##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933737.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2662002
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.41274536983819	34.0	31.0	34.0	31.0	34.0
2	32.63015467306185	34.0	31.0	34.0	31.0	34.0
3	32.70018204343948	34.0	31.0	34.0	31.0	34.0
4	36.14033648359393	37.0	35.0	37.0	35.0	37.0
5	36.1965152543086	37.0	35.0	37.0	35.0	37.0
6	36.18868505733655	37.0	36.0	37.0	35.0	37.0
7	36.19470158174186	37.0	37.0	37.0	35.0	37.0
8	36.13452206271821	37.0	37.0	37.0	35.0	37.0
9	37.9709523133341	39.0	39.0	39.0	35.0	39.0
10	37.44968711518624	39.0	37.0	39.0	34.0	39.0
11	37.32592800456198	39.0	37.0	39.0	34.0	39.0
12	37.394360710472796	39.0	37.0	39.0	34.0	39.0
13	37.455003790380324	39.0	37.0	39.0	35.0	39.0
14	38.78789384831416	40.0	38.0	41.0	35.0	41.0
15	38.82776421655581	40.0	38.0	41.0	35.0	41.0
16	38.895412926060914	40.0	38.0	41.0	35.0	41.0
17	38.87289040353839	40.0	38.0	41.0	35.0	41.0
18	38.52015250176371	39.0	38.0	41.0	35.0	41.0
19	38.1665043827916	39.0	37.0	41.0	35.0	41.0
20	37.65378651105446	39.0	35.0	41.0	34.0	41.0
21	37.59195372505355	39.0	35.0	41.0	34.0	41.0
22	37.568573952987265	39.0	35.0	41.0	34.0	41.0
23	37.422771282666204	39.0	35.0	41.0	34.0	41.0
24	37.34610191878143	39.0	35.0	41.0	34.0	41.0
25	37.29848287116238	39.0	35.0	41.0	33.0	41.0
26	37.26473045474797	39.0	35.0	41.0	33.0	41.0
27	37.186693698952894	39.0	35.0	41.0	33.0	41.0
28	37.10341765333009	39.0	35.0	41.0	33.0	41.0
29	36.92640952185611	38.0	35.0	41.0	33.0	41.0
30	36.68812645520176	38.0	35.0	41.0	33.0	41.0
31	36.340725889762666	38.0	35.0	41.0	31.0	41.0
32	35.866005359875764	38.0	35.0	41.0	29.0	41.0
33	35.104064534887655	38.0	35.0	41.0	21.0	41.0
34	34.50133433408389	39.0	35.0	41.0	18.0	41.0
35	34.13206150859391	39.0	34.0	41.0	15.0	41.0
36	33.89672659900331	39.0	34.0	41.0	12.0	41.0
37	33.7663213626436	39.0	34.0	41.0	10.0	41.0
38	33.64491687083631	38.0	34.0	41.0	10.0	41.0
39	33.5785033219359	39.0	33.0	41.0	10.0	41.0
40	33.44737306733804	38.0	33.0	41.0	10.0	41.0
41	33.308910737106885	38.0	33.0	41.0	10.0	41.0
42	33.10716070085597	38.0	33.0	41.0	10.0	41.0
43	33.00086588965748	38.0	33.0	41.0	10.0	41.0
44	32.863874632701254	38.0	32.0	40.0	9.0	41.0
45	32.820006897064694	38.0	32.0	40.0	9.0	41.0
46	32.67686688439753	38.0	32.0	40.0	8.0	41.0
47	32.6546659243682	38.0	32.0	40.0	8.0	41.0
48	32.53397104885721	38.0	31.0	40.0	8.0	41.0
49	32.50702441245348	38.0	31.0	40.0	8.0	41.0
50	32.35962369675154	37.0	31.0	40.0	8.0	41.0
51	31.579655837974578	36.0	29.0	40.0	8.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	11.0
10	11.0
11	20.0
12	20.0
13	27.0
14	38.0
15	76.0
16	154.0
17	324.0
18	700.0
19	1525.0
20	2776.0
21	4546.0
22	7475.0
23	13134.0
24	25723.0
25	54750.0
26	91184.0
27	101728.0
28	88052.0
29	71040.0
30	62062.0
31	61082.0
32	65602.0
33	79741.0
34	117063.0
35	151491.0
36	198339.0
37	255267.0
38	493403.0
39	714297.0
40	338.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	17.948145794030207	3.5734007712991946	44.33377585741859	34.14467757725201
