##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2933747.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1534145 Sequences flagged as poor quality 0 Sequence length 51 %GC 35 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.47266523047039 34.0 31.0 34.0 31.0 34.0 2 32.70045790978037 34.0 31.0 34.0 31.0 34.0 3 32.711342800061274 34.0 31.0 34.0 31.0 34.0 4 36.182365421782166 37.0 37.0 37.0 35.0 37.0 5 36.238547855645976 37.0 37.0 37.0 35.0 37.0 6 36.24385765361162 37.0 37.0 37.0 35.0 37.0 7 36.23829690153147 37.0 37.0 37.0 35.0 37.0 8 36.185565901528214 37.0 37.0 37.0 35.0 37.0 9 38.02696876761975 39.0 39.0 39.0 35.0 39.0 10 37.5205114249305 39.0 37.0 39.0 34.0 39.0 11 37.50399734053821 39.0 37.0 39.0 35.0 39.0 12 37.525641318128336 39.0 37.0 39.0 35.0 39.0 13 37.56346890287424 39.0 37.0 39.0 35.0 39.0 14 38.95383226487718 40.0 38.0 41.0 35.0 41.0 15 38.99180781477631 40.0 38.0 41.0 35.0 41.0 16 39.06007450403971 40.0 38.0 41.0 35.0 41.0 17 39.040440766681115 40.0 38.0 41.0 35.0 41.0 18 38.50298570213376 39.0 38.0 41.0 35.0 41.0 19 37.94264362234339 38.0 37.0 41.0 35.0 41.0 20 37.23127800827171 38.0 35.0 40.0 34.0 41.0 21 37.16940901935606 38.0 35.0 40.0 34.0 41.0 22 37.142103256211115 38.0 35.0 40.0 34.0 41.0 23 37.01649648501282 38.0 35.0 40.0 34.0 41.0 24 36.91120526416994 38.0 35.0 40.0 33.0 41.0 25 36.86056142020474 38.0 35.0 40.0 33.0 41.0 26 36.83872384944057 38.0 35.0 40.0 33.0 41.0 27 36.75328472862735 38.0 35.0 40.0 33.0 41.0 28 36.66059727079253 37.0 35.0 40.0 33.0 41.0 29 36.444287208836194 37.0 35.0 40.0 33.0 41.0 30 36.073483275700795 37.0 35.0 40.0 32.0 41.0 31 35.55249080106509 37.0 35.0 40.0 30.0 41.0 32 34.731523421840826 37.0 35.0 40.0 21.0 41.0 33 33.495468811618196 37.0 33.0 40.0 15.0 41.0 34 32.5130747093658 37.0 32.0 40.0 10.0 41.0 35 31.898692105374653 37.0 30.0 40.0 7.0 41.0 36 31.532552007795875 37.0 28.0 40.0 7.0 41.0 37 31.33855013704702 37.0 25.0 40.0 7.0 41.0 38 31.181755309960923 37.0 24.0 40.0 7.0 41.0 39 31.08774007672026 37.0 24.0 40.0 7.0 41.0 40 30.926826994840773 37.0 23.0 40.0 7.0 41.0 41 30.761908424562215 37.0 23.0 40.0 7.0 41.0 42 30.542828089913275 37.0 22.0 40.0 7.0 41.0 43 30.409475636266453 36.0 22.0 40.0 7.0 41.0 44 30.27131855202735 36.0 21.0 40.0 7.0 41.0 45 30.194110074341083 36.0 20.0 40.0 7.0 41.0 46 30.02770989704363 35.0 20.0 40.0 7.0 41.0 47 29.991487114972834 35.0 20.0 40.0 7.0 41.0 48 29.891666693826203 35.0 20.0 40.0 7.0 41.0 49 29.88899028449071 35.0 20.0 40.0 7.0 41.0 50 29.739570901055636 35.0 20.0 40.0 7.0 41.0 51 29.011269469313525 35.0 20.0 39.0 7.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 2.0 8 1.0 9 11.0 10 3.0 11 14.0 12 7.0 13 17.0 14 31.0 15 41.0 16 104.0 17 250.0 18 557.0 19 1265.0 20 2285.0 21 3783.0 22 6091.0 23 10617.0 24 22163.0 25 49285.0 26 82818.0 27 89151.0 28 72192.0 29 54064.0 30 43671.0 31 38971.0 32 38215.0 33 43183.0 34 61668.0 35 85054.0 36 107438.0 37 129858.0 38 249036.0 39 342150.0 40 149.