FastQCFastQC Report
Sat 14 Jan 2017
SRR2933744.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933744.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1749590
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT815064.65857715236141No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCC47550.2717779594076326No Hit
CTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTGCT26120.14929211986808338TruSeq Adapter, Index 21 (95% over 23bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGC25640.14654861996239119No Hit
CCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTGC22310.12751558936665164No Hit
CGCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTG18900.10802530878663001TruSeq Adapter, Index 21 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG253.891645E-545.01
TATCGCG253.891645E-545.01
CGTTTTT464100.044.5879141
GTTTTTT518900.040.2866672
GCCGATC1350.040.0000049
GTTAGCG451.9292202E-840.01
GAGCGAC1150.039.13043618
CTAGCGG750.039.02
TAGTGCG356.250162E-638.5714261
TACGGGA2650.038.2075464
TTGTACG601.5643309E-1037.5000041
CCGTCGG301.1401655E-437.5000042
GCGATAT601.5643309E-1037.5000049
CGAGCGG301.1401655E-437.5000042
GCGTCCG301.1401655E-437.5000041
CACGACC2250.037.027
TTGGGAC40700.036.76295
TGGGCGA27000.036.6666686
TGCGGGA23000.036.5869564
GCGATCG2100.036.428579
Produced by FastQC (version 0.11.5)