FastQCFastQC Report
Sat 14 Jan 2017
SRR2933740.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933740.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1642477
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT752974.5843564323883985No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCC38150.23227113682566025No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGC21570.13132603987757516No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA16830.10246718827721789No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT433050.044.3037721
GTTTTTT493850.039.6329842
TGGGCGA27100.038.856096
GCCGATG2050.038.4146359
GCCGATA301.1401354E-437.4999969
TTGGGCG20700.036.956525
TTGGGAC34450.036.8359955
AACACGT5400.036.66666441
TTTACGG2150.036.6279072
GGGCGAT41950.036.5792587
CTTTGCG5050.036.5346571
GGGCCGA6850.036.4598547
GGCCGAT6800.036.0661778
CTCGTAG250.002107371336.01
TCGATCA250.002107371336.017
GCTACGA1000.036.010
CACGACC6750.035.66666827
CGTGCGG3550.035.4929582
ACACGAC6850.035.47445326
ACACGTG5650.035.4424842
Produced by FastQC (version 0.11.5)