##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933742.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1980623
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.598464220601294	34.0	31.0	34.0	31.0	34.0
2	32.793766405822815	34.0	31.0	34.0	31.0	34.0
3	32.8257820897768	34.0	31.0	34.0	31.0	34.0
4	36.27959889388339	37.0	37.0	37.0	35.0	37.0
5	36.28483663978455	37.0	37.0	37.0	35.0	37.0
6	36.25914472365513	37.0	37.0	37.0	35.0	37.0
7	36.33369752850492	37.0	37.0	37.0	35.0	37.0
8	36.255374697759244	37.0	37.0	37.0	35.0	37.0
9	38.103173597398396	39.0	39.0	39.0	37.0	39.0
10	37.59971332252529	39.0	38.0	39.0	35.0	39.0
11	37.48951617748557	39.0	37.0	39.0	35.0	39.0
12	37.509314998361624	39.0	37.0	39.0	35.0	39.0
13	37.532660683027515	39.0	37.0	39.0	35.0	39.0
14	38.88411626038878	40.0	38.0	41.0	35.0	41.0
15	38.93097373907099	40.0	38.0	41.0	35.0	41.0
16	38.951009354127464	40.0	38.0	41.0	35.0	41.0
17	38.8901497155188	40.0	38.0	41.0	35.0	41.0
18	38.40689015526932	39.0	38.0	41.0	35.0	41.0
19	37.92470550932712	39.0	37.0	41.0	35.0	41.0
20	37.379494734737506	38.0	35.0	41.0	34.0	41.0
21	37.27975742985919	38.0	35.0	41.0	34.0	41.0
22	37.27272327949337	38.0	35.0	41.0	34.0	41.0
23	37.182050294276095	38.0	35.0	41.0	34.0	41.0
24	37.09908750933418	38.0	35.0	41.0	33.0	41.0
25	37.03254783974538	38.0	35.0	40.0	33.0	41.0
26	36.98568076812195	38.0	35.0	40.0	33.0	41.0
27	36.90883121118961	38.0	35.0	40.0	33.0	41.0
28	36.75892029931996	38.0	35.0	40.0	33.0	41.0
29	36.609211848999024	38.0	35.0	40.0	33.0	41.0
30	36.382646773262756	38.0	35.0	40.0	33.0	41.0
31	35.95288502658002	38.0	35.0	40.0	31.0	41.0
32	35.224706064707924	38.0	35.0	40.0	25.0	41.0
33	34.33718077594777	38.0	34.0	40.0	18.0	41.0
34	33.513119861780865	38.0	33.0	40.0	14.0	41.0
35	32.963177747607695	38.0	33.0	40.0	10.0	41.0
36	32.64975717236445	38.0	32.0	40.0	9.0	41.0
37	32.455457197053654	38.0	31.0	40.0	8.0	41.0
38	32.29442806632055	37.0	31.0	40.0	8.0	41.0
39	32.182586489200624	37.0	31.0	40.0	8.0	41.0
40	32.02757667663155	37.0	30.0	40.0	8.0	41.0
41	31.85964517225136	37.0	30.0	40.0	7.0	41.0
42	31.700374579109706	37.0	29.0	40.0	7.0	41.0
43	31.55608008187323	37.0	29.0	40.0	7.0	41.0
44	31.33600942733675	37.0	27.0	40.0	7.0	41.0
45	31.33609929804915	36.0	27.0	40.0	7.0	41.0
46	31.239607436649983	36.0	26.0	40.0	7.0	41.0
47	31.162321148446726	36.0	26.0	40.0	7.0	41.0
48	31.081843440170086	36.0	26.0	40.0	7.0	41.0
49	31.051551961175853	36.0	26.0	40.0	7.0	41.0
50	30.94988546533086	36.0	26.0	40.0	7.0	41.0
51	30.146321637181835	35.0	24.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	2.0
9	4.0
10	7.0
11	8.0
12	14.0
13	27.0
14	30.0
15	54.0
16	131.0
17	287.0
18	676.0
19	1394.0
20	2492.0
21	3977.0
22	6685.0
23	11672.0
24	23299.0
25	49386.0
26	83110.0
27	93911.0
28	81058.0
29	63902.0
30	53383.0
31	50318.0
32	51595.0
33	61279.0
34	90271.0
35	115947.0
36	149011.0
37	185476.0
38	341842.0
39	459223.0
40	150.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.011088935148184	3.173748865887148	39.66862951707619	38.14653268188848
