##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933741.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2005779
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.47525076292054	34.0	31.0	34.0	31.0	34.0
2	32.70979006161696	34.0	31.0	34.0	31.0	34.0
3	32.76173895528869	34.0	31.0	34.0	31.0	34.0
4	36.198961101896074	37.0	37.0	37.0	35.0	37.0
5	36.25334695397648	37.0	37.0	37.0	35.0	37.0
6	36.246257937689045	37.0	37.0	37.0	35.0	37.0
7	36.26292328317327	37.0	37.0	37.0	35.0	37.0
8	36.2173115781948	37.0	37.0	37.0	35.0	37.0
9	38.075229125442036	39.0	39.0	39.0	37.0	39.0
10	37.609121942148164	39.0	38.0	39.0	35.0	39.0
11	37.496562183570575	39.0	37.0	39.0	35.0	39.0
12	37.49342973478135	39.0	37.0	39.0	35.0	39.0
13	37.52557086299139	39.0	37.0	39.0	35.0	39.0
14	38.89127715466161	40.0	38.0	41.0	35.0	41.0
15	38.93413382032617	40.0	38.0	41.0	35.0	41.0
16	39.005259801802694	40.0	38.0	41.0	35.0	41.0
17	38.964738886986055	40.0	38.0	41.0	35.0	41.0
18	38.51851874010048	39.0	38.0	41.0	35.0	41.0
19	38.0609100005534	39.0	37.0	41.0	35.0	41.0
20	37.47303765768811	39.0	35.0	41.0	34.0	41.0
21	37.404561519489434	39.0	35.0	41.0	34.0	41.0
22	37.376171053740215	39.0	35.0	41.0	34.0	41.0
23	37.23932646617598	38.0	35.0	41.0	34.0	41.0
24	37.15474785606989	38.0	35.0	41.0	34.0	41.0
25	37.10183823841012	38.0	35.0	41.0	33.0	41.0
26	37.08275687401254	38.0	35.0	41.0	34.0	41.0
27	37.00883098287498	38.0	35.0	41.0	33.0	41.0
28	36.91524589698067	38.0	35.0	41.0	33.0	41.0
29	36.73247800480512	38.0	35.0	41.0	33.0	41.0
30	36.42665966689251	38.0	35.0	40.0	33.0	41.0
31	35.99245430329064	38.0	35.0	41.0	31.0	41.0
32	35.33564914180476	38.0	35.0	41.0	25.0	41.0
33	34.39882210353184	38.0	35.0	41.0	18.0	41.0
34	33.60196960881533	38.0	33.0	41.0	15.0	41.0
35	33.09665571331637	38.0	33.0	41.0	10.0	41.0
36	32.769062793059454	38.0	33.0	41.0	9.0	41.0
37	32.597823090180924	38.0	32.0	41.0	8.0	41.0
38	32.41666055931386	38.0	31.0	40.0	8.0	41.0
39	32.338766135252186	38.0	31.0	40.0	8.0	41.0
40	32.178050024454336	38.0	31.0	40.0	8.0	41.0
41	32.01706120165781	37.0	30.0	40.0	7.0	41.0
42	31.8099486533661	37.0	30.0	40.0	7.0	41.0
43	31.699009212879385	37.0	29.0	40.0	7.0	41.0
44	31.506746755250703	37.0	28.0	40.0	7.0	41.0
45	31.474107566187502	37.0	27.0	40.0	7.0	41.0
46	31.354395474277077	36.0	27.0	40.0	7.0	41.0
47	31.32946949788586	36.0	27.0	40.0	7.0	41.0
48	31.210452397796566	36.0	26.0	40.0	7.0	41.0
49	31.178732552290157	36.0	27.0	40.0	7.0	41.0
50	31.030025242063058	36.0	26.0	40.0	7.0	41.0
51	30.32447891816596	35.0	24.0	40.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	5.0
9	7.0
10	4.0
11	10.0
12	17.0
13	26.0
14	36.0
15	66.0
16	126.0
17	292.0
18	642.0
19	1406.0
20	2434.0
21	4119.0
22	6604.0
23	11484.0
24	23356.0
25	49160.0
26	83217.0
27	93394.0
28	80688.0
29	64589.0
30	54383.0
31	50695.0
32	51499.0
33	60441.0
34	89473.0
35	115821.0
36	147239.0
37	181319.0
38	339711.0
39	493237.0
40	277.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.15649730104862	3.376742901386444	39.54942194528909	37.91733785227585
