##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933744.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1749590
Sequences flagged as poor quality	0
Sequence length	51
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.39905806503238	33.0	31.0	34.0	31.0	34.0
2	32.6046953857761	34.0	31.0	34.0	31.0	34.0
3	32.66071536760041	34.0	31.0	34.0	31.0	34.0
4	36.16744551580656	37.0	35.0	37.0	35.0	37.0
5	36.183983104613084	37.0	35.0	37.0	35.0	37.0
6	36.16516383838499	37.0	36.0	37.0	35.0	37.0
7	36.25473968186832	37.0	37.0	37.0	35.0	37.0
8	36.1582022073743	37.0	37.0	37.0	35.0	37.0
9	37.98608359672838	39.0	39.0	39.0	35.0	39.0
10	37.432760818248845	39.0	37.0	39.0	34.0	39.0
11	37.340748975474256	39.0	37.0	39.0	34.0	39.0
12	37.41929709246166	39.0	37.0	39.0	34.0	39.0
13	37.447694031173015	39.0	37.0	39.0	35.0	39.0
14	38.734041118204836	40.0	38.0	41.0	35.0	41.0
15	38.771843117530395	40.0	38.0	41.0	35.0	41.0
16	38.790440617516104	40.0	38.0	41.0	35.0	41.0
17	38.73999165518779	40.0	38.0	41.0	34.0	41.0
18	38.2729193696809	39.0	38.0	40.0	34.0	41.0
19	37.80494573014249	38.0	37.0	40.0	34.0	41.0
20	37.2514560554187	38.0	35.0	40.0	34.0	41.0
21	37.146541189650144	38.0	35.0	40.0	33.0	41.0
22	37.15268834412634	38.0	35.0	40.0	33.0	41.0
23	37.06415560216965	38.0	35.0	40.0	33.0	41.0
24	36.97904594790779	38.0	35.0	40.0	33.0	41.0
25	36.91670505661326	38.0	35.0	40.0	33.0	41.0
26	36.86037128698724	38.0	35.0	40.0	33.0	41.0
27	36.79404774832961	38.0	35.0	40.0	33.0	41.0
28	36.654894575300496	38.0	35.0	40.0	33.0	41.0
29	36.53234357763819	38.0	35.0	40.0	33.0	41.0
30	36.30208734617825	38.0	35.0	40.0	32.0	41.0
31	35.851589801039104	38.0	35.0	40.0	30.0	41.0
32	35.14952817517247	38.0	35.0	40.0	24.0	41.0
33	34.26460199246681	38.0	34.0	40.0	18.0	41.0
34	33.49803896912991	38.0	33.0	40.0	15.0	41.0
35	32.89577100920787	38.0	33.0	40.0	10.0	41.0
36	32.51904503340783	38.0	31.0	40.0	9.0	41.0
37	32.309925182471325	38.0	31.0	40.0	8.0	41.0
38	32.17254271000635	38.0	30.0	40.0	8.0	41.0
39	32.09689870198161	38.0	30.0	40.0	8.0	41.0
40	31.945545527809372	37.0	30.0	40.0	8.0	41.0
41	31.690448047828347	37.0	29.0	40.0	7.0	41.0
42	31.509605107482326	37.0	28.0	40.0	7.0	41.0
43	31.42472179196269	37.0	27.0	40.0	7.0	41.0
44	31.321021496464887	37.0	27.0	40.0	7.0	41.0
45	31.308568293142965	37.0	27.0	40.0	7.0	41.0
46	31.126629667522106	36.0	26.0	40.0	7.0	41.0
47	30.963641195937335	36.0	25.0	40.0	7.0	41.0
48	30.84528375219337	36.0	24.0	40.0	7.0	41.0
49	30.829626941169074	36.0	25.0	40.0	7.0	41.0
50	30.722523562663252	36.0	24.0	40.0	7.0	41.0
51	29.81752867814745	35.0	23.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	5.0
10	1.0
11	4.0
12	11.0
13	11.0
14	19.0
15	39.0
16	106.0
17	227.0
18	541.0
19	1227.0
20	2339.0
21	3919.0
22	6309.0
23	10952.0
24	21710.0
25	45765.0
26	74621.0
27	81983.0
28	69153.0
29	55599.0
30	48189.0
31	46707.0
32	49788.0
33	58699.0
34	81091.0
35	106473.0
36	139153.0
37	183758.0
38	315469.0
39	345625.0
40	94.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.412085117084573	3.3555290096536905	42.17782451888728	36.05456135437446
