FastQCFastQC Report
Sat 14 Jan 2017
SRR2933739.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933739.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1647499
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT740874.496937479172977No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCC36470.22136583997926554No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGC21160.12843710375544992No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA16760.1017299555265284No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT421900.044.328041
GTTTTTT489150.039.1168372
TACGCGG750.039.02
TTATCCG356.2499385E-638.5714261
CGCACGG356.2499385E-638.5714262
TGGGCGA28100.038.5142366
GGGACCG9600.038.203127
TTGGGAC34500.037.0434765
GCGAGAC7550.036.95364421
TTGGGCG22500.036.65
GTTTGCG2400.036.56251
TACGGGA2900.036.4655154
GACACGA7250.036.31034525
ACGACCA7250.036.31034528
GGGCGAT43350.036.2802777
TCAAGCG7700.036.23376517
GACCGAT8100.036.111119
TTGGGAT64500.035.930235
CGACCAA7300.035.75342629
ACACGAC7500.035.726
Produced by FastQC (version 0.11.5)