2	42.49331556467587	3.3682673913726284	40.04957674798669	14.088840295964811
3	16.29491584067836	3.670095259746961	65.67558041040414	14.35940848917054
4	13.596163884455864	3.7598359540108857	65.60996999170897	17.034030169824295
5	13.405415053203768	4.555237640836802	64.92569719794653	17.113650108012894
6	16.268641759635532	5.294651105630281	66.02182992243911	12.414877212295075
7	63.755179409097416	1.4570897391985855	32.066593577856786	2.7211372738472184
8	65.88422752456776	1.7102083529641103	30.334498466680525	2.071065655787602
9	61.25001807277871	4.208788705036796	31.53303529451649	3.0081579276680035
10	25.522153736777582	25.901457737866433	37.39868649537163	11.17770202998436
11	19.74973314607442	19.62270020774971	46.54156813886276	14.08599850731312
12	16.55730765951782	19.535950869961248	48.65281768330409	15.253923787216836
13	17.159068870498693	18.398387858283492	50.40749753587011	14.035045735347712
14	16.387747603300266	19.443468098928147	49.63278606466615	14.53599823310544
15	15.490191092837247	19.254813217208874	50.14640197150334	15.108593718450535
16	17.405407076253166	19.500752575433285	48.779252350333714	14.314587997979839
17	17.51120138360208	19.251024165673286	48.51446744631387	14.723307004410755
18	18.175631512743927	18.02297262061274	48.6196634823677	15.181732384275637
19	17.673083624865953	19.856075868777168	47.24463662247935	15.226203883877536
20	19.21283451466986	20.610146980300424	46.25529532416084	13.92172318086888
21	19.069398971671355	20.968511486061026	46.397334900804125	13.564754641463491
22	18.694731573119473	18.88039509836328	46.92311565730821	15.50175767120904
23	16.233393609166313	21.354795318925962	46.75225934661795	15.659551725289775
24	15.605458029025133	21.07390694587988	47.73138017697862	15.589254848116369
25	16.400211588614695	22.172831603082894	46.05183322788802	15.375123580414392
26	16.511190913854417	22.013641582646944	45.87688873001462	15.598278773484017
27	16.17670740395627	21.159858588608216	46.622135339935255	16.041298667500257
28	15.448860517534586	21.600186261796537	46.80879598400422	16.14215723666466
29	16.68723224243548	20.901405389128115	45.999085642037336	16.41227672639907
30	17.63030722726681	20.765049389788206	46.034283936565295	15.570359446379687
31	18.13699315826918	21.674870461800626	44.36336206531228	15.824774314617912
32	17.885120943035098	22.221889849280505	43.75482044632036	16.13816876136404
33	18.753262448156054	23.135250693122224	41.051581455384664	17.05990540333706
34	18.25216038257455	24.772569659967523	40.28823713878747	16.687032818670453
35	19.07917073615787	25.924042479256187	38.242249021452515	16.754537763133428
36	19.883047932997602	26.898227571432347	36.668695803475856	16.550028692094195
37	20.510784089373754	26.246460851369967	36.36567139251134	16.87708366674494
38	20.0135209312691	26.88207424646484	36.65518484339501	16.449219978871053
39	20.474937667609442	26.40973905898905	35.42100101755976	17.694322255841747
