##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933739.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1647499
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.50325250576783	34.0	31.0	34.0	31.0	34.0
2	32.71863897944703	34.0	31.0	34.0	31.0	34.0
3	32.76499651896602	34.0	31.0	34.0	31.0	34.0
4	36.194913623619804	37.0	37.0	37.0	35.0	37.0
5	36.25241411375667	37.0	37.0	37.0	35.0	37.0
6	36.24958254906376	37.0	37.0	37.0	35.0	37.0
7	36.260626561837064	37.0	37.0	37.0	35.0	37.0
8	36.21384534983026	37.0	37.0	37.0	35.0	37.0
9	38.065721435946244	39.0	39.0	39.0	37.0	39.0
10	37.642079297164976	39.0	38.0	39.0	35.0	39.0
11	37.499037025212154	39.0	37.0	39.0	35.0	39.0
12	37.51048407313146	39.0	37.0	39.0	35.0	39.0
13	37.53435419384169	39.0	37.0	39.0	35.0	39.0
14	38.91525882565028	40.0	38.0	41.0	35.0	41.0
15	38.94611772146751	40.0	38.0	41.0	35.0	41.0
16	39.00684370673366	40.0	38.0	41.0	35.0	41.0
17	38.95076962110448	40.0	38.0	41.0	35.0	41.0
18	38.53470927751701	39.0	38.0	41.0	35.0	41.0
19	38.097949680090856	39.0	37.0	41.0	35.0	41.0
20	37.534921113760916	39.0	35.0	41.0	34.0	41.0
21	37.46460726228059	39.0	35.0	41.0	34.0	41.0
22	37.44082576074401	39.0	35.0	41.0	34.0	41.0
23	37.29086208853541	38.0	35.0	41.0	34.0	41.0
24	37.2198374627238	38.0	35.0	41.0	34.0	41.0
25	37.17398371713731	38.0	35.0	41.0	34.0	41.0
26	37.147954566285016	38.0	35.0	41.0	34.0	41.0
27	37.090909311629325	38.0	35.0	41.0	33.0	41.0
28	36.9895896750165	38.0	35.0	41.0	33.0	41.0
29	36.80309001705009	38.0	35.0	41.0	33.0	41.0
30	36.504189076897774	38.0	35.0	41.0	33.0	41.0
31	36.08331294890012	38.0	35.0	41.0	31.0	41.0
32	35.474482230338225	38.0	35.0	41.0	25.0	41.0
33	34.5745788009583	38.0	35.0	41.0	18.0	41.0
34	33.83237319112182	38.0	34.0	41.0	15.0	41.0
35	33.37105819184109	38.0	33.0	41.0	10.0	41.0
36	33.0936279779229	38.0	33.0	41.0	10.0	41.0
37	32.92668341528584	38.0	33.0	41.0	10.0	41.0
38	32.754578303234176	38.0	33.0	41.0	9.0	41.0
39	32.668252302429316	38.0	32.0	41.0	8.0	41.0
40	32.516269812606865	38.0	31.0	41.0	8.0	41.0
41	32.37784544937508	38.0	31.0	41.0	8.0	41.0
42	32.173198891167765	38.0	31.0	40.0	8.0	41.0
43	32.05338819629026	38.0	30.0	40.0	8.0	41.0
44	31.89744758570415	37.0	30.0	40.0	7.0	41.0
45	31.84571219770088	37.0	30.0	40.0	7.0	41.0
46	31.709600430713465	37.0	29.0	40.0	7.0	41.0
47	31.66434334709763	37.0	29.0	40.0	7.0	41.0
48	31.543530527180895	37.0	28.0	40.0	7.0	41.0
49	31.50436752920639	36.0	28.0	40.0	7.0	41.0
50	31.36052707770991	36.0	27.0	40.0	7.0	41.0
51	30.636437412101614	35.0	25.0	40.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	5.0
10	9.0
11	15.0
12	11.0
13	27.0
14	27.0
15	45.0
16	89.0
17	235.0
18	555.0
19	966.0
20	1884.0
21	3111.0
22	5228.0
23	8929.0
24	18293.0
25	38232.0
26	64014.0
27	74113.0
28	64210.0
29	51344.0
30	43375.0
31	40665.0
32	41465.0
33	48276.0
34	73333.0
35	94253.0
36	118483.0
37	147844.0
38	283397.0
39	424855.0
40	210.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.688691161572784	3.543006702887225	39.69477371458192	38.07352842095807
