##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933736.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1575964
Sequences flagged as poor quality	0
Sequence length	51
%GC	35
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.62224771631839	34.0	31.0	34.0	31.0	34.0
2	32.81854344388577	34.0	31.0	34.0	31.0	34.0
3	32.84243104537921	34.0	31.0	34.0	31.0	34.0
4	36.302815927267375	37.0	37.0	37.0	35.0	37.0
5	36.31690063986233	37.0	37.0	37.0	35.0	37.0
6	36.299028404202126	37.0	37.0	37.0	35.0	37.0
7	36.38205250881365	37.0	37.0	37.0	35.0	37.0
8	36.31647994497336	37.0	37.0	37.0	35.0	37.0
9	38.16987761141752	39.0	39.0	39.0	37.0	39.0
10	37.67244556347734	39.0	38.0	39.0	35.0	39.0
11	37.53346015518121	39.0	37.0	39.0	35.0	39.0
12	37.605753050196576	39.0	37.0	39.0	35.0	39.0
13	37.64707886728377	39.0	37.0	39.0	35.0	39.0
14	39.05953943110376	40.0	39.0	41.0	35.0	41.0
15	39.092841587752005	40.0	39.0	41.0	35.0	41.0
16	39.08669360467625	40.0	39.0	41.0	35.0	41.0
17	39.005610534250785	40.0	38.0	41.0	35.0	41.0
18	38.48132508102977	39.0	38.0	41.0	35.0	41.0
19	37.944473985446365	38.0	37.0	41.0	35.0	41.0
20	37.354196542560615	38.0	35.0	41.0	34.0	41.0
21	37.273214362764634	38.0	35.0	41.0	34.0	41.0
22	37.2666977164453	38.0	35.0	41.0	34.0	41.0
23	37.16178351789762	38.0	35.0	40.0	34.0	41.0
24	37.064833333756354	38.0	35.0	40.0	34.0	41.0
25	37.00261681104391	38.0	35.0	40.0	33.0	41.0
26	36.959756060417625	38.0	35.0	40.0	33.0	41.0
27	36.892006416390224	38.0	35.0	40.0	33.0	41.0
28	36.74166605328548	38.0	35.0	40.0	33.0	41.0
29	36.59224449289451	38.0	35.0	40.0	33.0	41.0
30	36.33828120439299	38.0	35.0	40.0	33.0	41.0
31	35.857500552043064	38.0	35.0	40.0	31.0	41.0
32	35.04281696790028	38.0	35.0	40.0	23.0	41.0
33	33.997499308359835	38.0	34.0	40.0	16.0	41.0
34	33.076707335954374	38.0	33.0	41.0	10.0	41.0
35	32.454632846943205	38.0	31.0	41.0	8.0	41.0
36	32.133404062529344	38.0	31.0	40.0	7.0	41.0
37	31.904018112088856	38.0	30.0	40.0	7.0	41.0
38	31.723399138559003	38.0	29.0	40.0	7.0	41.0
39	31.591051572244037	37.0	27.0	40.0	7.0	41.0
40	31.425188646441164	37.0	26.0	40.0	7.0	41.0
41	31.27871448840202	37.0	25.0	40.0	7.0	41.0
42	31.1223238601897	37.0	24.0	40.0	7.0	41.0
43	30.944458122139846	37.0	23.0	40.0	7.0	41.0
44	30.768899543390585	37.0	23.0	40.0	7.0	41.0
45	30.741817706495834	36.0	23.0	40.0	7.0	41.0
46	30.619292699579432	36.0	23.0	40.0	7.0	41.0
47	30.53659982080809	36.0	23.0	40.0	7.0	41.0
48	30.445442916208744	36.0	23.0	40.0	7.0	41.0
49	30.39339858017061	36.0	23.0	40.0	7.0	41.0
50	30.291884205476777	35.0	23.0	40.0	7.0	41.0
51	29.547636240421735	35.0	20.0	40.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	2.0
10	1.0
11	5.0
12	9.0
13	13.0
14	14.0
15	38.0
16	90.0
17	217.0
18	551.0
19	1110.0
20	2097.0
21	3508.0
22	5881.0
23	10251.0
24	21022.0
25	45467.0
26	75954.0
27	84223.0
28	70267.0
29	53714.0
30	43310.0
31	39134.0
32	38776.0
33	43996.0
34	62920.0
35	83401.0
36	107732.0
37	138135.0
38	268961.0
39	375024.0