2	41.64724306837602	3.4318897570287894	40.998862590664096	13.922004583931091
3	15.159265885150235	3.3405540726684535	66.31816597031305	15.182014071868267
4	12.941832086374522	3.6409673123417488	66.03341354260141	17.38378705868232
5	12.640904440468908	4.46938997136472	65.41069622025731	17.479009367909054
6	15.619545150578135	5.252373413199664	66.74380854943158	12.384272886790619
7	62.94549008624878	1.219085614343932	32.420795729285146	3.414628570122143
8	64.78186317937346	1.4764030429986454	30.605856229173696	3.1358775484542094
9	59.522973953897775	4.3994307237695685	32.31991495150292	3.7576803708297373
10	24.021227830520292	26.78238901685538	37.58417686429392	11.612206288330409
11	17.799770231882896	19.586131607976725	47.85492601123692	14.759172148903458
12	16.126921164387085	18.300801902159936	49.50319789207757	16.06907904137541
13	16.66927680199361	17.937231008407682	51.523899885115945	13.869592304482763
14	15.515006372921656	19.650318074520314	50.19713189947359	14.637543653084437
15	13.759909464503112	19.9007767534108	51.36986379666093	14.969449985425157
16	15.277750787327316	20.219994398687692	49.46856120576821	15.033693608216783
17	15.46882412450917	19.578872764476248	48.81326482204402	16.139038288970557
18	15.842968924147943	18.791431135294552	49.83270366200081	15.53289627855669
19	15.72248355328963	20.991660903411656	48.358358243931434	14.927497299367282
20	16.671505895666986	22.404391886099027	46.48511937082402	14.438982847409964
21	16.34337187569659	22.76990609228448	47.18088237815717	13.705839653861762
22	15.741516583885367	20.95319474848393	46.76547076743694	16.53981790019376
23	13.635708937522505	22.775450248343898	46.3435433444407	17.2452974696929
24	13.792888619619454	22.313513451722976	47.01850147748901	16.87509645116856
25	14.228362073400053	23.428117444658465	45.673672117467525	16.669848364473964
26	14.593761967089433	23.647368812121698	45.78832755102624	15.97054166976263
27	14.683840213992994	22.55648466212084	46.86292217033705	15.896752953549118
28	14.197097605724771	21.838030624317696	46.949971136094746	17.01490063386279
29	16.005292668568064	20.74680353682863	46.03718585497173	17.21071793963157
30	18.073891597459976	20.927531593116104	45.15840854142971	15.840168267994216
31	17.474894118050514	22.48663972702176	44.10244685897839	15.936019295949336
32	18.040283723615246	22.36152470007259	43.19532004641087	16.40287152990129
33	18.894769631742296	22.46794963391423	41.968975588566465	16.66830514577701
34	19.576243577066627	22.706748438205523	40.48194148343326	17.23506650129459
35	20.799444441269095	23.5938705639607	38.630250515835144	16.976434478935065
36	20.736915505918528	24.52551740693534	39.04777690773267	15.689790179413462
37	20.03212181139581	25.07227407564058	39.58870363913831	15.306900473825296
38	20.3540829565784	24.943958298801434	38.858018164255625	15.843940580364544
39	21.954972307797828	24.327299538749077	36.991237947176195	16.7264902062769
40	22.657937002383417	24.328385507461746	36.63366845946764	16.38000903068719
