FastQCFastQC Report
Sat 14 Jan 2017
SRR2933735.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933735.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1586699
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT730644.604780112674175No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCC28470.17942911667556355No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA17530.1104809418799659No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGC16510.1040525014511259No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCACT207.034098E-445.00000418
CGTTTTT458000.044.4006541
GTTTTTT518850.039.887252
TACGGGA2500.039.64
TTACGCG403.4593359E-739.3750041
CGAATAT1500.039.00000414
AGCTACG1500.039.0000049
TCTCGCG601.5643309E-1037.4999961
AGTCGAC301.140118E-437.49999623
AGACACG8400.037.23214324
GTTTGCG2850.037.105261
TTTTGCG11600.036.8534471
CTCGTTG1650.036.818181
TGGGCGA26450.036.7485856
CTTTGCG4300.036.6279071
GACACGA8400.036.42857425
CACGACC8400.036.42857427
TTGGGAC37100.036.327495
TTGGGCG20050.036.2468835
ACACGAC8450.036.2130226
Produced by FastQC (version 0.11.5)