##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933745.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1813582
Sequences flagged as poor quality	0
Sequence length	51
%GC	34
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.55434052609697	34.0	31.0	34.0	31.0	34.0
2	32.779722670383805	34.0	31.0	34.0	31.0	34.0
3	32.78929764410984	34.0	31.0	34.0	31.0	34.0
4	36.23383888900529	37.0	37.0	37.0	35.0	37.0
5	36.28781273744446	37.0	37.0	37.0	35.0	37.0
6	36.28432957539279	37.0	37.0	37.0	35.0	37.0
7	36.29779739763628	37.0	37.0	37.0	35.0	37.0
8	36.26555181954828	37.0	37.0	37.0	35.0	37.0
9	38.11618002384232	39.0	39.0	39.0	37.0	39.0
10	37.628786567136196	39.0	38.0	39.0	35.0	39.0
11	37.50830841947042	39.0	37.0	39.0	35.0	39.0
12	37.551829473384714	39.0	37.0	39.0	35.0	39.0
13	37.600586573973494	39.0	37.0	39.0	35.0	39.0
14	39.04309703117918	40.0	39.0	41.0	35.0	41.0
15	39.071512619776776	41.0	39.0	41.0	35.0	41.0
16	39.12099921591635	41.0	39.0	41.0	35.0	41.0
17	39.07526431118086	41.0	39.0	41.0	35.0	41.0
18	38.51597611798088	39.0	38.0	41.0	35.0	41.0
19	37.933754856411234	38.0	37.0	41.0	35.0	41.0
20	37.235343645889735	38.0	35.0	41.0	34.0	41.0
21	37.170237684317556	38.0	35.0	41.0	34.0	41.0
22	37.1441010111481	38.0	35.0	41.0	34.0	41.0
23	36.996263196260216	38.0	35.0	40.0	34.0	41.0
24	36.89023435389191	38.0	35.0	40.0	33.0	41.0
25	36.841037240113764	37.0	35.0	40.0	33.0	41.0
26	36.825014253560084	38.0	35.0	40.0	33.0	41.0
27	36.758368797220086	37.0	35.0	40.0	33.0	41.0
28	36.65528109564387	37.0	35.0	40.0	33.0	41.0
29	36.43817594131393	37.0	35.0	40.0	33.0	41.0
30	36.07789225962763	37.0	35.0	40.0	32.0	41.0
31	35.55852781953063	37.0	35.0	40.0	30.0	41.0
32	34.7381552088629	37.0	35.0	40.0	21.0	41.0
33	33.495929050905886	37.0	33.0	40.0	15.0	41.0
34	32.44471934547211	37.0	31.0	40.0	10.0	41.0
35	31.78533035727086	37.0	30.0	40.0	7.0	41.0
36	31.389684613102688	37.0	26.0	40.0	7.0	41.0
37	31.18747043144451	37.0	24.0	40.0	7.0	41.0
38	31.00719515301762	37.0	23.0	40.0	7.0	41.0
39	30.893838271442924	37.0	23.0	40.0	7.0	41.0
40	30.746625187060744	37.0	23.0	40.0	7.0	41.0
41	30.584342478035182	37.0	22.0	40.0	7.0	41.0
42	30.36415888556459	36.0	21.0	40.0	7.0	41.0
43	30.241327935544135	36.0	21.0	40.0	7.0	41.0
44	30.067241514307046	36.0	20.0	40.0	7.0	41.0
45	30.007916929038775	36.0	20.0	40.0	7.0	41.0
46	29.851822525808043	35.0	20.0	40.0	7.0	41.0
47	29.80929729121705	35.0	20.0	40.0	7.0	41.0
48	29.673316122458207	35.0	20.0	40.0	7.0	41.0
49	29.611771069629054	35.0	20.0	40.0	7.0	41.0
50	29.454937797132967	35.0	20.0	40.0	7.0	41.0
51	28.809892797789125	35.0	18.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	7.0
10	13.0
11	7.0
12	10.0
13	13.0
14	31.0
15	50.0
16	124.0
17	327.0
18	767.0
19	1532.0
20	2784.0
21	4575.0
22	7390.0
23	13004.0
24	27018.0
25	58286.0
26	98904.0
27	107613.0
28	88721.0
29	67107.0
30	52963.0
31	48054.0
32	46638.0
33	51558.0
34	72030.0
35	93722.0
36	116489.0
37	144485.0
38	282001.0
39	427150.0
