##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933746.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1785915
Sequences flagged as poor quality	0
Sequence length	51
%GC	34
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.63714846451259	34.0	31.0	34.0	31.0	34.0
2	32.84223717254181	34.0	31.0	34.0	31.0	34.0
3	32.84161844208711	34.0	31.0	34.0	31.0	34.0
4	36.3091020569288	37.0	37.0	37.0	35.0	37.0
5	36.315434945112166	37.0	37.0	37.0	35.0	37.0
6	36.29862003510805	37.0	37.0	37.0	35.0	37.0
7	36.37495289529457	37.0	37.0	37.0	35.0	37.0
8	36.31012618181716	37.0	37.0	37.0	35.0	37.0
9	38.1491633140435	39.0	39.0	39.0	37.0	39.0
10	37.63142814747622	39.0	38.0	39.0	35.0	39.0
11	37.51501779200018	39.0	37.0	39.0	35.0	39.0
12	37.57798159486874	39.0	37.0	39.0	35.0	39.0
13	37.61755626667563	39.0	37.0	39.0	35.0	39.0
14	39.042761833569905	40.0	39.0	41.0	35.0	41.0
15	39.07801435118693	41.0	39.0	41.0	35.0	41.0
16	39.08066341343233	41.0	39.0	41.0	35.0	41.0
17	39.01768617207426	41.0	38.0	41.0	35.0	41.0
18	38.420672316431634	39.0	38.0	40.0	35.0	41.0
19	37.810412589624924	38.0	37.0	40.0	35.0	41.0
20	37.14456679069273	38.0	35.0	40.0	34.0	41.0
21	37.05666003141247	38.0	35.0	40.0	34.0	41.0
22	37.047461385340284	38.0	35.0	40.0	34.0	41.0
23	36.94932121629529	38.0	35.0	40.0	33.0	41.0
24	36.84082053177223	37.0	35.0	40.0	33.0	41.0
25	36.78175613061092	37.0	35.0	40.0	33.0	41.0
26	36.736915250725815	37.0	35.0	40.0	33.0	41.0
27	36.66370907909951	37.0	35.0	40.0	33.0	41.0
28	36.5044013852843	37.0	35.0	40.0	33.0	41.0
29	36.33245759176668	37.0	35.0	40.0	33.0	41.0
30	36.04934725336872	37.0	35.0	40.0	32.0	41.0
31	35.532233616941454	37.0	35.0	40.0	30.0	41.0
32	34.62572630836294	37.0	35.0	40.0	21.0	41.0
33	33.43902369373682	37.0	33.0	40.0	15.0	41.0
34	32.358302606787	37.0	31.0	40.0	10.0	41.0
35	31.646203766696622	37.0	29.0	40.0	7.0	41.0
36	31.262205648085157	37.0	25.0	40.0	7.0	41.0
37	31.020730549886192	37.0	23.0	40.0	7.0	41.0
38	30.851201764921623	37.0	23.0	40.0	7.0	41.0
39	30.71119678148176	37.0	23.0	40.0	7.0	41.0
40	30.56138674013041	36.0	22.0	40.0	7.0	41.0
41	30.40276552915452	36.0	21.0	40.0	7.0	41.0
42	30.235442896218466	36.0	21.0	40.0	7.0	41.0
43	30.071697141241323	36.0	20.0	40.0	7.0	41.0
44	29.900994168255487	36.0	19.0	40.0	7.0	41.0
45	29.861184322882107	35.0	20.0	40.0	7.0	41.0
46	29.719982194001393	35.0	20.0	40.0	7.0	41.0
47	29.625364589020194	35.0	20.0	40.0	7.0	41.0
48	29.512629660426168	35.0	20.0	40.0	7.0	41.0
49	29.458142184818428	35.0	20.0	40.0	7.0	41.0
50	29.349881153358364	35.0	18.0	40.0	7.0	41.0
51	28.623164596299375	35.0	18.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	6.0
11	2.0
12	8.0
13	21.0
14	35.0
15	50.0
16	140.0
17	336.0
18	741.0
19	1509.0
20	2772.0
21	4479.0
22	7399.0
23	13031.0
24	26798.0
25	58581.0
26	99561.0
27	107691.0
28	87646.0
29	65729.0
30	52853.0
31	47592.0
32	46626.0
33	51960.0
34	71918.0
35	93767.0
36	116251.0
37	147098.0
38	283571.0
39	397625.0
