##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933740.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1642477
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.570614991868986	34.0	31.0	34.0	31.0	34.0
2	32.766360807487715	34.0	31.0	34.0	31.0	34.0
3	32.80501949190156	34.0	31.0	34.0	31.0	34.0
4	36.26277506473454	37.0	37.0	37.0	35.0	37.0
5	36.27069541917482	37.0	37.0	37.0	35.0	37.0
6	36.25041994499771	37.0	37.0	37.0	35.0	37.0
7	36.321150920225975	37.0	37.0	37.0	35.0	37.0
8	36.24435471546938	37.0	37.0	37.0	35.0	37.0
9	38.08590500810666	39.0	39.0	39.0	37.0	39.0
10	37.615970269294486	39.0	38.0	39.0	35.0	39.0
11	37.48436599112195	39.0	37.0	39.0	35.0	39.0
12	37.51923162394359	39.0	37.0	39.0	35.0	39.0
13	37.534517073907274	39.0	37.0	39.0	35.0	39.0
14	38.8843478477933	40.0	38.0	41.0	35.0	41.0
15	38.927065036527146	40.0	38.0	41.0	35.0	41.0
16	38.93388887637391	40.0	38.0	41.0	35.0	41.0
17	38.86065984485627	40.0	38.0	41.0	35.0	41.0
18	38.405784677654545	39.0	38.0	41.0	35.0	41.0
19	37.93839609321774	39.0	37.0	41.0	35.0	41.0
20	37.415623476006054	39.0	35.0	41.0	34.0	41.0
21	37.30970905528662	38.0	35.0	41.0	34.0	41.0
22	37.3024340675699	38.0	35.0	41.0	34.0	41.0
23	37.199102331417734	38.0	35.0	41.0	34.0	41.0
24	37.124644058942685	38.0	35.0	41.0	33.0	41.0
25	37.06474854746824	38.0	35.0	40.0	33.0	41.0
26	37.01974030686579	38.0	35.0	40.0	33.0	41.0
27	36.958262429245586	38.0	35.0	40.0	33.0	41.0
28	36.8087480068214	38.0	35.0	40.0	33.0	41.0
29	36.66844345461154	38.0	35.0	40.0	33.0	41.0
30	36.4509834840914	38.0	35.0	40.0	33.0	41.0
31	36.03204550200703	38.0	35.0	40.0	31.0	41.0
32	35.34650591758667	38.0	35.0	40.0	25.0	41.0
33	34.49151251433049	38.0	35.0	41.0	18.0	41.0
34	33.73727607753411	38.0	33.0	41.0	15.0	41.0
35	33.22231057116782	38.0	33.0	41.0	10.0	41.0
36	32.95630014910407	38.0	33.0	41.0	10.0	41.0
37	32.7622481167164	38.0	33.0	41.0	9.0	41.0
38	32.602938123334454	38.0	32.0	40.0	8.0	41.0
39	32.47933578369743	38.0	31.0	40.0	8.0	41.0
40	32.336305470335354	38.0	31.0	40.0	8.0	41.0
41	32.18117574857974	38.0	31.0	40.0	8.0	41.0
42	32.027851227140474	37.0	30.0	40.0	8.0	41.0
43	31.872556510684777	37.0	30.0	40.0	7.0	41.0
44	31.716750371542492	37.0	29.0	40.0	7.0	41.0
45	31.694904099113717	37.0	29.0	40.0	7.0	41.0
46	31.565777176788473	37.0	28.0	40.0	7.0	41.0
47	31.468340195935774	37.0	28.0	40.0	7.0	41.0
48	31.383215716262693	36.0	27.0	40.0	7.0	41.0
49	31.3407091849688	36.0	27.0	40.0	7.0	41.0
50	31.251218129690706	36.0	27.0	40.0	7.0	41.0
51	30.413398787319395	35.0	24.0	40.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	4.0
10	11.0
11	2.0
12	10.0
13	18.0
14	20.0
15	36.0
16	82.0
17	219.0
18	509.0
19	1107.0
20	1919.0
21	3278.0
22	5302.0
23	9511.0
24	18661.0
25	39236.0
26	65203.0
27	74429.0
28	64660.0
29	51806.0
30	44005.0
31	41063.0
32	42541.0
33	49728.0
34	74817.0
35	95569.0
36	121291.0
37	153274.0
38	289857.0
39	394195.0
40	112.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.524034126505274	3.4075971840092736	39.80177500202438	38.26659368746107