40	208.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	16.581549662491135	3.0482768355662992	38.03373655009809	42.33643695184447
2	46.42012326986042	2.9798487192748935	37.11886200899656	13.481166001868125
3	14.210330715677594	3.2092841680166657	68.87623498689334	13.704150129412401
4	11.963837312015668	3.4760490565080597	68.90545892052303	15.654654710953242
5	11.816559714421516	4.177202905630955	68.84601854231019	15.160218837637338
6	14.639867400536618	4.74944060979873	69.70476107504375	10.9059309146209
7	58.615822168504096	1.24868905844897	37.77474633074214	2.360742442304787
8	61.26301429987726	1.3290273061819096	35.80626627304417	1.6016921208966566
9	57.183132607182905	3.7430345029891123	36.76073097328933	2.3131019165386513
10	23.605218843151288	23.27835190247808	42.73432356518757	10.382105689183065
11	17.22778457218918	19.178785409206753	50.93533129464232	12.65809872396175
12	14.471030259453391	18.422657481161593	53.355017859683215	13.751294399701806
13	14.951956955902737	17.724095188417177	54.92241321318805	12.40153464249204
14	14.956478394690729	18.578591979849822	53.88788596269703	12.57704366276242
15	14.23514348951412	18.702655849032467	54.16920767850585	12.89299298294756
16	15.881167766332045	18.699788595166915	52.99490180206905	12.424141836431989
17	16.096046387756385	18.03844546317729	52.864772588170815	13.00073556089551
18	16.521227052319666	17.144909907575173	53.016792182542616	13.317070857562546
19	16.038921868434954	19.01612389183395	51.66730812281992	13.277646116911173
20	17.297039780941805	19.394325704600067	50.95518151371154	12.353453000746589
21	17.33723647455698	19.5757346510938	51.171879738550565	11.915149135798657
22	17.09412643045641	17.925244074985304	51.37793604038858	13.602693454169703
23	14.94831774907338	20.087429187100444	51.235620997561725	13.728632066264442
24	14.232110817156324	20.2556046542147	51.92083953193184	13.591444996697144
25	14.66787826522319	21.304799011017973	50.706392101377276	13.320930622381564
26	14.985206072843688	21.277504959797792	50.357689919727925	13.3795990476306
27	14.604467843196502	20.236636667104108	51.151864100989094	14.007031388710297
28	14.097680722459751	20.462157211529448	51.21907914833738	14.221082917673423
29	15.008860917234513	19.5932138717742	50.74918035137094	14.648744859620352
30	15.749660064998439	19.57093751481874	50.257942568905065	14.42145985127775
31	15.82597312942012	20.465520720871734	48.98460615511181	14.723899994596328
32	15.639436209666835	21.032740730774787	47.75731122165968	15.570511837898701
33	16.235053060738363	22.599033294331328	44.842030853857175	16.323882791073135
34	15.9976775243689	24.508514089795774	42.65023583163044	16.843572554204883
35	16.42214137546579	25.612847944013563	40.359685969534326	17.605324710986324
36	17.308674214896268	26.933383767593632	38.233010693754125	17.524931323755972
37	17.40864212370877	27.306733304587276	37.748058813993524	17.53656575771043
38	17.47194226674063	27.79923929549367	37.82718399278334	16.90163444498236