2	37.6008357619566	3.4734008464306187	43.34899072202049	15.576772669592284
3	15.93529982321576	3.7031151742185022	64.11685641107707	16.24472859148866
4	13.863287856282602	3.8936860302884817	63.9452562394769	18.29776987395201
5	13.654948418521098	4.670770345026036	63.19307799167694	18.481203244775923
6	17.0593410523358	5.408636056622046	64.26569176131348	13.266331129728679
7	66.84532919208927	1.5146119349271714	28.001293763115132	3.6387651098684373
8	69.1133590433065	1.763109118625756	26.100017956410248	3.023513881657489
9	63.584700537415074	4.678846973067639	27.8950579300842	3.841394559433088
10	27.074059298227425	26.039424463242327	34.59024448516568	12.296271753364572
11	19.02327646635878	20.115461971854266	44.90146889446364	15.959792667323317
12	16.101866189431863	19.038453013934625	47.34196292865295	17.517717867980565
13	16.951039105154692	18.35964060132186	49.287491143883436	15.401829149640008
14	16.292136519807272	20.063809118099837	47.918559039399675	15.72549532269322
15	15.086126907492933	20.55374113167458	48.67956522947766	15.680566731354823
16	16.693939373449005	20.837888175891678	46.82366880265304	15.644503648006275
17	16.960205138839115	20.580525484203243	45.938169843598914	16.521099533358726
18	16.88586259514456	20.059639324087662	46.46033323791642	16.59416484285136
19	16.967906109762502	22.026279469361782	44.88302412995933	16.122790290916384
20	17.875493707367614	22.828833336714247	43.47926861061712	15.816404345301018
21	18.144764729703432	22.756256381475296	43.99865214225985	15.100326746561423
22	17.705621558511226	21.102876707079858	43.80004222386009	17.391459510548827
23	15.376284465601453	23.12943416270912	43.74545924458359	17.74882212710584
24	15.26381272440817	22.66696268447582	44.57795298425771	17.491271606858298
25	16.10746348049325	23.61256678244419	43.04068892510223	17.239280811960324
26	16.048936101475505	23.611026588259513	43.101169721134696	17.238867589130287
27	15.984473339989977	22.597428551894403	43.88572961252471	17.532368495590912
28	15.814751453980877	22.46125284654181	43.746172993108196	17.977822706369114
29	16.43090425927554	21.52766977635629	43.05556494698351	18.98586101738466
30	17.417192023146487	21.23007420730713	42.9140924762641	18.438641293282274
31	16.528838069993938	22.81020074365083	41.62645257216185	19.034508614193378
32	16.37902601125018	23.12763100854169	40.96574683264701	19.52759614756112
33	16.864976059371855	23.361402433206287	38.956882827285625	20.81673868013623
34	16.362271703777832	24.19303216150852	38.11383312258969	21.330863012123956
35	16.24367675155766	24.459485755457735	37.24080597986027	22.05603151312433
36	16.79093404137187	25.183715113662576	36.037914321627106	21.98743652333845
37	16.500701351839705	26.211738383367106	36.30470600698271	20.982854257810473
38	16.833834084271913	25.787283405497064	35.939417025231386	21.439465484999637
39	17.194201957774638	25.124060763290185	35.95414278426537	21.727594494669802
40	18.219933719058062	24.713429967370423	36.536786974615346	20.52984933895617