40	138.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.125667845204585	3.333578685807544	38.35753862397872	40.183214845009154
2	44.19555269028988	3.2066722336297024	39.53960877278922	13.058166303291191
3	15.105801909180666	3.6377099984517414	67.90783292004133	13.348655172326271
4	12.621037028764617	3.6594110017741523	67.62806764621527	16.091484323245965
5	12.668246228974771	4.183534649268638	67.0260234370836	16.122195684673
6	15.320400719813396	4.885010063681658	67.63549167366767	12.159097542837273
7	61.90154089814235	1.2391780522905345	34.48118104220655	2.3781000073605743
8	64.23116264077098	1.4264919757050287	32.599158356409156	1.7431870271148324
9	59.98411131218734	3.9934287839062312	33.67062953214667	2.3518303717597613
10	23.749210007335193	27.81884611577422	38.77157092420893	9.66037295268166
11	17.40515646296489	20.358840684178066	49.390722123094186	12.845280729762862
12	15.298636263264898	19.90229472246828	50.850336682817634	13.948732331449195
13	15.505557233540868	19.4319794106972	51.932785266668525	13.129678089093405
14	15.536966580454884	20.214928767408395	50.849702150556745	13.398402501579984
15	14.463909074065143	19.744867268541665	51.521798721290594	14.2694249361026
16	16.290156374130373	20.08218461843037	50.41301704861279	13.214641958826471
17	16.311857377452785	19.57119578873629	50.262125276973336	13.854821556837592
18	17.335992446527968	17.95358269605143	50.524948539433645	14.185476317986959
19	16.39904211009896	20.10344144917016	49.15385123010424	14.343665210626638
20	18.11316755966507	20.9399453287004	48.20770017589234	12.739186935742186
21	17.929280110459374	21.027447327477024	48.16436162247361	12.878910939589991
22	17.727625757948786	18.771304420659355	48.800289854336775	14.700779967055086
23	15.451431631687019	21.53602493457972	48.34164993616605	14.670893497567203
24	14.386242325332304	21.801069059953146	49.47359203636631	14.339096578348236
25	14.954148698828146	23.194755717770203	47.97558827485907	13.87550730854258
26	15.713620361886438	22.564221010124598	47.553878134272104	14.168280493716862
27	14.89221835016536	21.969854641349677	48.61132614704397	14.526600861440997
28	13.828044295428068	22.37887413671886	48.7886779139625	15.004403653890572
29	15.144635283547087	20.746349535903104	48.66367505856733	15.44534012198248
30	16.41573030856035	20.433969303867347	48.829097618981145	14.32120276859116
31	16.668083788715986	22.215735892444243	46.5218114119358	14.594368906903965
32	16.404752900446965	22.513521882479548	45.85866174608049	15.223063470992992
33	17.002482290204597	23.823640641537498	42.910624861989234	16.26325220626867
34	17.247284836455655	25.346454614445506	41.361795066384765	16.04446548271407
35	17.3816153160859	26.861019667961962	39.50344043391854	16.253924582033598
36	18.75106284153699	27.809455038313057	37.44552540540266	15.99395671474729
37	18.963504242482696	27.774365404285884	36.72621963445866	16.535910718772765
38	19.458312499524098	28.450459528263334	36.1635798787282	15.92764809348437
