##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933743.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1766427
Sequences flagged as poor quality	0
Sequence length	51
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.31976470015461	33.0	31.0	34.0	30.0	34.0
2	32.53924164429099	34.0	31.0	34.0	31.0	34.0
3	32.604523708027564	34.0	31.0	34.0	31.0	34.0
4	36.08592429803213	37.0	35.0	37.0	35.0	37.0
5	36.15325796084412	37.0	35.0	37.0	35.0	37.0
6	36.15356196434951	37.0	36.0	37.0	35.0	37.0
7	36.17734953100241	37.0	37.0	37.0	35.0	37.0
8	36.11833039236832	37.0	37.0	37.0	35.0	37.0
9	37.9500873797785	39.0	38.0	39.0	35.0	39.0
10	37.44795624161089	39.0	37.0	39.0	34.0	39.0
11	37.3443827568306	39.0	37.0	39.0	34.0	39.0
12	37.399829712747824	39.0	37.0	39.0	34.0	39.0
13	37.438973136166965	39.0	37.0	39.0	35.0	39.0
14	38.75482541876907	40.0	38.0	41.0	35.0	41.0
15	38.777056170450294	40.0	38.0	41.0	35.0	41.0
16	38.852254296384736	40.0	38.0	41.0	35.0	41.0
17	38.82925249670663	40.0	38.0	41.0	35.0	41.0
18	38.408011200009966	39.0	38.0	41.0	35.0	41.0
19	37.977219551105144	39.0	37.0	41.0	35.0	41.0
20	37.37880818171371	39.0	35.0	41.0	34.0	41.0
21	37.30826181891468	38.0	35.0	40.0	34.0	41.0
22	37.28780017515584	38.0	35.0	40.0	34.0	41.0
23	37.13806910786577	38.0	35.0	40.0	33.0	41.0
24	37.063695244694514	38.0	35.0	40.0	33.0	41.0
25	37.01023421856663	38.0	35.0	40.0	33.0	41.0
26	36.995125187737735	38.0	35.0	40.0	33.0	41.0
27	36.929319468056136	38.0	35.0	40.0	33.0	41.0
28	36.85115037304117	38.0	35.0	40.0	33.0	41.0
29	36.668373501990175	38.0	35.0	40.0	33.0	41.0
30	36.35772267973712	38.0	35.0	40.0	32.0	41.0
31	35.9012566044337	38.0	35.0	40.0	30.0	41.0
32	35.284005509426656	38.0	35.0	40.0	25.0	41.0
33	34.335377572919796	38.0	34.0	40.0	18.0	41.0
34	33.58299040945366	38.0	33.0	40.0	15.0	41.0
35	33.03682575051219	38.0	33.0	40.0	10.0	41.0
36	32.66656929496662	38.0	31.0	40.0	10.0	41.0
37	32.49583594453663	38.0	31.0	40.0	8.0	41.0
38	32.33554570893674	38.0	31.0	40.0	8.0	41.0
39	32.30249028122872	38.0	31.0	40.0	8.0	41.0
40	32.147175626278354	38.0	30.0	40.0	8.0	41.0
41	31.92759055426576	37.0	30.0	40.0	8.0	41.0
42	31.68670712121135	37.0	29.0	40.0	7.0	41.0
43	31.625253123961535	37.0	28.0	40.0	7.0	41.0
44	31.542747591607238	37.0	28.0	40.0	7.0	41.0
45	31.502984838886633	37.0	27.0	40.0	7.0	41.0
46	31.3069467348495	37.0	26.0	40.0	7.0	41.0
47	31.23479260677062	37.0	26.0	40.0	7.0	41.0
48	31.073810013094228	36.0	26.0	40.0	7.0	41.0
49	31.04378046757664	36.0	26.0	40.0	7.0	41.0
50	30.86679324987673	36.0	26.0	40.0	7.0	41.0
51	30.093787062810975	35.0	24.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	6.0
10	4.0
11	8.0
12	15.0
13	10.0
14	24.0
15	31.0
16	113.0
17	245.0
18	592.0
19	1271.0
20	2314.0
21	3891.0
22	6206.0
23	10781.0
24	21855.0
25	44772.0
26	74532.0
27	80437.0
28	68811.0
29	55142.0
30	47933.0
31	46560.0
32	48723.0
33	57281.0
34	79806.0
35	104123.0
36	136625.0
37	177653.0
38	317825.0
39	378650.0
40	186.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.586955475657923	3.528139006027421	42.0726132469669	35.81229227134776