39	17.92210112363268	27.55293116054306	37.062895419120835	17.46207229670343
40	18.701056803607447	27.124552405129737	36.98255717138789	17.19183361987492
41	18.053498545971454	27.03726658072257	36.952175308312505	17.95705956499348
42	18.490368783986607	27.568701056803608	36.560795155664316	17.38013500354547
43	18.256963291430992	27.209026115168765	36.764811296097996	17.769199297302247
44	18.326935313650004	27.508213028139888	36.305664701127384	17.859186957082724
45	18.72019020921028	26.714479962858036	36.020979475976276	18.544350351955412
46	18.51093581652222	27.10304800113808	35.89278014448754	18.493236037852164
47	17.802889530222508	27.538815449204947	36.386499204337056	18.271795816235496
48	18.20689662777862	27.075698810420484	36.35468371432888	18.36272084747202
49	18.746877725958903	26.66049839488923	36.14052190637093	18.452101972780937
50	18.14139090485018	26.705437085282057	36.10594944149203	19.047222568375734
51	17.807631527000158	26.360870365938787	35.88296531394776	19.948532793113298
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	527.0
1	2102.0
2	3677.0
3	78340.5
4	153004.0
5	103294.0
6	53584.0
7	52013.5
8	50443.0
9	50505.0
10	50567.0
11	49692.5
12	48818.0
13	47488.0
14	46158.0
15	43973.5
16	41789.0
17	39867.0
18	37945.0
19	35922.0
20	33899.0
21	32045.0
22	30191.0
23	29077.0
24	27963.0
25	28000.5
26	28408.5
27	28779.0
28	30347.5
29	31916.0
30	34880.0
31	37844.0
32	41416.0
33	44988.0
34	49333.5
35	53679.0
36	58685.0
37	63691.0
38	67922.0
39	72153.0
40	75615.0
41	79077.0
42	81752.0
43	84427.0
44	87948.5
45	91470.0
46	95398.0
47	99326.0
48	101920.5
49	104515.0
50	101039.5
51	97564.0
52	90334.5
53	83105.0
54	74690.5
55	66276.0
56	59111.5
57	51947.0
58	45653.5
59	39360.0
60	33618.0
61	27876.0
62	23283.5
63	18691.0
64	15089.5
65	11488.0
66	9084.5
67	6681.0
68	5302.5
69	3924.0
70	3116.5
71	2309.0
72	2060.0
73	1811.0
74	1818.5
75	1528.5
76	1231.0
77	900.5
78	570.0
79	420.5
80	271.0
81	202.5
82	134.0
83	82.0
84	30.0
85	21.0
86	12.0
87	8.0
88	4.0
89	2.5
90	1.0
91	2.0
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1813582.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.021102464286194
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.2218371972047	34.61791877204412
2	9.207065363521465	8.474385969800036
3	4.444992535776198	6.136903708258311
4	2.7574376595884065	5.076012842831982
5	1.8590861308364501	4.277859665857882
6	1.362515857185403	3.7622689123646498
7	1.0668759061561572	3.4369163775723486
8	0.8211611539657802	3.0232593285080527
9	0.6386298519824123	2.645140486935107
>10	2.536037822668062	17.021584739101673
>50	0.045716356743906304	1.4552115482593344
>100	0.03537356497354654	3.3263283381594384
>500	0.0018169996652727587	0.5824434563821722
>1k	0.001332466421200023	0.9943571909924201
>5k	0.0	0.0
>10k+	1.2113331101818391E-4	5.169408662932506