39	20.117020439553187	27.619031906820208	35.19839285668962	17.065554796936986
40	20.920845907647635	27.845115751375033	35.14198293869657	16.092055402280764
41	19.80406912848263	27.5027855966253	35.1498511387316	17.54329413616047
42	20.481305410529682	27.422834531753264	35.037475475328115	17.05838458238894
43	19.733064968489128	26.87618498899721	35.658682558738654	17.732067483775012
44	19.726148566845435	27.69390671360513	34.74743077887566	17.83251394067377
45	20.01118045843687	26.927011023094437	34.793941993598835	18.26786652486986
46	19.63636225192961	27.247132548713044	34.77833249998096	18.338172699376383
47	18.65359868626441	28.138777281714557	35.53006286945641	17.67756116256463
48	19.223281750090738	27.48470142718996	35.656588602277715	17.635428220441586
49	19.79302826714316	26.711841133426905	35.3061998878147	18.18893071161524
50	18.83164843866992	26.943826128008002	35.213177458368335	19.011347974953743
51	18.55873611326147	25.99145665763939	34.826239685678104	20.623567543421043
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	611.0
1	1927.0
2	3243.0
3	62728.0
4	122213.0
5	82134.5
6	42056.0
7	41305.0
8	40554.0
9	40672.0
10	40790.0
11	40233.0
12	39676.0
13	38406.5
14	37137.0
15	35244.0
16	33351.0
17	31586.5
18	29822.0
19	28081.0
20	26340.0
21	24913.0
22	23486.0
23	22734.0
24	21982.0
25	21978.5
26	22375.0
27	22775.0
28	24364.0
29	25953.0
30	28403.5
31	30854.0
32	35158.0
33	39462.0
34	44104.5
35	48747.0
36	52959.5
37	57172.0
38	59964.0
39	62756.0
40	67410.0
41	72064.0
42	76463.5
43	80863.0
44	83721.0
45	86579.0
46	90876.0
47	95173.0
48	96972.0
49	98771.0
50	95339.5
51	91908.0
52	84474.0
53	77040.0
54	69323.0
55	61606.0
56	52561.5
57	43517.0
58	38055.5
59	32594.0
60	27742.5
61	22891.0
62	18958.0
63	15025.0
64	12235.0
65	9445.0
66	7413.0
67	5381.0
68	4236.0
69	3091.0
70	2501.0
71	1911.0
72	1963.5
73	2016.0
74	2114.0
75	1927.5
76	1643.0
77	1171.5
78	700.0
79	518.5
80	337.0
81	240.0
82	143.0
83	97.5
84	52.0
85	40.0
86	28.0
87	21.0
88	14.0
89	8.5
90	3.0
91	2.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1575964.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.796914274171876
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.12842591551754	29.535480140719788
2	7.895570009713982	6.126475056251915
3	3.5729996736356893	4.158640861190639
4	2.2164983346848315	3.4397318351844857
5	1.6145070719679866	3.1318946233093117
6	1.2441007297428022	2.896036161615971
7	0.9956011764315287	2.7038377445295083
8	0.8190328473451205	2.5420757732943637
9	0.6879509312361712	2.4021335973605806
>10	4.679618457004541	30.725673038975565
>50	0.0924502174485693	2.466938551075209
>100	0.04898508908776238	3.5962013446816843
>500	0.0026212591898068798	0.7038051955751218
>1k	0.0014744582942663698	0.8681090365000464
>5k	0.0	0.0
>10k+	1.6382869936292999E-4	4.702967039735792