40	114.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	16.400052634083927	2.940509486733691	38.04201207784245	42.617425801339934
2	46.53810511698485	2.914080457356593	37.14667271398695	13.401141711671608
3	13.998314589440147	3.1097224671946875	69.21342841064664	13.678534532718523
4	11.767357349033968	3.3825797980307013	69.26561454492514	15.58444830801018
5	11.709963800068872	4.098739301702489	69.03570438682692	15.15559251140172
6	14.503545801451917	4.712262341712792	69.93221961851488	10.851972238320414
7	58.50496804159212	1.1912101079838626	38.02924551280436	2.2745763376196515
8	61.080286575788875	1.3104207087123407	36.03083013469286	1.5784625808059176
9	57.00181699576967	3.6906011764277693	37.038996816757795	2.2685850110447587
10	23.538858232334686	23.185985895185382	42.93541405945972	10.339741813020217
11	17.153223977624915	19.085006845230595	51.16850466007621	12.593264517068281
12	14.37783993079178	18.274161984192975	53.58485706206623	13.763141022949021
13	14.844099523213591	17.66198279313405	55.07686536033349	12.417052323318858
14	14.820190210620327	18.52495779474387	54.088240481769844	12.566611512865954
15	14.10767029785852	18.640920760506518	54.40421296646257	12.84719597517239
16	15.753717282177485	18.615331636724033	53.23573630323951	12.39521477785897
17	15.941016229775773	18.03467690231618	53.10258326964049	12.921723598267555
18	16.43224901521069	17.06402600347721	53.18108644588349	13.322638535428618
19	15.901596660535356	19.091782083693793	51.789586850438006	13.21703440533284
20	17.119851728665697	19.368838942502865	51.11850228034369	12.39280704848775
21	17.25518851681071	19.51083898169846	51.380720806981294	11.853251694509536
22	16.980595381079166	17.857064865909074	51.57692275388246	13.585416999129299
23	14.84152381272345	20.049554430082058	51.41902050209557	13.689901255098928
24	14.15873655801088	20.139984265768526	52.13294025751506	13.568338918705539
25	14.569394400069433	21.328730650674864	50.82576718376855	13.276107765487158
26	14.922378724631352	21.150950633148835	50.61293510609407	13.31373553612574
27	14.52919091894071	20.220447221732275	51.30546526570413	13.944896593622877
28	13.99389108664186	20.395427553942937	51.416836747549574	14.193844611865627
29	14.913251750503242	19.499248284492822	50.97039892716059	14.617101037843346
30	15.64648933459879	19.43250378657439	50.53891142635568	14.382095452471141
31	15.707466480767563	20.400690962335833	49.125854253981856	14.765988302914753
32	15.529462488416302	20.969922980656975	47.94287522082518	15.557739310101546
33	16.11969214660272	22.553089032792712	45.021571575354926	16.30564724524963
34	15.957422385723843	24.38828275701811	42.77907963144942	16.875215225808617
35	16.315782106091277	25.612305176898115	40.4412304057024	17.63068231130821
36	17.18099685595339	26.866676185596738	38.36492778211729	17.58739917633258
37	17.316277650392095	27.33679934375376	37.758739917633264	17.588183088220884
38	17.36510416229216	27.737714280914826	37.94603886523154	16.95114269156147
