FastQCFastQC Report
Sat 14 Jan 2017
SRR2933736.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933736.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1575964
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT739924.695031104771428No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCC28570.18128586693604676No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA17030.10806084402943214No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGC16110.10222314722925144No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTT207.034085E-445.016
GTTAGCG207.034085E-445.01
CGGTGCG207.034085E-445.01
CGTTTTT467800.044.3554951
AGCTACG1850.041.3513539
GTTTTTT525850.040.032332
GCGATGC4100.039.5121969
GCTATCG403.4593177E-739.3751
GGACCGT403.4593177E-739.3758
TTAGCGG1950.039.230772
TACGGGA3000.039.04
TACGCGG356.2497656E-638.5714262
CCTATGC1700.038.3823535
CTTTGCG4350.037.758621
GCTAGCG301.14011425E-437.4999961
CTAGCGA301.14011425E-437.49999628
TGGGCGA24850.036.941656
TCGCTCG552.750312E-936.818181
GGGACCG8100.036.6666687
TTGGGCG19500.036.5769275
Produced by FastQC (version 0.11.5)