2	41.37646980749198	3.5319216037728385	40.83113492609029	14.260473662644896
3	16.569668859898798	3.907086674577483	65.89821349096518	13.625030974558547
4	13.812978811879862	3.799383492128048	65.23141572149869	17.156221974493402
5	13.59032729225432	4.505938287111479	64.4273861977976	17.476348222836606
6	16.416181170269052	5.18235567377808	65.28791575163609	13.113547404316773
7	63.806312051858264	1.543644142353287	31.603182266783648	3.0468615390048077
8	65.94351092892016	1.6727174870637458	29.905746016534785	2.47802556748131
9	61.175590282238346	4.255158519723564	31.375172985679555	3.1940782123585296
10	25.450158510591013	27.702853677707513	36.23033990734726	10.616647904354217
11	19.2576212634941	19.45798936606114	47.23554728620248	14.048842084242274
12	16.864284857565735	19.50121675980851	48.4681368445342	15.166361538091552
13	16.821057463818367	18.227469851937045	50.38932052016558	14.56215216407901
14	16.013253153621022	19.499451133866717	49.648792646715904	14.838503065796354
15	15.27662183397393	19.11204844877584	50.14986511226641	15.461464604983814
16	17.31421505445738	19.939091993373424	48.47982650594194	14.266866446227253
17	16.865502530629044	19.236190217579914	48.36895737352791	15.529349878263135
18	17.81114743159265	17.941925518591738	48.63355773018435	15.613369319631264
19	17.27713690967971	19.97251711896118	47.346964371495005	15.403381599864108
20	18.956125413019482	20.85289474373157	46.00082680000999	14.190153043238961
21	18.6844016689427	21.448884824566797	46.06578965793737	13.800923848553131
22	18.342052887194157	19.1259908053507	46.58908465689321	15.942871650561926
23	15.908655037483022	21.833547745265232	46.339705213528106	15.918092003723643
24	15.354126724453371	21.297406295491506	47.34057158791264	16.007895392142476
25	15.95462219562283	22.47313052176682	45.62158252444327	15.950664758167084
26	15.974592033861052	22.42637187613586	45.76155404307031	15.837482046932772
27	16.030483227466807	21.515613308435977	46.36734639206516	16.086557072032058
28	15.053300594163572	21.82404989537144	46.50804851453019	16.614600995934797
29	16.402482348306858	20.269020509876242	46.557607808206754	16.77088933361015
30	17.514765807983917	19.89044595449434	46.813684453420045	15.781103784101697
31	17.62715703172708	21.52651148235257	44.70071727031794	16.14561421560241
32	17.438600357874112	21.653514782855407	44.385766132493785	16.52211872677669
33	17.826124810271317	22.591244808907522	42.213559154861834	17.369071225959328
34	18.269540456274274	23.378957513560312	41.10200629902276	17.24949573114266
35	17.545816471098224	24.95231287865827	39.684208667762164	17.817661982481336
36	18.604339665030317	26.07628599974307	37.60089182375157	17.71848251147505
37	19.012138373931567	25.955127529944104	36.5398724000397	18.492861696084635
38	19.04885121679025	26.501984502674926	36.518624005085	17.930540275449825
39	19.472297024554987	25.864837072299945	35.62473020931191	19.038135693833155
