FastQCFastQC Report
Sat 14 Jan 2017
SRR2933746.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933746.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1785915
Sequences flagged as poor quality0
Sequence length51
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT934545.2328358292527914No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCC27450.15370272381384334No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA21420.11993851891047445No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA18380.1029164321930215No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAATC207.034338E-445.06
GCGACCG207.034338E-445.031
CGTTTTT596200.044.4414631
GTTTTTT670500.040.036912
TATTGCG403.4596633E-739.3751
GACCGAT7200.037.81259
GGACCGA7800.037.788468
CGTTTGG7450.037.4496652
CGACCAA5700.037.10526329
GGGACCG8900.036.910117
TGGGCGA29050.036.7125636
GCGAGAC5950.036.6806721
TTGGGCG22000.036.6136325
GCGTTTG3400.036.3970571
TCAAGCG6200.036.2903217
CACGACC5900.036.22881327
GGGCGAT43700.036.0926787
CTGCGCG1500.036.0000041
CGAGACA6000.036.00000422
TACGCGG504.881622E-836.02
Produced by FastQC (version 0.11.5)