2	41.27632247424733	3.5919293425974765	40.84038897747434	14.291359205680854
3	16.81227120623442	3.975662504195754	65.56562401555327	13.646442274016554
4	14.004075268027478	3.879395374443323	64.94140512376639	17.175124233762812
5	13.6925728027756	4.547013382102205	64.2535139626792	17.506899852443006
6	16.516853727984053	5.208136696896326	65.10213359765316	13.17287597746645
7	63.93515261617762	1.5808810809596852	31.373615401284006	3.110350901578696
8	66.09163343953472	1.7040374531335074	29.72548086523876	2.4788482420930147
9	61.30437711950053	4.307134632555163	31.16766686960053	3.2208213783437807
10	25.57810353754388	27.808757395300393	35.990370859102185	10.62276820805354
11	19.365353180791008	19.603957270990758	46.98467191785852	14.046017630359714
12	16.98689953681307	19.601165160039553	48.27414159280218	15.137793710345196
13	16.872483685877807	18.309206864465473	50.24919590239508	14.569113547261637
14	16.08195209830173	19.68049752989228	49.38133498108345	14.856215390722543
15	15.255426558680766	19.276369818737372	49.98940818780467	15.478795434777199
16	17.44347037539932	19.99388163513301	48.288344939814834	14.274303049652836
17	16.939737140963363	19.290208977365083	48.2935649733323	15.476488908339247
18	17.978705905132568	18.020284079079865	48.42230556740854	15.578704448379026
19	17.361103102338756	20.083957562341464	47.17386778383477	15.38107155148501
20	19.083896257296665	20.90848006584526	45.83505058273176	14.172573094126308
21	18.760132783085147	21.556189108460764	45.872744080573035	13.81093402788105
22	18.52747710317275	19.088448612108415	46.426067633424964	15.958006651293871
23	16.02610987927762	21.91879934373253	46.19608266833546	15.85900810865439
24	15.444925914977794	21.302349804157696	47.208344284275746	16.04437999658877
25	15.981193311801706	22.59855696422274	45.490649766706994	15.929599957268561
26	16.087172131819198	22.486447639725426	45.58418548357237	15.842194744883
27	16.17154244099693	21.60626501138999	46.10776698498755	16.11442556262553
28	15.198613170630148	21.882926787815958	46.344671529390915	16.57378851216298
29	16.454334721902715	20.453912263376182	46.35086273193489	16.74089028278621
30	17.66975275857527	19.93391194774625	46.64834394436658	15.747991349311896
31	17.724198922123776	21.586173952154144	44.51954143826491	16.17008568745717
32	17.506414267929753	21.74356403251231	44.28196921515582	16.468052484402115
33	17.93585307183798	22.681895406309806	42.02375843627219	17.358493085580022
34	18.30344054837059	23.5342783212615	41.028249486039144	17.13403164432877
35	17.56680884176561	25.065447687676894	39.596139360327385	17.771604110230115
36	18.61925257617759	26.153399789620508	37.552860426622416	17.67448720757949
37	19.059738427762323	25.86144210102707	36.47789771040833	18.600921760802283
38	19.141013135668064	26.557284708518793	36.42442271588632	17.87727943992682
39	19.437280386816624	25.863323741015925	35.61076516586657	19.088630706300886