39	17.920449741449058	27.46032145986791	37.042244451723626	17.5769843469594
40	18.65043969057878	27.116128147196257	36.997953430034464	17.2354787321905
41	17.96350890159946	26.97328820240605	37.0352452384352	18.02795765755929
42	18.403395458350484	27.530481573871096	36.65650380897188	17.40961915880655
43	18.20249004011949	27.217252780787437	36.733103199200414	17.84715397989266
44	18.263747154819796	27.498397180156953	36.27373083265441	17.964124832368842
45	18.681964147229852	26.671930075059564	36.036261524204676	18.609844253505905
46	18.480162829697942	27.01998695346643	35.888886089203574	18.610964127632055
47	17.79933535470613	27.384561975233986	36.39277345226397	18.42332921779592
48	18.088990797434366	27.064389962568207	36.39792487324425	18.448694366753177
49	18.742269368922933	26.585979735877686	36.10278204729788	18.568968847901495
50	18.06082596316174	26.610728954065564	36.151384584372714	19.17706049839998
51	17.76389133861354	26.19295991130597	35.94907932348404	20.09406942659645
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	554.0
1	2013.0
2	3472.0
3	77872.0
4	152272.0
5	102177.0
6	52082.0
7	50955.5
8	49829.0
9	49505.0
10	49181.0
11	48616.0
12	48051.0
13	47109.0
14	46167.0
15	44108.5
16	42050.0
17	39982.0
18	37914.0
19	35853.0
20	33792.0
21	31980.5
22	30169.0
23	28994.5
24	27820.0
25	27928.0
26	28353.0
27	28670.0
28	30154.0
29	31638.0
30	34711.5
31	37785.0
32	41013.0
33	44241.0
34	48576.0
35	52911.0
36	57814.5
37	62718.0
38	66671.5
39	70625.0
40	73799.5
41	76974.0
42	79721.0
43	82468.0
44	85839.5
45	89211.0
46	93265.5
47	97320.0
48	100060.5
49	102801.0
50	99308.0
51	95815.0
52	88551.5
53	81288.0
54	73377.5
55	65467.0
56	58342.5
57	51218.0
58	44736.0
59	38254.0
60	32723.0
61	27192.0
62	22719.0
63	18246.0
64	14693.5
65	11141.0
66	8881.5
67	6622.0
68	5209.5
69	3797.0
70	3012.0
71	2227.0
72	2049.0
73	1871.0
74	1819.5
75	1499.5
76	1231.0
77	906.0
78	581.0
79	404.5
80	228.0
81	194.5
82	161.0
83	94.5
84	28.0
85	22.5
86	17.0
87	11.0
88	5.0
89	3.0
90	1.0
91	2.0
92	3.0
93	3.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1785915.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.43357199758462
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.55498228111416	35.08287709526345
2	9.197658349045486	8.541602623191777
3	4.335605403879075	6.039529370224081
4	2.686669322682402	4.990066137139008
5	1.85427377065611	4.305027731649661
6	1.341078458051597	3.7362637881809158
7	1.0327986344929556	3.356957082761501
8	0.8079173927826196	3.0011592340698097
9	0.6229303525577987	2.6032393237475993
>10	2.482771528329718	16.751568773757548
>50	0.04566518353942959	1.4782168274448342
>100	0.0343809339363783	3.300290078007629
>500	0.001815771629136412	0.5746195818685187
>1k	0.001331565861366702	0.9856724041520437
>5k	0.0	0.0
>10k+	1.2105144194242745E-4	5.252909948541578