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 16.34741175051902 3.030808691486137 37.6362729728937 42.98550658510115 2 46.97671993194907 3.4218408299085157 37.40317896939337 12.198260268749042 3 13.995222094391337 3.4445244745444534 69.77899742201681 12.781256009047384 4 12.179422414439314 3.5466660582930554 68.94908890619857 15.324822621069064 5 11.22019105104146 4.310609492583817 68.86982651574655 15.599372940628168 6 14.242134869911254 4.953377940155591 69.85584804565408 10.948639144279062 7 57.56483252886787 1.4962731684423571 37.94595686848375 2.9929374342060235 8 59.689142812446015 1.586681832551682 36.2030968389559 2.5210785160463973 9 54.936006700800775 4.214399551541738 37.50532055314198 3.3442731945155115 10 23.402090415182396 20.062184474088173 44.641608192185224 11.894116918544205 11 18.174162155467705 18.57666648198182 48.610463808831625 14.638707553718847 12 14.578087468915909 16.545437360875276 53.367119796368655 15.509355373840153 13 15.766110765279684 16.521254509840986 54.58656124421094 13.126073480668385 14 14.647442060561419 17.8763415452907 53.95122364574405 13.524992748403832 15 13.506415625641644 17.824716698877875 54.730289509792094 13.938578165688382 16 14.1583096773773 17.61476262022169 53.48184167728605 14.745086025114967 17 14.564268696896319 17.584257029159563 52.07108845643664 15.780385817507472 18 14.909477265838628 17.154897353248877 53.1131020861783 14.8225232947342 19 14.988544107629984 18.68343605069925 51.93576878326365 14.392251058407126 20 15.856454246502125 19.04031235639395 51.20324350045139 13.899989896652531 21 15.801439889971286 19.26584514501563 51.4506125561795 13.482102408833585 22 15.209318545509062 18.342008089196263 50.848061949815694 15.60061141547898 23 13.84086901824795 19.503045670389696 50.44620945217043 16.20987585919193 24 14.078851738264635 19.257110638173053 51.03748341910315 15.626554204459161 25 14.079894664454795 20.21067109041192 50.042271102144845 15.667163142988441 26 14.07233344957615 20.737935462423696 50.149496951070475 15.040234136929689 27 14.303537149356806 19.940683572934763 50.808104840155266 14.947674437553166 28 13.991441486952017 19.49346378601762 50.837046041932155 15.678048685098215 29 15.066698389004952 18.912032435004512 50.08444442995936 15.936824746031178 30 16.12253079076619 19.496462198814324 49.08766772371582 15.29333928670367 31 15.71996128136519 21.415641937365766 47.35875683198133 15.505639949287714 32 15.694539955480089 21.614645291025294 46.37117091278856 16.31964384070606 33 16.5414612047753 22.1327188759863 43.803942912827665 17.521877006410737 34 16.641516936143585 22.603469685068884 41.917224251944894 18.837789126842637 35 17.967010940947564 24.176658660035393 38.7672612432332 19.089069155783843 36 18.569235632876943 25.758060678749402 37.44489601699969 18.22780767137396 37 17.669190330770558 26.603547904533144 37.86832405020386 17.858937714492438 38 17.72264029801616 27.027692949493037 37.241590592805764 18.008076159685036 39 18.15682350755633 25.886536148799493 36.86046625318989 19.096174090454294 40 19.190298179116056 25.11359747611862 