2	42.546966282831214	3.323701683763139	40.09172871364212	14.037603319763528
3	16.14269853475396	3.5792273441235407	65.94046418727845	14.337609933844048
4	13.408255887162776	3.7143363477047373	65.94071663309978	16.9366911320327
5	13.325302190270435	4.529584883140305	65.03150776296145	17.113605163627806
6	16.154109085878535	5.2844988672756	66.134039643082	12.427352403763866
7	63.69879578294304	1.4275306305137323	32.20269581843693	2.670977768106298
8	65.7811203848486	1.6915384704711596	30.468292047502228	2.059049097178009
9	61.13182569322885	4.150966640294493	31.714061686651117	3.0031459798255398
10	25.432805738396453	25.912503288106826	37.529100692054975	11.125590281441749
11	19.653260615473013	19.548798534602497	46.742060452695945	14.055880397228549
12	16.496526597944182	19.438479710676894	48.77737964266799	15.287614048710935
13	17.079171553597025	18.366089861624346	50.533847178387816	14.020891406390817
14	16.31633077067165	19.422474645603934	49.75166904554779	14.509525538176623
15	15.451905789239042	19.217438149511544	50.26645656442442	15.06419949682499
16	17.32596258853906	19.50552932082481	48.88184172353851	14.286666367097625
17	17.329345362545016	19.247984093893688	48.644492162314585	14.77817838124671
18	18.059721612846058	18.002012498087723	48.77445127114044	15.163814617925775
19	17.584972001233957	19.791853371388697	47.35954293169372	15.26363169568363
20	19.02588226027871	20.608515603423772	46.42382725031467	13.941774885982843
21	18.947523077334758	20.97834873168695	46.52187720732315	13.552250983655142
22	18.60561045691179	18.849624587819086	46.9893058901164	15.555459065152732
23	16.114323624435343	21.34596033672233	46.83925209391187	15.70046394493046
24	15.507898272412266	21.038329858837347	47.84908586843635	15.604686000314041
25	16.306687340296463	22.242092513315253	46.08625669801876	15.36496344836953
26	16.45754896312928	21.94400448747692	45.995426691500604	15.6030198578932
27	16.121896999075545	21.114719964374846	46.693237430848775	16.070145605700834
28	15.403385702377484	21.61304801570011	46.838949158926255	16.14461712299615
29	16.486024851776435	20.931949189724648	46.14744956511158	16.434576393387335
30	17.563615084748587	20.7535204832015	46.173855398023754	15.509009034026164
31	18.051239433249034	21.712208734322484	44.394213335904915	15.84233849652357
32	17.74017569219382	22.249110507148508	43.83570220077218	16.17501159988549
33	18.682555943256236	23.03305576073791	41.19840070523264	17.085987590773204
34	18.17614962564809	24.753877946484515	40.38022379826953	16.689748629597858
35	19.102423833309015	25.831619646949473	38.29401153071533	16.77194498902618
36	19.795236145394657	26.888256876750393	36.708500305207	16.60800667264795
37	20.49415764635673	26.279610001499528	36.2990836721577	16.927148679986047
38	20.0398056571089	26.819490635017367	36.646095698171735	16.494608009702
39	20.424331132174068	26.376599686058377	35.406384758735	17.79268442303255
