FastQCFastQC Report
Sat 14 Jan 2017
SRR2933737.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933737.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2662002
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1091174.099057776816096No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGCC51610.19387663871026392No Hit
CTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGCCGTCTTCTGCT28660.1076633300801427No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGC28260.10616070160728655No Hit
CCTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGCCGTCTTCTGC27290.10251682756061041No Hit
CGCTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGCCGTCTTCTG26800.1006761076813616No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT594950.044.33441
GTTTTTT678050.039.4782872
CGGTAAC301.1403253E-437.5000046
TCGTTTG8100.036.9444431
TGGGCGA43000.036.6279076
TCTTGCG7350.036.4285741
GCGATAG1050.036.428579
TTGGGAC59850.036.1654135
TACGGGA3500.036.04
TAGGGCG2650.035.6603785
CGTTTGG14600.035.599312
TTGGGCG34100.035.4985355
TTGGGAT98900.035.1263925
TTTGGGA163800.034.780224
ATGGGCG3950.034.7468385
TCTGGGA73000.034.705484
GGGCGAT67350.034.4432077
TTTTGCG19050.034.3700751
GCCGATT4200.034.2857139
GTTGGGA42450.034.2402844
Produced by FastQC (version 0.11.5)