41	20.18375733743334	24.97893792259901	37.42545396350002	17.41185077646763
42	19.3145251173132	25.408009876599664	38.64808326522214	16.629381740865
43	18.49667636417675	25.203733446121664	39.26759983767625	17.031990352025332
44	19.57430026463343	25.759463645768438	36.17716150641007	18.48907458318806
45	20.810704222132042	24.698243588497874	35.44344675038152	19.047605438988562
46	20.821792534250882	25.179156259466502	35.71791105344681	18.281140152835807
47	18.75222194914237	26.059133854217276	36.940940448905174	18.247703747735184
48	18.31337627672769	25.693962585520037	37.57463177087203	18.41802936688024
49	19.643802262244296	24.50539840762693	36.93036654301865	18.920432787110123
50	18.76862579232849	24.159031544533292	36.41510296698083	20.657239696157387
51	18.213124217673855	23.950811332940862	35.54049805954538	22.295566389839905
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	582.0
1	1671.0
2	2760.0
3	64858.0
4	126956.0
5	83970.5
6	40985.0
7	40156.5
8	39328.0
9	39307.0
10	39286.0
11	39002.0
12	38718.0
13	37508.5
14	36299.0
15	35028.0
16	33757.0
17	31962.0
18	30167.0
19	28808.5
20	27450.0
21	26025.0
22	24600.0
23	23955.5
24	23311.0
25	23323.5
26	23970.5
27	24605.0
28	26375.5
29	28146.0
30	30362.5
31	32579.0
32	35438.0
33	38297.0
34	42545.0
35	46793.0
36	51326.5
37	55860.0
38	58777.0
39	61694.0
40	67784.5
41	73875.0
42	80391.0
43	86907.0
44	95798.0
45	104689.0
46	119409.0
47	134129.0
48	143265.0
49	152401.0
50	144318.0
51	136235.0
52	115266.5
53	94298.0
54	78477.5
55	62657.0
56	52570.5
57	42484.0
58	36635.5
59	30787.0
60	26044.0
61	21301.0
62	18028.5
63	14756.0
64	11371.0
65	7986.0
66	6132.0
67	4278.0
68	3324.0
69	2370.0
70	2030.0
71	1690.0
72	1453.0
73	1216.0
74	1014.0
75	660.0
76	508.0
77	438.5
78	369.0
79	298.0
80	227.0
81	151.0
82	75.0
83	46.5
84	18.0
85	11.5
86	5.0
87	5.0
88	5.0
89	3.5
90	2.0
91	1.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1749590.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.938892785846036
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.02064445236134	31.531399055319888
2	8.709599191346264	7.1312269500443195
3	3.83035205074222	4.704311158121438
4	2.201803968158321	3.6055766635153543
5	1.4299738842677134	2.927077376729786
6	0.9814153555763094	2.4106834812192894
7	0.7926502505320028	2.271515653624256
8	0.6362914467749997	2.0839253856058058
9	0.5338620303375006	1.9670148380178922
>10	3.692609725480947	26.570760538357096
>50	0.10474098329341416	2.945300150658811
>100	0.06087858173426105	4.627395722983526
>500	0.002379117559736413	0.6934195903659283
>1k	0.0026590137432348144	1.8606539384222
>5k	0.0	0.0
>10k+	1.3994809174920075E-4	4.6697394970144055
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	81506	4.65857715236141	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCC	4755	0.2717779594076326	No Hit
CTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTGCT	2612	0.14929211986808338	TruSeq Adapter, Index 21 (95% over 23bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGC	2564	0.14654861996239119	No Hit
CCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTGC	2231	0.12751558936665164	No Hit
CGCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTG	1890	0.10802530878663001	TruSeq Adapter, Index 21 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7146874410576193E-4	0.0	0.0	0.16586743179830704	0.0
2	1.7146874410576193E-4	0.0	0.0	0.5072617013128791	0.0
3	1.7146874410576193E-4	0.0	0.0	0.8187632531050132	0.0
4	1.7146874410576193E-4	0.0	0.0	1.1962802713778657	0.0
5	2.286249921410159E-4	0.0	0.0	2.0148148994907378	0.0
6	2.286249921410159E-4	0.0	0.0	3.2314999514171894	0.0
7	2.286249921410159E-4	0.0	0.0	4.366108631165016	0.0
8	2.286249921410159E-4	0.0	0.0	5.99523316891386	0.0
9	2.286249921410159E-4	0.0	0.0	7.0764579129967595	0.0
10	2.8578124017626986E-4	0.0	0.0	8.4753570836596	0.0
11	2.8578124017626986E-4	0.0	0.0	10.684331757726095	0.0
12	2.8578124017626986E-4	0.0	0.0	12.486353945781584	0.0
13	2.8578124017626986E-4	0.0	0.0	13.320949479592361	0.0
14	2.8578124017626986E-4	0.0	0.0	13.69949531032985	0.0
15	2.8578124017626986E-4	0.0	0.0	14.071582485039352	0.0
16	2.8578124017626986E-4	0.0	0.0	14.747055024319984	0.0
17	2.8578124017626986E-4	0.0	0.0	15.657496899273545	0.0
18	2.8578124017626986E-4	0.0	0.0	16.576112117696145	0.0
19	2.8578124017626986E-4	0.0	0.0	17.140472910796245	0.0
20	2.8578124017626986E-4	0.0	0.0	17.676712829862996	0.0
21	2.8578124017626986E-4	0.0	0.0	18.23667259186438	0.0
22	2.8578124017626986E-4	0.0	0.0	18.769997542281335	0.0
23	2.8578124017626986E-4	0.0	0.0	19.24296549477306	0.0
24	3.4293748821152386E-4	0.0	0.0	19.580015889436954	0.0
25	3.4293748821152386E-4	0.0	0.0	19.86791191079053	0.0
26	3.4293748821152386E-4	0.0	0.0	20.124714933212925	0.0
27	3.4293748821152386E-4	0.0	0.0	20.388148080407408	0.0
28	3.4293748821152386E-4	0.0	0.0	20.64066438422716	0.0
29	3.4293748821152386E-4	0.0	0.0	20.915585937276735	0.0
30	4.000937362467778E-4	0.0	0.0	21.205539583559577	0.0
31	4.000937362467778E-4	0.0	0.0	21.463257105950536	0.0
32	4.000937362467778E-4	0.0	0.0	21.73669259655119	0.0
33	4.000937362467778E-4	0.0	0.0	21.986179619225076	0.0
34	4.000937362467778E-4	0.0	0.0	22.24429723535228	0.0
35	4.000937362467778E-4	0.0	0.0	22.511274069924955	0.0
36	4.000937362467778E-4	0.0	0.0	22.779679810698507	0.0
37	4.000937362467778E-4	0.0	0.0	23.046370864031	0.0
38	4.000937362467778E-4	0.0	0.0	23.318891854663093	0.0
39	4.572499842820318E-4	0.0	0.0	23.651598374476308	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCGACG	25	3.891645E-5	45.0	1
TATCGCG	25	3.891645E-5	45.0	1
CGTTTTT	46410	0.0	44.587914	1
GTTTTTT	51890	0.0	40.286667	2
GCCGATC	135	0.0	40.000004	9
GTTAGCG	45	1.9292202E-8	40.0	1
GAGCGAC	115	0.0	39.130436	18
CTAGCGG	75	0.0	39.0	2
TAGTGCG	35	6.250162E-6	38.571426	1
TACGGGA	265	0.0	38.207546	4
TTGTACG	60	1.5643309E-10	37.500004	1
CCGTCGG	30	1.1401655E-4	37.500004	2
GCGATAT	60	1.5643309E-10	37.500004	9
CGAGCGG	30	1.1401655E-4	37.500004	2
GCGTCCG	30	1.1401655E-4	37.500004	1
CACGACC	225	0.0	37.0	27
TTGGGAC	4070	0.0	36.7629	5
TGGGCGA	2700	0.0	36.666668	6
TGCGGGA	2300	0.0	36.586956	4
GCGATCG	210	0.0	36.42857	9
>>END_MODULE