40	20.206628350002035	25.87018262226563	35.20257068441317	18.720618343319178
41	18.596551500183004	26.396616932696165	34.897805704282675	20.109025862838156
42	19.25955645496598	27.016222771607147	35.088215531542055	18.63600524188482
43	18.935064603984586	26.32305087893832	36.087669855945286	18.654214661131814
44	19.574093823425688	26.404750473293152	35.43504427013309	18.586111433148062
45	19.65828203841095	25.45701089955138	35.260476637618595	19.62423042441907
46	19.62714393149859	25.54775450546556	35.287972860681556	19.537128702354295
47	18.410511933542903	26.635099626767605	36.10266227779198	18.85172616189752
48	18.59606591566975	26.013308657546986	36.396562304438426	18.99406312234484
49	19.513638551525673	25.2012292571953	36.19401286434772	19.091119326931306
50	18.56778061777276	25.272852972900132	36.015499857663045	20.14386655166407
51	18.117825868179587	24.627086268337646	35.71619770330665	21.538890160176123
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	792.0
1	2328.0
2	3864.0
3	64385.5
4	124907.0
5	82862.0
6	40817.0
7	39314.0
8	37811.0
9	37581.5
10	37352.0
11	36432.5
12	35513.0
13	34110.0
14	32707.0
15	31194.0
16	29681.0
17	27790.5
18	25900.0
19	24060.0
20	22220.0
21	21241.5
22	20263.0
23	19525.0
24	18787.0
25	19210.5
26	20862.0
27	22090.0
28	23909.5
29	25729.0
30	29003.0
31	32277.0
32	35512.5
33	38748.0
34	43355.0
35	47962.0
36	52066.0
37	56170.0
38	57922.0
39	59674.0
40	64797.0
41	69920.0
42	74367.5
43	78815.0
44	82988.5
45	87162.0
46	94596.5
47	102031.0
48	106971.5
49	111912.0
50	110873.0
51	109834.0
52	99177.5
53	88521.0
54	78873.5
55	69226.0
56	61239.0
57	53252.0
58	47544.0
59	41836.0
60	36858.0
61	31880.0
62	27595.0
63	23310.0
64	19303.0
65	15296.0
66	12653.0
67	10010.0
68	8263.5
69	6517.0
70	5389.5
71	4262.0
72	3854.0
73	3446.0
74	3322.0
75	2717.0
76	2236.0
77	1689.0
78	1142.0
79	827.0
80	512.0
81	352.0
82	192.0
83	124.0
84	56.0
85	34.5
86	13.0
87	8.0
88	3.0
89	8.0
90	13.0
91	9.5
92	6.0
93	3.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1647499.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.97843916849783
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.72188173248767	27.63098558200484
2	7.930152425360385	5.864893181162089
3	3.5805022465674465	3.972041535520924
4	2.3222191102341236	3.4348815241486275
5	1.6857782248467468	3.116872376953684
6	1.3390002056359704	2.970848259042949
7	1.1238584536539702	2.909097202371176
8	0.9292914559416988	2.749099805867591
9	0.8089588342223971	2.6922631537000647
>10	5.410711201110254	32.578786696290265
>50	0.09201259807963286	2.3131266185464856
>100	0.050335133866238974	3.3907005851338043
>500	0.0036426348705550222	0.9343821391241804
>1k	0.0014901688106815998	0.905911678820613
>5k	0.0	0.0
>10k+	1.6557431229795552E-4	4.536109661312703