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	93454	5.2328358292527914	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCC	2745	0.15370272381384334	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	2142	0.11993851891047445	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	1838	0.1029164321930215	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.919559441518773E-4	0.0	0.0	0.03897161958995809	0.0
2	3.919559441518773E-4	0.0	0.0	0.12884151821335282	0.0
3	3.919559441518773E-4	0.0	0.0	0.2123841280240101	0.0
4	3.919559441518773E-4	0.0	0.0	0.32425955322621736	0.0
5	3.919559441518773E-4	0.0	0.0	0.5661523644742331	0.0
6	3.919559441518773E-4	0.0	0.0	1.009678512135236	0.0
7	3.919559441518773E-4	0.0	0.0	1.4083537010440026	0.0
8	3.919559441518773E-4	0.0	0.0	1.9667229403415056	0.0
9	3.919559441518773E-4	0.0	0.0	2.3559351928843197	0.0
10	3.919559441518773E-4	0.0	0.0	2.828242105587332	0.0
11	3.919559441518773E-4	0.0	0.0	3.44467681832562	0.0
12	3.919559441518773E-4	0.0	0.0	3.9256067617999735	0.0
13	3.919559441518773E-4	0.0	0.0	4.1607803282911	0.0
14	3.919559441518773E-4	0.0	0.0	4.293037462589205	0.0
15	3.919559441518773E-4	0.0	0.0	4.398081655621908	0.0
16	3.919559441518773E-4	0.0	0.0	4.552344316498825	0.0
17	3.919559441518773E-4	0.0	0.0	4.739251308152964	0.0
18	3.919559441518773E-4	0.0	0.0	4.941444581629025	0.0
19	3.919559441518773E-4	0.0	0.0	5.07090203061176	0.0
20	4.479496504592884E-4	0.0	0.0	5.195376039733134	0.0
21	4.479496504592884E-4	0.0	0.0	5.331776708297987	0.0
22	4.479496504592884E-4	0.0	0.0	5.472208923716974	0.0
23	4.479496504592884E-4	0.0	0.0	5.617400604172091	0.0
24	5.039433567666994E-4	0.0	0.0	5.7330836014032025	0.0
25	5.039433567666994E-4	0.0	0.0	5.848542623809084	0.0
26	5.039433567666994E-4	0.0	0.0	5.958346281877917	0.0
27	5.039433567666994E-4	0.0	0.0	6.072853411276572	0.0
28	5.599370630741104E-4	0.0	0.0	6.191728049767206	0.0
29	5.599370630741104E-4	0.0	0.0	6.328184712038367	0.0
30	5.599370630741104E-4	0.0	0.0	6.4813274987891365	0.0
31	6.159307693815215E-4	0.0	0.0	6.60820923728173	0.0
32	6.159307693815215E-4	0.0	0.0	6.743993975077201	0.0
33	6.159307693815215E-4	0.0	0.0	6.884706159027726	0.0
34	6.159307693815215E-4	0.0	0.0	7.026426229691783	0.0
35	6.159307693815215E-4	0.0	0.0	7.186232267493134	0.0
36	6.159307693815215E-4	0.0	0.0	7.3318159038924025	0.0
37	6.159307693815215E-4	0.0	0.0	7.488206325609002	0.0
38	6.159307693815215E-4	0.0	0.0	7.661282871805209	0.0
39	6.719244756889325E-4	0.0	0.0	7.86498797535157	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGAATC	20	7.034338E-4	45.0	6
GCGACCG	20	7.034338E-4	45.0	31
CGTTTTT	59620	0.0	44.441463	1
GTTTTTT	67050	0.0	40.03691	2
TATTGCG	40	3.4596633E-7	39.375	1
GACCGAT	720	0.0	37.8125	9
GGACCGA	780	0.0	37.78846	8
CGTTTGG	745	0.0	37.449665	2
CGACCAA	570	0.0	37.105263	29
GGGACCG	890	0.0	36.91011	7
TGGGCGA	2905	0.0	36.712563	6
GCGAGAC	595	0.0	36.68067	21
TTGGGCG	2200	0.0	36.613632	5
GCGTTTG	340	0.0	36.397057	1
TCAAGCG	620	0.0	36.29032	17
CACGACC	590	0.0	36.228813	27
GGGCGAT	4370	0.0	36.092678	7
CTGCGCG	150	0.0	36.000004	1
CGAGACA	600	0.0	36.000004	22
TACGCGG	50	4.881622E-8	36.0	2
>>END_MODULE