FastQCFastQC Report
Sat 14 Jan 2017
SRR2933742.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933742.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1980623
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT968494.88982507019256No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCC34340.17337979009634846No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA20800.10501746167746208No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGA207.034525E-445.022
CGTTTTT536350.044.3455771
TACGAAT1200.041.25000412
GTTTTTT605650.039.8844222
CGTTTGG10100.037.871292
CGAATAT1250.037.80000314
GCTACGA1350.036.66666810
TGGGCGA32850.036.575346
TTGGGAC42600.036.232395
TACGGGA4000.036.04
CGACCAA9050.035.55248629
TCAAGCG9650.035.44041417
TCGTTTG5400.035.4166681
GGGCGAT53150.035.3904047
ACTTGCG1150.035.2173921
CACGACC9150.035.16393727
GACACGA9100.035.1098925
CGAGACA9500.035.0526322
TTGGGAT67950.034.602655
ACACGAC9250.034.5405426
Produced by FastQC (version 0.11.5)