40	20.18786260020688	25.725048204632394	35.229534416615884	18.857554778544845
41	18.517336924657087	26.37973012711898	34.93863232179202	20.16430062643191
42	19.215976844729028	26.90065066360138	35.168346345184744	18.71502614648485
43	18.951437371725753	26.203776369471232	36.167751511893314	18.677034746909698
44	19.53238919022915	26.29114441176345	35.519645023948584	18.656821374058815
45	19.750717970479954	25.197186931689146	35.42649303460566	19.62560206322524
46	19.592664006862805	25.40857497547911	35.40494022138514	19.593820796272947
47	18.3730426666553	26.48371940672533	36.1233673287358	19.01987059788356
48	18.533105790826905	25.87530906064438	36.53037455014591	19.061210598382807
49	19.532693608494974	25.017336620238822	36.25712871473999	19.192841056526213
50	18.546865496442262	25.09057965499669	36.14802520826776	20.21452964029329
51	18.023570497486418	24.473950015738424	35.866986265256685	21.635493221518477
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	831.0
1	2348.5
2	3866.0
3	65070.0
4	126274.0
5	83314.0
6	40354.0
7	38982.0
8	37610.0
9	37304.5
10	36999.0
11	36282.5
12	35566.0
13	34336.0
14	33106.0
15	31386.0
16	29666.0
17	27805.5
18	25945.0
19	24098.5
20	22252.0
21	21401.0
22	20550.0
23	19744.5
24	18939.0
25	19236.0
26	20996.5
27	22460.0
28	24006.0
29	25552.0
30	28524.5
31	31497.0
32	35121.0
33	38745.0
34	43413.5
35	48082.0
36	51684.5
37	55287.0
38	57234.0
39	59181.0
40	64065.5
41	68950.0
42	73479.5
43	78009.0
44	82379.5
45	86750.0
46	94372.0
47	101994.0
48	106877.5
49	111761.0
50	110703.0
51	109645.0
52	98896.5
53	88148.0
54	78715.5
55	69283.0
56	61063.0
57	52843.0
58	47230.0
59	41617.0
60	36476.0
61	31335.0
62	27241.0
63	23147.0
64	19186.0
65	15225.0
66	12646.5
67	10068.0
68	8278.0
69	6488.0
70	5348.0
71	4208.0
72	3777.0
73	3346.0
74	3236.5
75	2702.5
76	2278.0
77	1702.5
78	1127.0
79	831.5
80	536.0
81	371.0
82	206.0
83	127.5
84	49.0
85	34.0
86	19.0
87	14.0
88	9.0
89	9.5
90	10.0
91	7.0
92	4.0
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1642477.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.99750108967739
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.93491874003848	27.724047377394605
2	7.82879786993593	5.7929191544763725
3	3.576892894697529	3.9700829630769348
4	2.285090829862669	3.3817060187142367
5	1.6460519393962654	3.044990421073946
6	1.3385020365878955	2.971273833431764
7	1.1002324282117857	2.849409532316476
8	0.9423866163890248	2.7892759893400267
9	0.8130806854854635	2.7073758192519666
>10	5.387645864790147	32.53841182932706
>50	0.09091733008462606	2.313605663069311
>100	0.05036386910380302	3.484974780469953
>500	0.0034676388304818884	0.912719968026599
>1k	0.0014861309273493809	0.919134666597067
>5k	0.0	0.0
>10k+	1.6512565859437564E-4	4.600071983433656
