FastQCFastQC Report
Sat 14 Jan 2017
SRR2933747.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933747.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1534145
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT779745.082570421961418No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCC43570.28400183815741015No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGC24410.15911142688598534No Hit
CCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCTTCTGC20610.13434192986973198Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA16080.1048140821108826No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT492500.044.529441
GTTTTTT549000.040.3278662
GGTCGTT403.4592267E-739.3758
CGGGCTC1150.039.1304326
TACGCGG356.2496565E-638.571432
GCGACGG301.14010036E-437.4999962
GGGTACG850.037.0588267
TACGGGT850.037.0588264
TAGGGCG1350.036.6666645
GCGGGAC5100.036.176475
TCCGCGA250.002107328336.023
CCGAATA250.002107328336.023
GGGACCG7400.035.878387
TTGGGAC26600.035.8646625
TGGGCGA21100.035.722756
CTTTGCG3100.035.5645181
GACCGAT5950.035.546229
GGACCGA6150.035.4878048
TTGGGCG16800.035.3571435
TAGCGGG2550.035.2941173
Produced by FastQC (version 0.11.5)