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	92730	5.113085595247417	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCC	2728	0.15042054894678047	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	2204	0.12152745230157776	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	1936	0.10675006699448934	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.3083698448705377E-4	0.0	0.0	0.042953668485902485	0.0
2	3.3083698448705377E-4	0.0	0.0	0.13073574836980076	0.0
3	3.3083698448705377E-4	0.0	0.0	0.21691878282867827	0.0
4	3.3083698448705377E-4	0.0	0.0	0.33100240297929734	0.0
5	3.3083698448705377E-4	0.0	0.0	0.5665583359340797	0.0
6	3.3083698448705377E-4	0.0	0.0	0.9949370913473998	0.0
7	3.3083698448705377E-4	0.0	0.0	1.393650797151714	0.0
8	3.3083698448705377E-4	0.0	0.0	1.924589017756021	0.0
9	3.3083698448705377E-4	0.0	0.0	2.308029082776516	0.0
10	3.3083698448705377E-4	0.0	0.0	2.769491536638542	0.0
11	3.3083698448705377E-4	0.0	0.0	3.378782983068866	0.0
12	3.3083698448705377E-4	0.0	0.0	3.855574217212125	0.0
13	3.3083698448705377E-4	0.0	0.0	4.091019871172079	0.0
14	3.3083698448705377E-4	0.0	0.0	4.2175098782409615	0.0
15	3.8597648190156277E-4	0.0	0.0	4.320675877903508	0.0
16	3.8597648190156277E-4	0.0	0.0	4.467457220020931	0.0
17	3.8597648190156277E-4	0.0	0.0	4.652174536359536	0.0
18	3.8597648190156277E-4	0.0	0.0	4.856245816290634	0.0
19	3.8597648190156277E-4	0.0	0.0	4.982349846877615	0.0
20	3.8597648190156277E-4	0.0	0.0	5.108784714449085	0.0
21	3.8597648190156277E-4	0.0	0.0	5.2406783922645905	0.0
22	4.411159793160717E-4	0.0	0.0	5.377479485349987	0.0
23	4.962554767305807E-4	0.0	0.0	5.517864645767327	0.0
24	4.962554767305807E-4	0.0	0.0	5.637903331638713	0.0
25	6.065344715595986E-4	0.0	0.0	5.749671092897922	0.0
26	6.065344715595986E-4	0.0	0.0	5.856586578384655	0.0
27	6.065344715595986E-4	0.0	0.0	5.973151475918927	0.0
28	6.616739689741075E-4	0.0	0.0	6.090543465914417	0.0
29	6.616739689741075E-4	0.0	0.0	6.221499772273876	0.0
30	6.616739689741075E-4	0.0	0.0	6.364476489069697	0.0
31	7.168134663886166E-4	0.0	0.0	6.495322516434327	0.0
32	7.168134663886166E-4	0.0	0.0	6.633005841478356	0.0
33	7.168134663886166E-4	0.0	0.0	6.767766773159416	0.0
34	7.168134663886166E-4	0.0	0.0	6.902196867855989	0.0
35	7.719529638031255E-4	0.0	0.0	7.0575248320726605	0.0
36	8.270924612176345E-4	0.0	0.0	7.204471592682327	0.0
37	8.270924612176345E-4	0.0	0.0	7.354395886152377	0.0
38	8.270924612176345E-4	0.0	0.0	7.524501235676137	0.0
39	8.270924612176345E-4	0.0	0.0	7.72631179621324	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTGCG	20	7.034367E-4	45.0	1
CTACGCG	20	7.034367E-4	45.0	1
CGTTTTT	60040	0.0	44.45661	1
GTTTTTT	67860	0.0	39.87732	2
ACACGAC	505	0.0	37.87129	26
GCGAGAC	525	0.0	37.714287	21
TCAAGCG	525	0.0	37.714287	17
GTTAGCG	30	1.14018214E-4	37.499996	1
TACGGGA	240	0.0	37.499996	4
CGACCAA	515	0.0	37.135925	29
GACCGAT	795	0.0	36.792454	9
TGGGCGA	2955	0.0	36.47208	6
GACACGA	525	0.0	36.428574	25
TCTTGCG	415	0.0	36.325302	1
TCTACCG	50	4.881622E-8	36.000004	1
AATTGCG	25	0.0021074286	36.000004	41
ACGTGAG	515	0.0	35.825245	44
AACACGT	520	0.0	35.48077	41
GGCACCG	730	0.0	35.4452	8
TTGGGCG	2165	0.0	35.4388	5
>>END_MODULE