40	21.157664927192876	26.422701603716064	35.542549802346116	16.87708366674494
41	19.724007480385428	26.58268931921214	35.61872968058794	18.074573519814496
42	20.56941467629285	26.555168839637865	35.4157661437277	17.459650340341586
43	20.014867041683058	26.224723660981592	35.74626117832523	18.01414811901012
44	20.00858519308458	26.67377612389002	35.20033862155302	18.117300061472374
45	20.129386138752075	26.00510823974127	34.81719571298733	19.048309908519336
46	19.75561614714283	26.49384603189085	34.9404894557177	18.810048365248612
47	18.95188851812687	27.03533140989112	35.891690958974046	18.12108911300796
48	19.43544129238565	26.517527603988277	35.78026293026301	18.26676817336307
49	20.167575789755503	25.712055017028295	35.6197267994131	18.50064239380311
50	19.070396090496512	26.18743141692081	35.62506138512767	19.11711110745501
51	18.830938004635605	25.318193081092183	35.41716211008292	20.433706804189296
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	990.0
1	2701.5
2	4413.0
3	80324.5
4	156236.0
5	103352.5
6	50469.0
7	48562.5
8	46656.0
9	46335.0
10	46014.0
11	45270.0
12	44526.0
13	43177.5
14	41829.0
15	39549.5
16	37270.0
17	34952.0
18	32634.0
19	30529.5
20	28425.0
21	26973.0
22	25521.0
23	25071.5
24	24622.0
25	25004.5
26	26497.5
27	27608.0
28	29446.5
29	31285.0
30	34828.5
31	38372.0
32	42380.5
33	46389.0
34	51466.0
35	56543.0
36	61061.0
37	65579.0
38	69745.0
39	73911.0
40	78124.5
41	82338.0
42	87596.5
43	92855.0
44	98892.0
45	104929.0
46	112469.5
47	120010.0
48	123816.0
49	127622.0
50	125358.0
51	123094.0
52	114499.5
53	105905.0
54	95775.0
55	85645.0
56	77192.0
57	68739.0
58	60972.5
59	53206.0
60	47333.5
61	41461.0
62	36670.0
63	31879.0
64	26906.0
65	21933.0
66	17952.5
67	13972.0
68	11437.5
69	8903.0
70	7305.0
71	5707.0
72	5088.5
73	4470.0
74	4143.0
75	3176.5
76	2537.0
77	1926.5
78	1316.0
79	883.0
80	450.0
81	333.0
82	216.0
83	135.0
84	54.0
85	38.0
86	22.0
87	18.5
88	15.0
89	8.5
90	2.0
91	2.5
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2005779.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.65955963865938
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.60826636259684	28.097144566192
2	7.9522798948455815	5.989587179264983
3	3.7626637909052802	4.251007843014648
4	2.3768665628096297	3.580469923010584
5	1.7649302773337674	3.3233248518663325
6	1.3523217312253282	3.0556704532641206
7	1.1152282312241353	2.9399302859151355
8	0.9744606248900115	2.9358206414856545
9	0.8220950993406686	2.786376548004257
>10	5.1504648007264136	31.364635948031715
>50	0.06830360853845718	1.790227897969366
>100	0.04730856933727694	3.2966380334144874
>500	0.0030733406448729917	0.793829929323663
>1k	0.0016034820755859084	0.9722718265903324
>5k	0.0	0.0
>10k+	1.3362350629882573E-4	4.823064072652752
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	95844	4.778392833906428	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCC	3326	0.16582086062322918	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	2148	0.10709056182161644	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGC	2034	0.10140698451823456	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.994237650309431E-4	0.0	0.0	0.05957784980299425	0.0