36.19736074491003 19.498743599855295 41 19.297132930720366 25.40991887989727 35.03906084496576 20.253887344416597 42 19.474691114594776 25.71966795837421 35.33427413966737 19.471366787363646 43 18.285820440701496 25.837583800748952 36.89507836612576 18.981517392423793 44 18.420292736345 25.81470460745236 36.23471053909507 19.530292117107575 45 19.169309289539125 25.224343200936026 35.737625843710994 19.868721665813858 46 19.432713335440912 25.035052097422344 36.297090561843895 19.235144005292852 47 18.283147942339216 25.53682996066213 36.964432957771265 19.215589139227387 48 18.21646584905599 24.941775386290082 37.495412754335476 19.346346010318452 49 18.85877801641957 23.951908066056337 37.50212659168462 19.687187325839474 50 18.193456289985626 23.677748843818545 37.02596560299059 21.10282926320524 51 17.671015451603335 23.68909066613651 36.22623676380003 22.41365711846012 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 722.0 1 1875.5 2 3029.0 3 65761.0 4 128493.0 5 85709.5 6 42926.0 7 42172.5 8 41419.0 9 41664.0 10 41909.0 11 41229.5 12 40550.0 13 39135.5 14 37721.0 15 36237.5 16 34754.0 17 32785.0 18 30816.0 19 28738.5 20 26661.0 21 25269.5 22 23878.0 23 22568.5 24 21259.0 25 21182.5 26 20992.0 27 20878.0 28 22032.0 29 23186.0 30 23749.5 31 24313.0 32 27041.5 33 29770.0 34 32425.0 35 35080.0 36 38565.0 37 42050.0 38 44943.5 39 47837.0 40 53357.5 41 58878.0 42 64866.0 43 70854.0 44 76527.0 45 82200.0 46 91129.0 47 100058.0 48 105482.0 49 110906.0 50 107353.5 51 103801.0 52 91065.0 53 78329.0 54 68305.5 55 58282.0 56 50987.5 57 43693.0 58 39042.0 59 34391.0 60 29867.5 61 25344.0 62 21786.5 63 18229.0 64 15049.0 65 11869.0 66 9727.5 67 7586.0 68 6049.5 69 4513.0 70 3687.0 71 2861.0 72 2259.5 73 1658.0 74 1343.5 75 875.5 76 722.0 77 501.0 78 280.0 79 228.5 80 177.0 81 121.0 82 65.0 83 49.0 84 33.0 85 24.5 86 16.0 87 10.0 88 4.0 89 5.0 90 6.0 91 4.5 92 3.0 93 1.5 94 0.0 95 0.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1534145.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.155939017773726 #Duplication Level Percentage of deduplicated Percentage of total 1 76.99129045007244 32.456401451129594 2 8.169788729484004 6.888102309364457 3 3.740039382217607 4.729946163625127 4 2.3044562877384234 3.885860749401049 5 1.6466469452181227 3.470797410320929 6 1.1994785745367065 3.0339087384777335 7 0.8656331245112148 2.554410404606179 8 0.7198780877372286 2.4277709413505737 9 0.6111182883030395 2.318603876491428 >10 3.629893149940292 25.310474450624042 >50 0.0706378657651284 2.051887150169661 >100 0.04646178703405355 3.5565648166474744 >500 0.0021827528061292762 0.6457551020557442 >1k 0.002338663720852796 1.5446199138332868 >5k 0.0 0.0 >10k+ 1.559109147235197E-4 5.124896521902758 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 77974 5.082570421961418 No Hit CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCC 4357 0.28400183815741015 No Hit CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGC 2441 0.15911142688598534 No Hit CCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCTTCTGC 2061 0.13434192986973198 Illumina Single End Adapter 1 (95% over 21bp) CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 1608 0.1048140821108826 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.9554866065463173E-4 0.0 0.0 0.09875207363058902 0.0 2 1.9554866065463173E-4 0.0 0.0 0.3792340358962158 0.0 3 1.9554866065463173E-4 0.0 0.0 0.630839979271842 0.0 4 1.9554866065463173E-4 0.0 0.0 0.8995890218981909 0.0 5 1.9554866065463173E-4 0.0 0.0 1.5456166138142091 0.0 6 1.9554866065463173E-4 0.0 0.0 2.366529891242353 0.0 7 1.9554866065463173E-4 0.0 0.0 3.104595719439818 0.0 8 1.9554866065463173E-4 0.0 0.0 4.075234088042525 0.0 9 1.9554866065463173E-4 0.0 0.0 4.6813045702981135 0.0 10 1.9554866065463173E-4 0.0 0.0 5.592170231627389 0.0 11 1.9554866065463173E-4 0.0 0.0 7.267631156116273 0.0 12 1.9554866065463173E-4 0.0 0.0 8.574678403931832 0.0 13 1.9554866065463173E-4 0.0 0.0 9.122084287991031 0.0 14 3.2591443442438623E-4 0.0 0.0 9.355439023038892 0.0 15 3.2591443442438623E-4 0.0 0.0 9.599874848857182 0.0 16 3.2591443442438623E-4 0.0 0.0 10.114298192152631 0.0 17 3.9109732130926346E-4 0.0 0.0 10.783139794478357 0.0 18 4.5628020819414074E-4 0.0 0.0 11.447874874930335 0.0 19 4.5628020819414074E-4 0.0 0.0 11.833431650854385 0.0 20 4.5628020819414074E-4 0.0 0.0 12.18607106890157 0.0 21 4.5628020819414074E-4 0.0 0.0 12.56295852086993 0.0 22 4.5628020819414074E-4 0.0 0.0 12.903995385051608 0.0 23 4.5628020819414074E-4 0.0 0.0 13.188257954756558 0.0 24 4.5628020819414074E-4 0.0 0.0 13.386870211094779 0.0 25 4.5628020819414074E-4 0.0 0.0 13.550283708515167 0.0 26 4.5628020819414074E-4 0.0 0.0 13.693294962340588 0.0 27 4.5628020819414074E-4 0.0 0.0 13.831287133875872 0.0 28 5.214630950790179E-4 0.0 0.0 13.973255461511135 0.0 29 5.214630950790179E-4 0.0 0.0 14.112681656557887 0.0 30 5.214630950790179E-4 0.0 0.0 14.27042424281929 0.0 31 5.866459819638952E-4 0.0 0.0 14.410241535187351 0.0 32 5.866459819638952E-4 0.0 0.0 14.555860104488168 0.0 33 5.866459819638952E-4 0.0 0.0 14.699066906974243 0.0 34 5.866459819638952E-4 0.0 0.0 14.83334365395709 0.0 35 5.866459819638952E-4 0.0 0.0 14.98196063605461 0.0 36 5.866459819638952E-4 0.0 0.0 15.1285569486587 0.0 37 5.866459819638952E-4 0.0 0.0 15.274436249507055 0.0 38 5.866459819638952E-4 0.0 0.0 15.4276812165734 0.0 39 5.866459819638952E-4 0.0 0.0 15.589986604916746 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTTTTT 49250 0.0 44.52944 1 GTTTTTT 54900 0.0 40.327866 2 GGTCGTT 40 3.4592267E-7 39.375 8 CGGGCTC 115 0.0 39.130432 6 TACGCGG 35 6.2496565E-6 38.57143 2 GCGACGG 30 1.14010036E-4 37.499996 2 GGGTACG 85 0.0 37.058826 7 TACGGGT 85 0.0 37.058826 4 TAGGGCG 135 0.0 36.666664 5 GCGGGAC 510 0.0 36.17647 5 TCCGCGA 25 0.0021073283 36.0 23 CCGAATA 25 0.0021073283 36.0 23 GGGACCG 740 0.0 35.87838 7 TTGGGAC 2660 0.0 35.864662 5 TGGGCGA 2110 0.0 35.72275 6 CTTTGCG 310 0.0 35.564518 1 GACCGAT 595 0.0 35.54622 9 GGACCGA 615 0.0 35.487804 8 TTGGGCG 1680 0.0 35.357143 5 TAGCGGG 255 0.0 35.294117 3 >>END_MODULE