##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933748.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1526847
Sequences flagged as poor quality	0
Sequence length	51
%GC	35
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.550742805271256	34.0	31.0	34.0	31.0	34.0
2	32.75412729631718	34.0	31.0	34.0	31.0	34.0
3	32.763065323506545	34.0	31.0	34.0	31.0	34.0
4	36.25531110844767	37.0	37.0	37.0	35.0	37.0
5	36.26332369910017	37.0	37.0	37.0	35.0	37.0
6	36.25004208018223	37.0	37.0	37.0	35.0	37.0
7	36.31132981890131	37.0	37.0	37.0	35.0	37.0
8	36.22750544095119	37.0	37.0	37.0	35.0	37.0
9	38.05883955628822	39.0	39.0	39.0	37.0	39.0
10	37.51342668911816	39.0	37.0	39.0	34.0	39.0
11	37.495255254783224	39.0	37.0	39.0	35.0	39.0
12	37.54699390312192	39.0	37.0	39.0	35.0	39.0
13	37.57148686148645	39.0	37.0	39.0	35.0	39.0
14	38.9404066026262	40.0	38.0	41.0	35.0	41.0
15	38.98593834221766	40.0	38.0	41.0	35.0	41.0
16	39.00420998305658	40.0	38.0	41.0	35.0	41.0
17	38.96471290181662	40.0	38.0	41.0	35.0	41.0
18	38.3841969758594	39.0	38.0	40.0	35.0	41.0
19	37.795699241639795	38.0	37.0	40.0	35.0	41.0
20	37.12218578547818	38.0	35.0	40.0	34.0	41.0
21	37.02984319974431	38.0	35.0	40.0	34.0	41.0
22	37.02347975926861	38.0	35.0	40.0	34.0	41.0
23	36.95259446427835	38.0	35.0	40.0	33.0	41.0
24	36.843725664719514	37.0	35.0	40.0	33.0	41.0
25	36.783271670311436	37.0	35.0	40.0	33.0	41.0
26	36.71741896863274	37.0	35.0	40.0	33.0	41.0
27	36.636464557352504	37.0	35.0	40.0	33.0	41.0
28	36.4808576104875	37.0	35.0	40.0	33.0	41.0
29	36.31016794741058	37.0	35.0	40.0	33.0	41.0
30	36.02583690441806	37.0	35.0	40.0	31.0	41.0
31	35.50151783381046	37.0	35.0	40.0	30.0	41.0
32	34.59651163476104	37.0	35.0	40.0	21.0	41.0
33	33.424601155191056	37.0	33.0	40.0	15.0	41.0
34	32.426195944976804	37.0	31.0	40.0	10.0	41.0
35	31.760137066778793	37.0	30.0	40.0	7.0	41.0
36	31.403589226687416	37.0	27.0	40.0	7.0	41.0
37	31.16844320354299	37.0	25.0	40.0	7.0	41.0
38	31.020624201377085	37.0	24.0	40.0	7.0	41.0
39	30.900668501821073	37.0	23.0	40.0	7.0	41.0
40	30.741699070044348	37.0	23.0	40.0	7.0	41.0
41	30.56583338081681	36.0	22.0	40.0	7.0	41.0
42	30.408271424707255	36.0	22.0	40.0	7.0	41.0
43	30.24806152810334	36.0	21.0	40.0	7.0	41.0
44	30.102180506625746	36.0	21.0	40.0	7.0	41.0
45	30.064494346846804	36.0	20.0	40.0	7.0	41.0
46	29.90385939128151	35.0	20.0	40.0	7.0	41.0
47	29.79852729186356	35.0	20.0	40.0	7.0	41.0
48	29.727643306762236	35.0	20.0	40.0	7.0	41.0
49	29.72912020654329	35.0	20.0	40.0	7.0	41.0
50	29.63086871179627	35.0	20.0	40.0	7.0	41.0
51	28.793951194847946	35.0	20.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	12.0
11	2.0
12	11.0
13	14.0
14	20.0
15	54.0
16	90.0
17	220.0
18	578.0
19	1277.0
20	2327.0
21	3803.0
22	6254.0
23	11092.0
24	22779.0
25	49602.0
26	83438.0
27	90052.0
28	72054.0
29	54423.0
30	43209.0
31	39188.0
32	38658.0
33	44055.0
34	62934.0
35	86643.0
36	109873.0
37	135486.0
38	253649.0
39	314958.0
40	88.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	16.260437358818532	2.8776950146281846	37.68773164567242	43.17413598088086