40	21.136329326681555	26.344942980062335	35.592689774883965	16.92603791837215
41	19.744847959455182	26.491614002260906	35.614703050504815	18.148834987779097
42	20.503801076731918	26.518979129294166	35.50751455476383	17.469705239210086
43	19.951096195490006	26.12385092973272	35.78404370745972	18.141009167317556
44	19.95528679612425	26.713564368383082	35.14697143272596	18.184177402766704
45	20.10029167590198	25.95516663191329	34.86428260198937	19.08025909019536
46	19.75252231242392	26.440115054707537	34.9668260946177	18.840536538250845
47	18.98458212390748	26.972321335256634	35.87517664896348	18.167919891872405
48	19.36562384663815	26.425978088712494	35.81681117506966	18.391586889579692
49	20.113014945297515	25.742354804523625	35.53780805332464	18.60682219685422
50	19.069504898206272	26.153589047486577	35.65878009091079	19.118125963396366
51	18.857500897444897	25.198334059535814	35.41925949562334	20.524905547395946
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	941.0
1	2515.0
2	4089.0
3	80035.5
4	155982.0
5	102191.5
6	48401.0
7	47219.5
8	46038.0
9	46112.0
10	46186.0
11	45399.5
12	44613.0
13	42897.5
14	41182.0
15	39223.0
16	37264.0
17	34879.5
18	32495.0
19	30454.0
20	28413.0
21	26938.0
22	25463.0
23	24931.0
24	24399.0
25	24719.0
26	26280.0
27	27521.0
28	29015.0
29	30509.0
30	34181.0
31	37853.0
32	41828.5
33	45804.0
34	50911.5
35	56019.0
36	60254.0
37	64489.0
38	68536.0
39	72583.0
40	76766.5
41	80950.0
42	86188.5
43	91427.0
44	97482.5
45	103538.0
46	111096.0
47	118654.0
48	122511.5
49	126369.0
50	124209.5
51	122050.0
52	113097.0
53	104144.0
54	94299.5
55	84455.0
56	75908.0
57	67361.0
58	59668.0
59	51975.0
60	46304.0
61	40633.0
62	35938.5
63	31244.0
64	26500.5
65	21757.0
66	17802.0
67	13847.0
68	11280.5
69	8714.0
70	7173.0
71	5632.0
72	4954.5
73	4277.0
74	3979.5
75	3104.5
76	2527.0
77	1931.5
78	1336.0
79	897.0
80	458.0
81	337.5
82	217.0
83	129.0
84	41.0
85	33.0
86	25.0
87	17.0
88	9.0
89	11.5
90	14.0
91	8.0
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1980623.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.69884362652323
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.51173261357106	28.090061561403264
2	7.939166012456829	5.9859475605723595
3	3.830014542753753	4.331613580037508
4	2.4629838374667052	3.714065701732457
5	1.7308610833892175	3.2625730660962344
6	1.3923127145784546	3.1493147583667938
7	1.1283629716383563	2.9776585455227425
8	0.9557447342274727	2.88243770260116
9	0.8305927837007444	2.818114872304769
>10	5.097336083489846	30.991891176413176
>50	0.06916379810919432	1.836652748386109
>100	0.04675800463428363	3.2471084323063106
>500	0.002955622693213803	0.7300775384930607
>1k	0.0018808508047724198	1.0773645091102593
>5k	0.0	0.0
>10k+	1.3434648605517284E-4	4.905118246653793
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	96849	4.88982507019256	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCC	3434	0.17337979009634846	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	2080	0.10501746167746208	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5146749280403187E-4	0.0	0.0	0.0627580311851372	0.0