41	17.496756200784223	25.01703605031101	36.209589624650924	21.27661812425385
42	17.9281232696294	25.89900383245392	36.03472123612229	20.138151661794392
43	17.747544892903914	25.52191921719067	36.70433004933881	20.02620584056661
44	18.4286863796496	26.019589767400625	35.304781889720594	20.24694196322918
45	18.656860513252806	25.02515024406443	34.94629230180894	21.371696940873825
46	18.71797992638623	25.40306130498775	34.84362521140104	21.035333557224973
47	17.955884330665416	25.801070021735523	35.32116054007473	20.92188510752434
48	17.97421639803426	25.258245485916238	35.64520237024615	21.122335745803348
49	18.871548556312128	24.538674275977254	35.46894405038013	21.12083311733049
50	17.862157879671013	24.399793839373523	35.89400007963931	21.844048201316152
51	17.725944608606607	23.76512113815091	35.53637450309955	22.97255975014294
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1036.0
1	2655.0
2	4274.0
3	87190.0
4	170106.0
5	111541.0
6	52976.0
7	51019.5
8	49063.0
9	49304.0
10	49545.0
11	49024.0
12	48503.0
13	46670.0
14	44837.0
15	43028.5
16	41220.0
17	38980.5
18	36741.0
19	34492.5
20	32244.0
21	31390.5
22	30537.0
23	29940.5
24	29344.0
25	30685.5
26	33984.5
27	35942.0
28	38930.5
29	41919.0
30	47268.5
31	52618.0
32	58397.0
33	64176.0
34	73200.0
35	82224.0
36	88017.0
37	93810.0
38	100327.5
39	106845.0
40	113608.5
41	120372.0
42	129853.5
43	139335.0
44	151712.0
45	164089.0
46	181345.5
47	198602.0
48	208159.5
49	217717.0
50	208225.0
51	198733.0
52	173110.5
53	147488.0
54	127377.0
55	107266.0
56	93218.5
57	79171.0
58	69236.5
59	59302.0
60	51662.0
61	44022.0
62	37329.0
63	30636.0
64	25951.0
65	21266.0
66	17178.5
67	13091.0
68	10559.0
69	8027.0
70	6356.5
71	4686.0
72	3987.0
73	3288.0
74	2768.0
75	1831.5
76	1415.0
77	1063.5
78	712.0
79	519.0
80	326.0
81	239.0
82	152.0
83	93.0
84	34.0
85	33.0
86	32.0
87	17.5
88	3.0
89	2.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2662002.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.04366971892611
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.65786233941026	27.285575247032394
2	8.406415416574275	6.2280895242333205
3	3.8176535037432866	4.2425968648190215
4	2.530339382815968	3.749322254953042
5	1.813389177787076	3.3587294886909715
6	1.4366915120286434	3.1932195515744173
7	1.1620755886325502	3.013328100561158
8	1.0061488024497094	2.9817155140832052
9	0.8103050197413145	2.7015004370596634
>10	5.214691165462041	30.658539148105156
>50	0.08319555240868412	2.121776212479609
>100	0.05612653401440299	3.9689631782331043
>500	0.0027572426632105	0.6890179898657706
>1k	0.002144522071383283	1.4846003630315459
>5k	1.0212009863729918E-4	0.19523563444020403
>10k+	1.0212009863729918E-4	4.127790490837385
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	109117	4.099057776816096	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGCC	5161	0.19387663871026392	No Hit
CTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGCCGTCTTCTGCT	2866	0.1076633300801427	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGC	2826	0.10616070160728655	No Hit
CCTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGCCGTCTTCTGC	2729	0.10251682756061041	No Hit
CGCTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGCCGTCTTCTG	2680	0.1006761076813616	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.756571182140359E-4	0.0	0.0	0.1161156152399585	0.0
2	3.756571182140359E-4	0.0	0.0	0.3223138074276428	0.0
3	3.756571182140359E-4	0.0	0.0	0.5574375977178079	0.0
4	4.5078854185684307E-4	0.0	0.0	0.8504877156365773	0.0
5	4.883542536782467E-4	0.0	0.0	1.4945142791027204	0.0
6	4.883542536782467E-4	0.0	0.0	2.626594570552539	0.0
7	4.883542536782467E-4	0.0	0.0	3.678847724381875	0.0
8	4.883542536782467E-4	0.0	0.0	5.112054761792065	0.0
9	5.259199654996503E-4	0.0	0.0	6.1438721683905575	0.0
10	5.259199654996503E-4	0.0	0.0	7.260437820858136	0.0
11	5.259199654996503E-4	0.0	0.0	8.70284845766457	0.0
12	5.634856773210538E-4	0.0	0.0	9.863441124386833	0.0
13	5.634856773210538E-4	0.0	0.0	10.466934284797683	0.0
14	5.634856773210538E-4	0.0	0.0	10.804837862631208	0.0
15	6.010513891424574E-4	0.0	0.0	11.065280942688998	0.0
16	6.010513891424574E-4	0.0	0.0	11.44961574033378	0.0
17	6.38617100963861E-4	0.0	0.0	11.919562795219537	0.0
18	6.761828127852647E-4	0.0	0.0	12.429630030330555	0.0
19	6.761828127852647E-4	0.0	0.0	12.756301460329482	0.0
20	6.761828127852647E-4	0.0	0.0	13.084099861683049	0.0
21	6.761828127852647E-4	0.0	0.0	13.426586456358786	0.0
22	7.137485246066682E-4	0.0	0.0	13.754197029153246	0.0
23	7.137485246066682E-4	0.0	0.0	14.069298219911179	0.0
24	7.137485246066682E-4	0.0	0.0	14.324970454567653	0.0
25	7.137485246066682E-4	0.0	0.0	14.556826027929356	0.0
26	7.137485246066682E-4	0.0	0.0	14.776848402067316	0.0
27	7.137485246066682E-4	0.0	0.0	15.020161517534547	0.0
28	7.137485246066682E-4	0.0	0.0	15.256337147755712	0.0
29	7.137485246066682E-4	0.0	0.0	15.50629939421533	0.0
30	7.137485246066682E-4	0.0	0.0	15.784999410218324	0.0
31	7.137485246066682E-4	0.0	0.0	16.03135534834309	0.0
32	7.137485246066682E-4	0.0	0.0	16.293526451144665	0.0
33	7.137485246066682E-4	0.0	0.0	16.539732126422145	0.0
34	7.513142364280718E-4	0.0	0.0	16.794916006824938	0.0
35	7.513142364280718E-4	0.0	0.0	17.067868468919258	0.0
36	8.26445660070879E-4	0.0	0.0	17.32248886364473	0.0
37	8.26445660070879E-4	0.0	0.0	17.59063291462591	0.0
38	8.26445660070879E-4	0.0	0.0	17.867567342173295	0.0
39	8.26445660070879E-4	0.0	0.0	18.21587662218135	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	59495	0.0	44.3344	1
GTTTTTT	67805	0.0	39.478287	2
CGGTAAC	30	1.1403253E-4	37.500004	6
TCGTTTG	810	0.0	36.944443	1
TGGGCGA	4300	0.0	36.627907	6
TCTTGCG	735	0.0	36.428574	1
GCGATAG	105	0.0	36.42857	9
TTGGGAC	5985	0.0	36.165413	5
TACGGGA	350	0.0	36.0	4
TAGGGCG	265	0.0	35.660378	5
CGTTTGG	1460	0.0	35.59931	2
TTGGGCG	3410	0.0	35.498535	5
TTGGGAT	9890	0.0	35.126392	5
TTTGGGA	16380	0.0	34.78022	4
ATGGGCG	395	0.0	34.746838	5
TCTGGGA	7300	0.0	34.70548	4
GGGCGAT	6735	0.0	34.443207	7
TTTTGCG	1905	0.0	34.370075	1
GCCGATT	420	0.0	34.285713	9
GTTGGGA	4245	0.0	34.240284	4
>>END_MODULE