2	46.895006506873315	3.3674624896928114	37.534802111802954	12.202728891630924
3	13.909121215157775	3.3634018339755065	69.97459470398802	12.7528822468787
4	11.93623198657102	3.476117777354247	69.27773378734085	15.309916448733892
5	11.12848897106259	4.293422982132459	68.98641448684774	15.59167355995722
6	14.202339854615426	4.946599102595087	69.95750065330711	10.893560389482378
7	57.573024671103255	1.42849938467967	38.07185657764007	2.926619366577005
8	59.65024655384593	1.5506465284340867	36.309990457459065	2.4891164602609166
9	54.89240244765847	4.17042441056635	37.67201297837963	3.2651601633955467
10	23.25832254312318	20.179756059382505	44.70539615298717	11.856525244507145
11	18.124081849720373	18.485414714113464	48.790939760172435	14.599563675993732
12	14.512259578071674	16.404263164547594	53.48440282490648	15.599074432474241
13	15.757767477684403	16.459605972307635	54.660814082877984	13.121812467129976
14	14.515992761553711	17.809773998311552	54.130571039534416	13.543662200600323
15	13.462449086254221	17.752204379351696	54.84884864036803	13.936497894026054
16	14.137958813162028	17.525593592547256	53.58297196772172	14.753475626569001
17	14.485341360332765	17.497103508079068	52.2417766809641	15.775778450624065
18	14.816284801293122	17.105512209147346	53.16898156789777	14.909221421661764
19	14.99095849158429	18.609264713491267	52.01660677199483	14.383170022929606
20	15.760714727801803	19.085998793592285	51.23460307417835	13.918683404427556
21	15.752200449684873	19.28222015696399	51.50240986817932	13.463169525171809
22	15.116838818820746	18.288800384059435	50.99024329222247	15.604117504897348
23	13.748463336536012	19.414649928905778	50.611816377148465	16.225070357409745
24	13.891765186688646	19.227859765909745	51.185875205570696	15.694499841830911
25	13.977693901222585	20.200714282439563	50.09748848443885	15.724103331899006
26	13.97815236235196	20.70967163049081	50.32671904912542	14.985456958031811
27	14.234170155883334	19.884769069854414	50.933394112180196	14.947666662082055
28	13.944226238778345	19.372995460579876	50.93103631208628	15.751741988555501
29	14.951268856669989	18.804569154604227	50.300914236986415	15.94324775173937
30	16.080196640527834	19.40377785069493	49.215212788183756	15.300812720593484
31	15.603266077085653	21.388521574198332	47.4572108403789	15.551001508337114
32	15.601694210356376	21.53608056340943	46.436610871947224	16.42561435428697
33	16.472835850612405	22.049098567177982	43.94408870043953	17.53397688177008
34	16.57553114359199	22.551048009394524	42.00086845636793	18.87255239064556
35	17.874024050870847	24.133459344649463	38.80460845127246	19.187908153207232
36	18.5059799704882	25.709321235199074	37.527925194862355	18.25677359945037
37	17.57320805555501	26.514706450613584	37.878647958832815	18.033437534998594
38	17.659857209006535	26.9814853747625	37.24243490015699	18.116222516073975
39	18.109476588027484	25.774750187805328	36.930026387712715	19.18574683645447