2	1.5146749280403187E-4	0.0	0.0	0.17757039073059336	0.0
3	1.5146749280403187E-4	0.0	0.0	0.2989968307951589	0.0
4	1.5146749280403187E-4	0.0	0.0	0.4464756796220179	0.0
5	1.5146749280403187E-4	0.0	0.0	0.7838442752608649	0.0
6	1.5146749280403187E-4	0.0	0.0	1.3777483145454739	0.0
7	1.5146749280403187E-4	0.0	0.0	1.9070262235670292	0.0
8	1.5146749280403187E-4	0.0	0.0	2.680722176810024	0.0
9	1.5146749280403187E-4	0.0	0.0	3.232518253095112	0.0
10	1.5146749280403187E-4	0.0	0.0	3.9200796920968806	0.0
11	1.5146749280403187E-4	0.0	0.0	4.758502753931465	0.0
12	1.5146749280403187E-4	0.0	0.0	5.430362062845882	0.0
13	1.5146749280403187E-4	0.0	0.0	5.750261407647997	0.0
14	1.5146749280403187E-4	0.0	0.0	5.927579352557251	0.0
15	1.5146749280403187E-4	0.0	0.0	6.074502820577162	0.0
16	2.019566570720425E-4	0.0	0.0	6.297059056670553	0.0
17	2.019566570720425E-4	0.0	0.0	6.566166302219049	0.0
18	2.524458213400531E-4	0.0	0.0	6.852540841947206	0.0
19	2.524458213400531E-4	0.0	0.0	7.04631825440783	0.0
20	2.524458213400531E-4	0.0	0.0	7.237722676147858	0.0
21	2.524458213400531E-4	0.0	0.0	7.444829227975238	0.0
22	2.524458213400531E-4	0.0	0.0	7.6659212783048565	0.0
23	2.524458213400531E-4	0.0	0.0	7.867978913705435	0.0
24	3.0293498560806373E-4	0.0	0.0	8.03232114339781	0.0
25	3.5342414987607436E-4	0.0	0.0	8.182425428766605	0.0
26	3.5342414987607436E-4	0.0	0.0	8.328389602665425	0.0
27	3.5342414987607436E-4	0.0	0.0	8.484451609417844	0.0
28	4.03913314144085E-4	0.0	0.0	8.643542966026347	0.0
29	4.03913314144085E-4	0.0	0.0	8.8195986818289	0.0
30	4.03913314144085E-4	0.0	0.0	9.03609621821013	0.0
31	5.048916426801062E-4	1.0097832853602124E-4	0.0	9.19796447885337	0.0
32	5.048916426801062E-4	1.0097832853602124E-4	0.0	9.405222498173554	0.0
33	5.048916426801062E-4	1.0097832853602124E-4	0.0	9.582035551440129	0.0
34	5.048916426801062E-4	1.0097832853602124E-4	0.0	9.753850177444168	0.0
35	5.048916426801062E-4	1.0097832853602124E-4	0.0	9.955150475380727	0.0
36	5.553808069481168E-4	1.0097832853602124E-4	0.0	10.124541621499901	0.0
37	5.553808069481168E-4	1.0097832853602124E-4	0.0	10.29766896577491	0.0
38	6.058699712161275E-4	1.0097832853602124E-4	0.0	10.503866712645465	0.0
39	6.058699712161275E-4	1.0097832853602124E-4	0.0	10.79104907900191	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGA	20	7.034525E-4	45.0	22
CGTTTTT	53635	0.0	44.345577	1
TACGAAT	120	0.0	41.250004	12
GTTTTTT	60565	0.0	39.884422	2
CGTTTGG	1010	0.0	37.87129	2
CGAATAT	125	0.0	37.800003	14
GCTACGA	135	0.0	36.666668	10
TGGGCGA	3285	0.0	36.57534	6
TTGGGAC	4260	0.0	36.23239	5
TACGGGA	400	0.0	36.0	4
CGACCAA	905	0.0	35.552486	29
TCAAGCG	965	0.0	35.440414	17
TCGTTTG	540	0.0	35.416668	1
GGGCGAT	5315	0.0	35.390404	7
ACTTGCG	115	0.0	35.217392	1
CACGACC	915	0.0	35.163937	27
GACACGA	910	0.0	35.10989	25
CGAGACA	950	0.0	35.05263	22
TTGGGAT	6795	0.0	34.60265	5
ACACGAC	925	0.0	34.54054	26
>>END_MODULE