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	75297	4.5843564323883985	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCC	3815	0.23227113682566025	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGC	2157	0.13132603987757516	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	1683	0.10246718827721789	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8265095949593206E-4	0.0	0.0	0.08578506730992276	0.0
2	1.8265095949593206E-4	0.0	0.0	0.25035358181575756	0.0
3	1.8265095949593206E-4	0.0	0.0	0.4011015070530668	0.0
4	1.8265095949593206E-4	0.0	0.0	0.5866139982477685	0.0
5	1.8265095949593206E-4	0.0	0.0	1.0098771550530083	0.0
6	1.8265095949593206E-4	0.0	0.0	1.782125411801809	0.0
7	1.8265095949593206E-4	0.0	0.0	2.5047534912208818	0.0
8	1.8265095949593206E-4	0.0	0.0	3.486685049471012	0.0
9	1.8265095949593206E-4	0.0	0.0	4.14849035937794	0.0
10	1.8265095949593206E-4	0.0	0.0	4.988684773059227	0.0
11	1.8265095949593206E-4	0.0	0.0	6.15832063401801	0.0
12	1.8265095949593206E-4	0.0	0.0	7.099155726381556	0.0
13	1.8265095949593206E-4	0.0	0.0	7.503362299746054	0.0
14	1.8265095949593206E-4	0.0	0.0	7.705496028254886	0.0
15	1.8265095949593206E-4	0.0	0.0	7.891008519449588	0.0
16	1.8265095949593206E-4	0.0	0.0	8.198227433321746	0.0
17	1.8265095949593206E-4	0.0	0.0	8.617594036324405	0.0
18	2.4353461266124273E-4	0.0	0.0	9.040613658516984	0.0
19	2.4353461266124273E-4	0.0	0.0	9.32110464864957	0.0
20	3.044182658265534E-4	0.0	0.0	9.603726566642942	0.0
21	3.044182658265534E-4	0.0	0.0	9.903578558482097	0.0
22	3.044182658265534E-4	0.0	0.0	10.20166492437946	0.0
23	3.044182658265534E-4	0.0	0.0	10.461516356089005	0.0
24	3.653019189918641E-4	0.0	0.0	10.660301483673743	0.0
25	3.653019189918641E-4	0.0	0.0	10.836377008627823	0.0
26	3.653019189918641E-4	0.0	0.0	11.001797894277972	0.0
27	3.653019189918641E-4	0.0	0.0	11.177386350006728	0.0
28	3.653019189918641E-4	0.0	0.0	11.357784614335543	0.0
29	3.653019189918641E-4	0.0	0.0	11.545915102616354	0.0
30	4.261855721571748E-4	0.0	0.0	11.781108654793949	0.0
31	4.8706922532248546E-4	0.0	0.0	11.96053278067212	0.0
32	4.8706922532248546E-4	0.0	0.0	12.171981708115243	0.0
33	9.132547974796603E-4	0.0	0.0	12.352197321484562	0.0
34	9.132547974796603E-4	0.0	0.0	12.524558943595558	0.0
35	9.132547974796603E-4	0.0	0.0	12.732476619155094	0.0
36	9.132547974796603E-4	0.0	0.0	12.916345251714331	0.0
37	9.132547974796603E-4	0.0	0.0	13.10605871497744	0.0
38	9.132547974796603E-4	0.0	0.0	13.335529203757496	0.0
39	9.132547974796603E-4	0.0	0.0	13.609140341082401	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	43305	0.0	44.303772	1
GTTTTTT	49385	0.0	39.632984	2
TGGGCGA	2710	0.0	38.85609	6
GCCGATG	205	0.0	38.414635	9
GCCGATA	30	1.1401354E-4	37.499996	9
TTGGGCG	2070	0.0	36.95652	5
TTGGGAC	3445	0.0	36.835995	5
AACACGT	540	0.0	36.666664	41
TTTACGG	215	0.0	36.627907	2
GGGCGAT	4195	0.0	36.579258	7
CTTTGCG	505	0.0	36.534657	1
GGGCCGA	685	0.0	36.459854	7
GGCCGAT	680	0.0	36.066177	8
CTCGTAG	25	0.0021073713	36.0	1
TCGATCA	25	0.0021073713	36.0	17
GCTACGA	100	0.0	36.0	10
CACGACC	675	0.0	35.666668	27
CGTGCGG	355	0.0	35.492958	2
ACACGAC	685	0.0	35.474453	26
ACACGTG	565	0.0	35.44248	42
>>END_MODULE