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	74087	4.496937479172977	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCC	3647	0.22136583997926554	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGC	2116	0.12843710375544992	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	1676	0.1017299555265284	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.641883849398391E-4	0.0	0.0	0.08248866918887356	0.0
2	3.641883849398391E-4	0.0	0.0	0.24515948112866837	0.0
3	3.641883849398391E-4	0.0	0.0	0.3902278544630376	0.0
4	3.641883849398391E-4	0.0	0.0	0.5684373708269322	0.0
5	3.641883849398391E-4	0.0	0.0	0.9810021128996133	0.0
6	3.641883849398391E-4	0.0	0.0	1.7203045343274868	0.0
7	3.641883849398391E-4	0.0	0.0	2.4353884281568607	0.0
8	3.641883849398391E-4	0.0	0.0	3.4013374211456275	0.0
9	4.8558451325311883E-4	0.0	0.0	4.046922031515649	0.0
10	5.462825774097587E-4	0.0	0.0	4.887043937507701	0.0
11	5.462825774097587E-4	0.0	0.0	6.023068906263372	0.0
12	5.462825774097587E-4	0.0	0.0	6.943494351134659	0.0
13	5.462825774097587E-4	0.0	0.0	7.327591701117877	0.0
14	5.462825774097587E-4	0.0	0.0	7.520611545135991	0.0
15	5.462825774097587E-4	0.0	0.0	7.702645039541754	0.0
16	6.069806415663986E-4	0.0	0.0	8.0056497758117	0.0
17	6.069806415663986E-4	0.0	0.0	8.40091556959974	0.0
18	6.069806415663986E-4	0.0	0.0	8.806742826551034	0.0
19	6.069806415663986E-4	0.0	0.0	9.073085932070368	0.0
20	6.069806415663986E-4	0.0	0.0	9.338093680178257	0.0
21	6.069806415663986E-4	0.0	0.0	9.6259845984732	0.0
22	6.069806415663986E-4	0.0	0.0	9.919034852221458	0.0
23	6.069806415663986E-4	0.0	0.0	10.185377957740794	0.0
24	6.069806415663986E-4	0.0	0.0	10.380643630132704	0.0
25	6.069806415663986E-4	0.0	0.0	10.551690774926115	0.0
26	6.069806415663986E-4	0.0	0.0	10.708655968835187	0.0
27	6.069806415663986E-4	0.0	0.0	10.876243323971668	0.0
28	6.069806415663986E-4	0.0	0.0	11.053299577116587	0.0
29	6.676787057230384E-4	0.0	0.0	11.236971919254579	0.0
30	6.676787057230384E-4	0.0	0.0	11.461736850826616	0.0
31	6.676787057230384E-4	0.0	0.0	11.642010101371836	0.0
32	7.283767698796782E-4	0.0	0.0	11.848383519504413	0.0
33	0.0013353574114460768	0.0	0.0	12.029810033268609	0.0
34	0.0013353574114460768	0.0	0.0	12.208140945760817	0.0
35	0.0013353574114460768	0.0	0.0	12.407958972964476	0.0
36	0.0013353574114460768	0.0	0.0	12.59539459508018	0.0
37	0.0013353574114460768	0.0	0.0	12.783558593965763	0.0
38	0.0013353574114460768	0.0	0.0	13.00632048942063	0.0
39	0.0013353574114460768	0.0	0.0	13.277822930393281	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	42190	0.0	44.32804	1
GTTTTTT	48915	0.0	39.116837	2
TACGCGG	75	0.0	39.0	2
TTATCCG	35	6.2499385E-6	38.571426	1
CGCACGG	35	6.2499385E-6	38.571426	2
TGGGCGA	2810	0.0	38.514236	6
GGGACCG	960	0.0	38.20312	7
TTGGGAC	3450	0.0	37.043476	5
GCGAGAC	755	0.0	36.953644	21
TTGGGCG	2250	0.0	36.6	5
GTTTGCG	240	0.0	36.5625	1
TACGGGA	290	0.0	36.465515	4
GACACGA	725	0.0	36.310345	25
ACGACCA	725	0.0	36.310345	28
GGGCGAT	4335	0.0	36.280277	7
TCAAGCG	770	0.0	36.233765	17
GACCGAT	810	0.0	36.11111	9
TTGGGAT	6450	0.0	35.93023	5
CGACCAA	730	0.0	35.753426	29
ACACGAC	750	0.0	35.7	26
>>END_MODULE