FastQCFastQC Report
Sat 14 Jan 2017
SRR2933743.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933743.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1766427
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT810974.591019045791307No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCC48170.2726973715868247No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGC27310.15460587955233926No Hit
CTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTGCT27140.15364348484256637TruSeq Adapter, Index 21 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTGC23310.13196129814591828No Hit
CGCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTG18780.10631630970314651TruSeq Adapter, Index 21 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGAC207.0343155E-445.0000049
GTTCGAC207.0343155E-445.00000415
CAGTCGT207.0343155E-445.00000425
TTCGACG207.0343155E-445.0000041
GTTAGCG351.2124292E-745.01
GAACGCG253.8916594E-545.031
CGTTTTT456000.044.5263141
AGCTACG501.0822987E-940.59
GTTTTTT514900.039.7562642
CTAGCGG659.094947E-1238.076922
GAGCGAC601.5643309E-1037.50000418
CACGACC2300.037.17391627
GCGATCG2250.037.09
GGACCGA7300.036.678088
TCAAGCG2650.036.50943417
TTGGGAC41200.036.4259725
TGGGCGA28100.036.2722436
GACCGAT7050.036.0638289
CGAGTGA250.002107414236.022
AACCCGC250.002107414236.019
Produced by FastQC (version 0.11.5)