40	19.1774290416787	25.055686653607072	36.11802623314582	19.6488580715684
41	19.277439062329098	25.337378270383343	35.02243512283811	20.362747544449444
42	19.449034513608765	25.692227184518163	35.278190938581275	19.580547363291803
43	18.32665617445625	25.7712789821115	36.84494910099047	19.057115742441777
44	18.393526004897677	25.739776153078864	36.282875756379	19.583822085644467
45	19.310644746985126	24.973294639214014	35.716610767156105	19.99944984664475
46	19.44602176904431	24.95299136062749	36.29171750673119	19.309269363597007
47	18.222192531406225	25.41426875122393	36.95006768851103	19.413471028858815
48	18.17614993512775	24.825735650002915	37.58660821942212	19.41150619544722
49	18.83554802806044	23.80710051498284	37.52772871152119	19.82962274543553
50	18.131286238896234	23.521872198065687	37.13338664581323	21.213454917224844
51	17.62488317428007	23.46371312908235	36.32734648592819	22.58405721070939
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	830.0
1	1871.5
2	2913.0
3	65608.5
4	128304.0
5	85107.5
6	41911.0
7	41524.0
8	41137.0
9	41448.5
10	41760.0
11	41038.0
12	40316.0
13	39306.5
14	38297.0
15	36301.5
16	34306.0
17	32734.5
18	31163.0
19	29112.0
20	27061.0
21	25487.0
22	23913.0
23	22665.5
24	21418.0
25	21198.5
26	20983.5
27	20988.0
28	21950.5
29	22913.0
30	23733.5
31	24554.0
32	26862.5
33	29171.0
34	31907.5
35	34644.0
36	38000.5
37	41357.0
38	44397.0
39	47437.0
40	52743.5
41	58050.0
42	64074.5
43	70099.0
44	75958.5
45	81818.0
46	90529.5
47	99241.0
48	105241.0
49	111241.0
50	107286.0
51	103331.0
52	90841.5
53	78352.0
54	68132.0
55	57912.0
56	50870.5
57	43829.0
58	38956.5
59	34084.0
60	29600.5
61	25117.0
62	21531.5
63	17946.0
64	14795.0
65	11644.0
66	9535.5
67	7427.0
68	5893.0
69	4359.0
70	3659.5
71	2960.0
72	2311.0
73	1662.0
74	1378.0
75	910.5
76	727.0
77	495.5
78	264.0
79	229.0
80	194.0
81	128.5
82	63.0
83	49.0
84	35.0
85	26.0
86	17.0
87	11.0
88	5.0
89	3.5
90	2.0
91	1.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1526847.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.30999731344363
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.1427185143422	32.63908213093557
2	8.089668917870613	6.8454774036350825
3	3.806766708580251	4.831928676388112
4	2.2167176231918972	3.7515726672764926
5	1.5787499690575677	3.339845347471245
6	1.193853585830888	3.030716520548995
7	0.9048574083131651	2.679916016034571
8	0.7606375158595351	2.5746057002017095
9	0.6288261114080103	2.3945067975866507
>10	3.556745286826442	25.023258116946966
>50	0.0721551792712206	2.1233192505843173
>100	0.043349034373976365	3.370669839976406
>500	0.002477072537066284	0.6946601924873906
>1k	0.0023222555034996414	1.5517680301769243
>5k	0.0	0.0
>10k+	1.5481703356664276E-4	5.14867330974954
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	78602	5.147994527284004	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCC	4442	0.29092633381078786	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGC	2390	0.15653172845740274	No Hit
CCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCTTCTGC	2026	0.13269174972999914	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	1647	0.10786935429679594	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.9648334115991977E-4	0.0	0.0	0.10904825434375547	0.0
2	2.6197778821322634E-4	0.0	0.0	0.39447305460206555	0.0
3	2.6197778821322634E-4	0.0	0.0	0.6388328365579524	0.0
4	2.6197778821322634E-4	0.0	0.0	0.913451053052467	0.0
5	3.274722352665329E-4	0.0	0.0	1.5568030064570975	0.0
6	3.274722352665329E-4	0.0	0.0	2.400895440080113	0.0
7	3.274722352665329E-4	0.0	0.0	3.164036737145241	0.0
8	3.274722352665329E-4	0.0	0.0	4.159224860120235	0.0
9	3.274722352665329E-4	0.0	0.0	4.772973323456771	0.0
10	3.274722352665329E-4	0.0	0.0	5.689764593308956	0.0
11	3.9296668231983954E-4	0.0	0.0	7.401789439282391	0.0
12	3.9296668231983954E-4	0.0	0.0	8.729754847735235	0.0
13	3.9296668231983954E-4	0.0	0.0	9.264713491266644	0.0
14	3.9296668231983954E-4	0.0	0.0	9.500820972893813	0.0
15	3.9296668231983954E-4	0.0	0.0	9.759131072072055	0.0
16	4.584611293731461E-4	0.0	0.0	10.295858065673903	0.0
17	4.584611293731461E-4	0.0	0.0	10.97084383700528	0.0
18	4.584611293731461E-4	0.0	0.0	11.65539179760644	0.0
19	4.584611293731461E-4	0.0	0.0	12.053991002372864	0.0
20	5.239555764264527E-4	0.0	0.0	12.414865405636583	0.0
21	5.239555764264527E-4	0.0	0.0	12.816673838308619	0.0
22	5.239555764264527E-4	0.0	0.0	13.163466935455878	0.0
23	5.239555764264527E-4	0.0	0.0	13.455768652654784	0.0
24	5.239555764264527E-4	0.0	0.0	13.664957916543045	0.0
25	5.239555764264527E-4	0.0	0.0	13.823585467306154	0.0
26	5.894500234797593E-4	0.0	0.0	13.973698739952333	0.0
27	6.549444705330658E-4	0.0	0.0	14.111302573211331	0.0
28	7.859333646396791E-4	0.0	0.0	14.25152618435246	0.0
29	7.859333646396791E-4	0.0	0.0	14.397185834599014	0.0
30	7.859333646396791E-4	0.0	0.0	14.561642391149867	0.0
31	7.859333646396791E-4	0.0	0.0	14.708349952549273	0.0
32	8.514278116929856E-4	0.0	0.0	14.858856191877772	0.0
33	8.514278116929856E-4	0.0	0.0	15.002354525371567	0.0
34	9.169222587462922E-4	0.0	0.0	15.142905608747963	0.0
35	9.169222587462922E-4	0.0	0.0	15.293280859182355	0.0
36	9.169222587462922E-4	0.0	0.0	15.439267981664175	0.0
37	9.169222587462922E-4	0.0	0.0	15.593965865604085	0.0
38	9.169222587462922E-4	0.0	0.0	15.74794331062641	0.0
39	9.169222587462922E-4	0.0	0.0	15.912465361624315	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	49435	0.0	44.599472	1
GTTTTTT	54935	0.0	40.6667	2
TCGTGCG	55	2.750312E-9	36.81818	1
CGGGTAT	240	0.0	36.562504	6
TAATGCG	25	0.0021073245	36.0	24
TGGGCGA	2015	0.0	35.843674	6
TTGGGTA	1545	0.0	35.53398	5
GGCACCG	520	0.0	35.480766	8
GCGGGAT	635	0.0	35.43307	5
TTGGGAC	2915	0.0	35.428814	5
GCACCGA	475	0.0	35.052635	9
TGGCGAT	110	0.0	34.772728	6
CGGGATC	330	0.0	34.772728	6
TTGGGTC	1215	0.0	34.629627	5
TTGGGCG	1800	0.0	34.625004	5
TACGGGA	170	0.0	34.411762	4
GCGATGC	295	0.0	34.322033	9
TGCGGGA	1325	0.0	34.301888	4
TTGGGCA	2185	0.0	34.29062	5
TAGGGCG	125	0.0	34.199997	5
>>END_MODULE