FastQCFastQC Report
Sat 14 Jan 2017
SRR2933741.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933741.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2005779
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT958444.778392833906428No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCC33260.16582086062322918No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA21480.10709056182161644No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGC20340.10140698451823456No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT533150.044.2108231
GTTTTTT609200.039.5412032
TCGACGG403.459936E-739.3752
TTCGACG356.250617E-638.571431
TGGGCGA34950.037.0815476
TTGGGAC44800.036.7131735
CGAATAT800.036.562514
TACGGGA3400.036.397064
CGTAGCG250.00210748236.01
TTTAGCG1200.035.6251
CGTTTGG10200.035.5147062
GGGCGAT55700.035.34567
TTGGGAT70800.035.30725
GCGATGC4800.035.156259
CACGACC8200.035.12195227
TTTGGGA121550.035.115184
CGACGGG900.035.03
GCGATGT6950.034.9640279
TTACACG15350.034.73941434
ACACGAC8300.034.69879526
Produced by FastQC (version 0.11.5)