FastQCFastQC Report
Sat 14 Jan 2017
SRR2933745.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933745.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1813582
Sequences flagged as poor quality0
Sequence length51
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT927305.113085595247417No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCC27280.15042054894678047No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA22040.12152745230157776No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA19360.10675006699448934No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCG207.034367E-445.01
CTACGCG207.034367E-445.01
CGTTTTT600400.044.456611
GTTTTTT678600.039.877322
ACACGAC5050.037.8712926
GCGAGAC5250.037.71428721
TCAAGCG5250.037.71428717
GTTAGCG301.14018214E-437.4999961
TACGGGA2400.037.4999964
CGACCAA5150.037.13592529
GACCGAT7950.036.7924549
TGGGCGA29550.036.472086
GACACGA5250.036.42857425
TCTTGCG4150.036.3253021
TCTACCG504.881622E-836.0000041
AATTGCG250.002107428636.00000441
ACGTGAG5150.035.82524544
AACACGT5200.035.4807741
GGCACCG7300.035.44528
TTGGGCG21650.035.43885
Produced by FastQC (version 0.11.5)