2	41.62187285407209	3.4644511208218627	40.9451395387412	13.96853648636485
3	15.327777485285269	3.417463614403539	66.05401751671594	15.200741383595245
4	13.162219553935714	3.708446485476048	65.69362900363276	17.43570495695548
5	12.709158091446746	4.5360493244272195	65.32673017339522	17.428062410730814
6	15.619779362521067	5.309871282538141	66.61367834617565	12.45667100876515
7	63.02173823203563	1.2767014996940151	32.24367607605635	3.4578841922140002
8	64.91318350546045	1.48916428473976	30.455999596926453	3.1416526128733318
9	59.678888513366246	4.426223104606078	32.10775197616431	3.787136405863362
10	24.09123048957019	26.83331946352722	37.44598559691399	11.629464449988593
11	17.894370953342538	19.662120200834792	47.7194925122861	14.724016333536568
12	16.192743883557036	18.421253751216437	49.35652591360979	16.029476451616738
13	16.7318547553904	18.019312431252466	51.39006593535991	13.858766877997223
14	15.58632199349308	19.721618838480165	49.99504649781735	14.69701267020941
15	13.801815755760074	20.022508713917983	51.24055508662402	14.935120443697928
16	15.38710628857009	20.274599516424967	49.24658647088162	15.09170772412333
17	15.546863810392392	19.6307574555869	48.753953602384925	16.068425131635784
18	15.929783681974971	18.905168455871653	49.58325478494158	15.581793077211795
19	15.730567977051981	21.093031299906535	48.18834857030605	14.988052152735436
20	16.76701046802387	22.500335422862083	46.35526970545627	14.377384403657778
21	16.435776853501448	22.844589671693196	47.075367394180454	13.6442660806249
22	15.844696667340344	20.981280290665847	46.669519883923876	16.504503158069934
23	13.791625694127186	22.865139629319525	46.226535260160766	17.116699416392525
24	13.861540839219511	22.409643874329365	46.833976156388005	16.894839130063115
25	14.252669371561916	23.65339750807704	45.479094239388324	16.61483888097272
26	14.690785410322645	23.654359902786812	45.66676120779404	15.988093479096504
27	14.75826626291378	22.67917100451929	46.64398811838814	15.918574614178791
28	14.292806892104798	21.858191705629498	46.886738031064965	16.962263371200734
29	16.16783484400997	20.81161576447824	45.907642942504836	17.11290644900695
30	18.125175849327483	21.007378170736747	45.06226410714963	15.80518187278614
31	17.605426094596606	22.55219151428279	43.92312843949962	15.919253951620984
32	18.09726640274407	22.4742941542447	43.08624132217182	16.342198120839413
33	18.90528167877869	22.5219043866517	41.817805094691145	16.755008839878467
34	19.66546027659224	22.832304986280214	40.404783214930475	17.09745152219707
35	20.72477379478461	23.692063130828505	38.577761775606916	17.005401298779965
36	20.74560680967852	24.611489747382713	38.9786840894076	15.664219353531166
37	19.963349744993707	25.171037353935372	39.59489976092983	15.270713140141087
38	20.36444189315494	25.004826126412244	38.8453641163773	15.78536786405552
39	21.871948288833902	24.439221094333362	37.070708271556086	16.61812234527665
40	22.64016571304673	24.406103394026474	36.711282153182665	16.242448739744127