2	1.994237650309431E-4	0.0	0.0	0.17165400575038425	0.0
3	2.4927970628867884E-4	0.0	0.0	0.2853255518180218	0.0
4	2.991356475464146E-4	0.0	0.0	0.42766426410885744	0.0
5	2.991356475464146E-4	0.0	0.0	0.7563146288798517	0.0
6	2.991356475464146E-4	0.0	0.0	1.3308544959339987	0.0
7	2.991356475464146E-4	0.0	0.0	1.8541424553751933	0.0
8	2.991356475464146E-4	0.0	0.0	2.5911628349883014	0.0
9	2.991356475464146E-4	0.0	0.0	3.135789137287807	0.0
10	2.991356475464146E-4	0.0	0.0	3.8092930477385596	0.0
11	2.991356475464146E-4	0.0	0.0	4.634458731495344	0.0
12	3.489915888041504E-4	0.0	0.0	5.300484250757436	0.0
13	3.489915888041504E-4	0.0	0.0	5.619213283218142	0.0
14	3.489915888041504E-4	0.0	0.0	5.786479966137844	0.0
15	3.489915888041504E-4	0.0	0.0	5.935100527027155	0.0
16	3.489915888041504E-4	0.0	0.0	6.146788853607501	0.0
17	3.489915888041504E-4	0.0	0.0	6.4154625210454395	0.0
18	3.489915888041504E-4	0.0	0.0	6.6968494535041	0.0
19	3.988475300618862E-4	0.0	0.0	6.889093962993929	0.0
20	3.988475300618862E-4	0.0	0.0	7.075106479826541	0.0
21	3.988475300618862E-4	0.0	0.0	7.275477507741381	0.0
22	3.988475300618862E-4	0.0	0.0	7.488462088794429	0.0
23	3.988475300618862E-4	0.0	0.0	7.6822022765219895	0.0
24	3.988475300618862E-4	0.0	0.0	7.844333797492147	0.0
25	3.988475300618862E-4	0.0	0.0	7.987520060784363	0.0
26	4.985594125773577E-4	0.0	0.0	8.126618136893446	0.0
27	4.985594125773577E-4	0.0	0.0	8.276036392842881	0.0
28	4.985594125773577E-4	0.0	0.0	8.43457828604248	0.0
29	4.985594125773577E-4	0.0	0.0	8.61186601315499	0.0
30	4.985594125773577E-4	0.0	0.0	8.823604195676593	0.0
31	4.985594125773577E-4	4.985594125773577E-5	0.0	8.984638885939079	0.0
32	4.985594125773577E-4	4.985594125773577E-5	0.0	9.174839301837341	0.0
33	4.985594125773577E-4	4.985594125773577E-5	0.0	9.339214340164096	0.0
34	4.985594125773577E-4	4.985594125773577E-5	0.0	9.504636353257263	0.0
35	4.985594125773577E-4	4.985594125773577E-5	0.0	9.698177117219794	0.0
36	4.985594125773577E-4	4.985594125773577E-5	0.0	9.8596605109536	0.0
37	4.985594125773577E-4	4.985594125773577E-5	0.0	10.029320279053675	0.0
38	5.484153538350935E-4	4.985594125773577E-5	0.0	10.236222435273278	0.0
39	5.484153538350935E-4	4.985594125773577E-5	0.0	10.519454037558475	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	53315	0.0	44.210823	1
GTTTTTT	60920	0.0	39.541203	2
TCGACGG	40	3.459936E-7	39.375	2
TTCGACG	35	6.250617E-6	38.57143	1
TGGGCGA	3495	0.0	37.081547	6
TTGGGAC	4480	0.0	36.713173	5
CGAATAT	80	0.0	36.5625	14
TACGGGA	340	0.0	36.39706	4
CGTAGCG	25	0.002107482	36.0	1
TTTAGCG	120	0.0	35.625	1
CGTTTGG	1020	0.0	35.514706	2
GGGCGAT	5570	0.0	35.3456	7
TTGGGAT	7080	0.0	35.3072	5
GCGATGC	480	0.0	35.15625	9
CACGACC	820	0.0	35.121952	27
TTTGGGA	12155	0.0	35.11518	4
CGACGGG	90	0.0	35.0	3
GCGATGT	695	0.0	34.964027	9
TTACACG	1535	0.0	34.739414	34
ACACGAC	830	0.0	34.698795	26
>>END_MODULE