FastQCFastQC Report
Sat 14 Jan 2017
SRR2933738.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933738.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2641502
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1098944.160284565372277No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGCC51390.1945484046576531No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGC29080.11008888125013723No Hit
CTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGCCGTCTTCTGCT27710.10490243808257574No Hit
CCTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGCCGTCTTCTGC27700.1048645808331775No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT579000.044.347151
GTTTTTT652550.039.890052
TACGGGA3400.037.720594
ACTCGCG301.140323E-437.5000041
TTGGGAC56700.036.388895
TGGGCGA44150.036.3873186
GCCGTCG250.00210760336.01
TTGGGAT100400.035.789345
GCGATCG3900.035.192319
GGGCGAT68800.035.156257
TTGGGCG35600.035.0772485
CTATCGG458.7056105E-735.02
TTTGGGA163150.034.8636254
GCGATCT8200.034.847569
CGGGATC9050.034.8066336
TGCGGGA34750.034.5755424
GCGATGT9100.034.1208769
TTTGGGC68550.034.03724
TGGGATG40200.034.029856
GGCGATT32350.034.0108228
Produced by FastQC (version 0.11.5)