18000.034.6250045
TACGGGA1700.034.4117624
GCGATGC2950.034.3220339
TGCGGGA13250.034.3018884
TTGGGCA21850.034.290625
TAGGGCG1250.034.1999975
Produced by FastQC (version 0.11.5)