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	73992	4.695031104771428	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCC	2857	0.18128586693604676	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	1703	0.10806084402943214	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGC	1611	0.10222314722925144	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2690645217784162E-4	0.0	0.0	0.06167653575843103	0.0
2	1.2690645217784162E-4	0.0	0.0	0.18953478632760648	0.0
3	1.2690645217784162E-4	0.0	0.0	0.3121898723574904	0.0
4	1.2690645217784162E-4	0.0	0.0	0.4585764649446307	0.0
5	1.2690645217784162E-4	0.0	0.0	0.7750811566761677	0.0
6	1.2690645217784162E-4	0.0	0.0	1.308532428405725	0.0
7	1.2690645217784162E-4	0.0	0.0	1.7855103289161427	0.0
8	1.2690645217784162E-4	0.0	0.0	2.4347002850318917	0.0
9	1.2690645217784162E-4	0.0	0.0	2.8691645240627324	0.0
10	1.9035967826676244E-4	0.0	0.0	3.459469886367963	0.0
11	1.9035967826676244E-4	0.0	0.0	4.367295192022153	0.0
12	1.9035967826676244E-4	0.0	0.0	5.090598516209761	0.0
13	1.9035967826676244E-4	0.0	0.0	5.405453424062986	0.0
14	1.9035967826676244E-4	0.0	0.0	5.556725915058974	0.0
15	1.9035967826676244E-4	0.0	0.0	5.702795241515669	0.0
16	1.9035967826676244E-4	0.0	0.0	5.971963826584871	0.0
17	1.9035967826676244E-4	0.0	0.0	6.345005342761636	0.0
18	2.5381290435568324E-4	0.0	0.0	6.722552037990716	0.0
19	2.5381290435568324E-4	0.0	0.0	6.964435735841682	0.0
20	2.5381290435568324E-4	0.0	0.0	7.213235835336341	0.0
21	2.5381290435568324E-4	0.0	0.0	7.4751707526314055	0.0
22	2.5381290435568324E-4	0.0	0.0	7.739389986065672	0.0
23	3.172661304446041E-4	0.0	0.0	7.982733108116683	0.0
24	3.172661304446041E-4	0.0	0.0	8.16160775246135	0.0
25	3.172661304446041E-4	0.0	0.0	8.316433624118318	0.0
26	3.172661304446041E-4	0.0	0.0	8.4574266924879	0.0
27	3.807193565335249E-4	0.0	0.0	8.609206809292598	0.0
28	3.807193565335249E-4	0.0	0.0	8.761240739001652	0.0
29	4.441725826224457E-4	0.0	0.0	8.93357970105916	0.0
30	4.441725826224457E-4	0.0	0.0	9.143736785865666	0.0
31	4.441725826224457E-4	0.0	0.0	9.319502222131977	0.0
32	4.441725826224457E-4	0.0	0.0	9.513224921381452	0.0
33	4.441725826224457E-4	0.0	0.0	9.686959854412917	0.0
34	5.076258087113665E-4	0.0	0.0	9.844323855113442	0.0
35	5.076258087113665E-4	0.0	0.0	10.029924541423535	0.0
36	5.076258087113665E-4	0.0	0.0	10.194585663124284	0.0
37	5.076258087113665E-4	0.0	0.0	10.364132683233882	0.0
38	5.076258087113665E-4	0.0	0.0	10.568071351883672	0.0
39	5.076258087113665E-4	0.0	0.0	10.814777494917397	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGTT	20	7.034085E-4	45.0	16
GTTAGCG	20	7.034085E-4	45.0	1
CGGTGCG	20	7.034085E-4	45.0	1
CGTTTTT	46780	0.0	44.355495	1
AGCTACG	185	0.0	41.351353	9
GTTTTTT	52585	0.0	40.03233	2
GCGATGC	410	0.0	39.512196	9
GCTATCG	40	3.4593177E-7	39.375	1
GGACCGT	40	3.4593177E-7	39.375	8
TTAGCGG	195	0.0	39.23077	2
TACGGGA	300	0.0	39.0	4
TACGCGG	35	6.2497656E-6	38.571426	2
CCTATGC	170	0.0	38.38235	35
CTTTGCG	435	0.0	37.75862	1
GCTAGCG	30	1.14011425E-4	37.499996	1
CTAGCGA	30	1.14011425E-4	37.499996	28
TGGGCGA	2485	0.0	36.94165	6
TCGCTCG	55	2.750312E-9	36.81818	1
GGGACCG	810	0.0	36.666668	7
TTGGGCG	1950	0.0	36.576927	5
>>END_MODULE