41	20.188097215452437	25.13180561664875	37.42690753707908	17.25318963081973
42	19.31260108682668	25.47538052803767	38.69658921653711	16.515429168598533
43	18.555536118956514	25.319189527786882	39.14534820855886	16.979926144697743
44	19.553369598630454	25.8570549476429	36.203873695318286	18.385701758408356
45	20.786083998942498	24.871562764835456	35.30805405488028	19.034299181341773
46	20.803520326625442	25.393746812067526	35.56886302122873	18.233869840078302
47	18.672268936106615	26.180476181580104	36.94106804300433	18.206186839308955
48	18.446672293845147	25.88836108143727	37.35970974175553	18.30525688296205
49	19.55721917746955	24.66838425816634	36.956919249988815	18.81747731437529
50	18.839385946886004	24.308448636711283	36.30379291077412	20.548372505628592
51	18.17929639888883	24.084437115148262	35.59196049426328	22.14430599169963
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	569.0
1	1787.0
2	3005.0
3	65240.0
4	127475.0
5	84786.5
6	42098.0
7	40842.5
8	39587.0
9	39648.0
10	39709.0
11	39235.5
12	38762.0
13	37611.0
14	36460.0
15	35160.5
16	33861.0
17	32055.5
18	30250.0
19	28594.0
20	26938.0
21	25582.5
22	24227.0
23	23902.5
24	23578.0
25	23539.0
26	24258.0
27	25016.0
28	26718.0
29	28420.0
30	30589.5
31	32759.0
32	35965.5
33	39172.0
34	43679.0
35	48186.0
36	52449.5
37	56713.0
38	59804.0
39	62895.0
40	69304.0
41	75713.0
42	81774.0
43	87835.0
44	96666.0
45	105497.0
46	120282.5
47	135068.0
48	144134.0
49	153200.0
50	145055.5
51	136911.0
52	116100.5
53	95290.0
54	79298.5
55	63307.0
56	53368.5
57	43430.0
58	37156.0
59	30882.0
60	26087.0
61	21292.0
62	18015.5
63	14739.0
64	11489.0
65	8239.0
66	6344.5
67	4450.0
68	3476.5
69	2503.0
70	2095.0
71	1687.0
72	1477.5
73	1268.0
74	1042.5
75	645.0
76	473.0
77	411.5
78	350.0
79	270.0
80	190.0
81	132.0
82	74.0
83	46.0
84	18.0
85	12.5
86	7.0
87	5.0
88	3.0
89	3.0
90	3.0
91	2.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1766427.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.64772944380871
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.86747554386444	31.24488348935584
2	8.811049424413426	7.1629830623916675
3	3.773824915379509	4.601922423859516
4	2.1323480095147693	3.4670041988320155
5	1.4276377705685883	2.9015116920917112
6	1.078934532102161	2.631374336908259
7	0.7842792330109613	2.2315419050279157
8	0.6339049699816852	2.061343817032098
9	0.5334715371231192	1.951596603625798
>10	3.7845044894769373	26.926594075700745
>50	0.10416031347920353	2.8990371337319623
>100	0.06349288389585989	4.793928200811123
>500	0.002247486715214947	0.6725457980544469
>1k	0.0025284225546168154	1.8233362352551752
>5k	0.0	0.0
>10k+	1.404679197009342E-4	4.630397027321631
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	81097	4.591019045791307	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCC	4817	0.2726973715868247	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGC	2731	0.15460587955233926	No Hit
CTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTGCT	2714	0.15364348484256637	TruSeq Adapter, Index 21 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTGC	2331	0.13196129814591828	No Hit
CGCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTG	1878	0.10631630970314651	TruSeq Adapter, Index 21 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.830572675802623E-4	0.0	0.0	0.16558850153445345	0.0
2	2.830572675802623E-4	0.0	0.0	0.4967088931498443	0.0
3	2.830572675802623E-4	0.0	0.0	0.8021842963224634	0.0
4	3.3966872109631477E-4	0.0	0.0	1.1844248304628495	0.0
5	3.3966872109631477E-4	0.0	0.0	2.0053475178991262	0.0
6	3.3966872109631477E-4	0.0	0.0	3.1831488083005977	0.0
7	3.3966872109631477E-4	0.0	0.0	4.305980377337982	0.0
8	3.3966872109631477E-4	0.0	0.0	5.887704388576488	0.0
9	3.3966872109631477E-4	0.0	0.0	6.9443005570000915	0.0
10	3.3966872109631477E-4	0.0	0.0	8.347075763674354	0.0
11	3.3966872109631477E-4	0.0	0.0	10.491177954141326	0.0
12	3.3966872109631477E-4	0.0	0.0	12.289837055253345	0.0
13	3.3966872109631477E-4	0.0	0.0	13.106230826408337	0.0
14	3.962801746123672E-4	0.0	0.0	13.485187896244792	0.0
15	3.962801746123672E-4	0.0	0.0	13.841670219035375	0.0
16	4.528916281284197E-4	0.0	0.0	14.506232071860314	0.0
17	5.095030816444721E-4	0.0	0.0	15.37952035379894	0.0
18	5.095030816444721E-4	0.0	0.0	16.266168938767354	0.0
19	5.095030816444721E-4	0.0	0.0	16.816885158571512	0.0
20	5.095030816444721E-4	0.0	0.0	17.334200620801198	0.0
21	5.095030816444721E-4	0.0	0.0	17.878632969265077	0.0
22	5.095030816444721E-4	0.0	0.0	18.399458341612757	0.0
23	5.095030816444721E-4	0.0	0.0	18.859539624337717	0.0
24	5.661145351605246E-4	0.0	0.0	19.189414563975756	0.0
25	5.661145351605246E-4	0.0	0.0	19.46567845713409	0.0
26	6.227259886765771E-4	0.0	0.0	19.725808086040352	0.0
27	6.227259886765771E-4	0.0	0.0	19.97908772907117	0.0
28	6.793374421926295E-4	0.0	0.0	20.226876061110932	0.0
29	6.793374421926295E-4	0.0	0.0	20.49510112786999	0.0
30	6.793374421926295E-4	0.0	0.0	20.77776211527564	0.0
31	6.793374421926295E-4	0.0	0.0	21.04479834151086	0.0
32	6.793374421926295E-4	0.0	0.0	21.308834160709726	0.0
33	6.793374421926295E-4	0.0	0.0	21.55803777908739	0.0
34	6.793374421926295E-4	0.0	0.0	21.812506262642046	0.0
35	6.793374421926295E-4	0.0	0.0	22.082259838646035	0.0
36	6.793374421926295E-4	0.0	0.0	22.34963573360235	0.0
37	7.35948895708682E-4	0.0	0.0	22.614407501696927	0.0
38	7.35948895708682E-4	0.0	0.0	22.878443320895798	0.0
39	7.35948895708682E-4	0.0	0.0	23.213866182978407	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACCGAC	20	7.0343155E-4	45.000004	9
GTTCGAC	20	7.0343155E-4	45.000004	15
CAGTCGT	20	7.0343155E-4	45.000004	25
TTCGACG	20	7.0343155E-4	45.000004	1
GTTAGCG	35	1.2124292E-7	45.0	1
GAACGCG	25	3.8916594E-5	45.0	31
CGTTTTT	45600	0.0	44.526314	1
AGCTACG	50	1.0822987E-9	40.5	9
GTTTTTT	51490	0.0	39.756264	2
CTAGCGG	65	9.094947E-12	38.07692	2
GAGCGAC	60	1.5643309E-10	37.500004	18
CACGACC	230	0.0	37.173916	27
GCGATCG	225	0.0	37.0	9
GGACCGA	730	0.0	36.67808	8
TCAAGCG	265	0.0	36.509434	17
TTGGGAC	4120	0.0	36.425972	5
TGGGCGA	2810	0.0	36.272243	6
GACCGAT	705	0.0	36.063828	9
CGAGTGA	25	0.0021074142	36.0	22
AACCCGC	25	0.0021074142	36.0	19
>>END_MODULE