##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933735.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1586699
Sequences flagged as poor quality	0
Sequence length	51
%GC	35
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.546234667066656	34.0	31.0	34.0	31.0	34.0
2	32.764910673038806	34.0	31.0	34.0	31.0	34.0
3	32.796562549040495	34.0	31.0	34.0	31.0	34.0
4	36.23304798200541	37.0	37.0	37.0	35.0	37.0
5	36.289223097764605	37.0	37.0	37.0	35.0	37.0
6	36.2909228530427	37.0	37.0	37.0	35.0	37.0
7	36.31145667829878	37.0	37.0	37.0	35.0	37.0
8	36.27697565826915	37.0	37.0	37.0	35.0	37.0
9	38.14368761813047	39.0	39.0	39.0	37.0	39.0
10	37.68115691760063	39.0	38.0	39.0	35.0	39.0
11	37.54387000937166	39.0	37.0	39.0	35.0	39.0
12	37.59123375006854	39.0	37.0	39.0	35.0	39.0
13	37.64274005340648	39.0	37.0	39.0	35.0	39.0
14	39.0726949471828	40.0	39.0	41.0	35.0	41.0
15	39.0986242507243	40.0	39.0	41.0	35.0	41.0
16	39.144685917114714	41.0	39.0	41.0	35.0	41.0
17	39.082252525526265	40.0	39.0	41.0	35.0	41.0
18	38.594893549438176	39.0	38.0	41.0	35.0	41.0
19	38.0905641208572	39.0	37.0	41.0	35.0	41.0
20	37.46044965050082	39.0	35.0	41.0	34.0	41.0
21	37.40542724234401	38.0	35.0	41.0	34.0	41.0
22	37.388008059499626	38.0	35.0	41.0	34.0	41.0
23	37.22925835334868	38.0	35.0	41.0	34.0	41.0
24	37.14347774845765	38.0	35.0	41.0	34.0	41.0
25	37.089790187048706	38.0	35.0	41.0	34.0	41.0
26	37.076787720922496	38.0	35.0	41.0	34.0	41.0
27	37.006430961385874	38.0	35.0	41.0	34.0	41.0
28	36.911357478639616	38.0	35.0	41.0	33.0	41.0
29	36.71226489712289	38.0	35.0	41.0	33.0	41.0
30	36.375086263998405	38.0	35.0	41.0	33.0	41.0
31	35.89891088353872	38.0	35.0	41.0	31.0	41.0
32	35.16626846049566	38.0	35.0	41.0	23.0	41.0
33	34.079615604471925	38.0	34.0	41.0	16.0	41.0
34	33.18565588054193	38.0	33.0	41.0	12.0	41.0
35	32.61111590793213	38.0	32.0	41.0	8.0	41.0
36	32.27759329274172	38.0	31.0	41.0	8.0	41.0
37	32.06830280979568	38.0	30.0	41.0	7.0	41.0
38	31.883009316826946	38.0	30.0	41.0	7.0	41.0
39	31.781215592875522	38.0	29.0	41.0	7.0	41.0
40	31.61703511504072	38.0	27.0	41.0	7.0	41.0
41	31.467499506837783	37.0	26.0	40.0	7.0	41.0
42	31.25787625756366	37.0	24.0	40.0	7.0	41.0
43	31.122518511702598	37.0	24.0	40.0	7.0	41.0
44	30.94871175944524	37.0	23.0	40.0	7.0	41.0
45	30.90095853088708	37.0	23.0	40.0	7.0	41.0
46	30.758375091936152	36.0	23.0	40.0	7.0	41.0
47	30.72955299020167	36.0	23.0	40.0	7.0	41.0
48	30.6051614074251	36.0	23.0	40.0	7.0	41.0
49	30.541219853292905	36.0	23.0	40.0	7.0	41.0
50	30.389341015529727	36.0	23.0	40.0	7.0	41.0
51	29.73396907668058	35.0	22.0	40.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	4.0
10	9.0
11	2.0
12	15.0
13	15.0
14	15.0
15	41.0
16	96.0
17	228.0
18	506.0
19	1090.0
20	2028.0
21	3495.0
22	5481.0
23	10091.0
24	20652.0
25	45220.0
26	75347.0
27	82881.0
28	69987.0
29	53777.0
30	43277.0
31	38955.0
32	38509.0
33	43234.0
34	62312.0
35	82885.0
36	106260.0
37	133540.0
38	263894.0
39	402626.0
40	224.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.235846874548987	3.4719880708313298	38.359197302071784	39.932967752547896
2	44.11025657670422	3.2453540337518327	39.54858482925873	13.09580456028522
3	15.30006636419384	3.7385162529250984	67.57015665857229	13.39126072430877
4	12.846860053482104	3.7242098217746404	67.33249343448253	16.096436690260724
5	12.752387188748465	4.219892997978823	66.87645230758953	16.15126750568318
6	15.38174537199557	4.894753195155477	67.49093558387571	12.232565848973245
7	61.999408835576254	1.2903518562751979	34.24663404968428	2.4636052584642707
8	64.38650304815216	1.4586257380889507	32.380558631473264	1.7743125822856132
9	60.17398384948878	4.021304607868284	33.422155052722665	2.3825564899202685
10	23.795943654089402	27.923065433330457	38.588478344033746	9.692512568546398
11	17.571952840456824	20.395802858639225	49.18475400816412	12.847490292739833
12	15.335863954032869	20.000453772265566	50.6889460445869	13.97473622911466
13	15.574976728415407	19.496451437859356	51.74812614112696	13.18044569259828
14	15.60415680604828	20.347400483645607	50.616405506022254	13.432037204283862
15	14.495691999553792	19.843587221016715	51.39834335308713	14.262377426342363
16	16.391955878210045	20.16702600808345	50.141457201397365	13.299560912309142
17	16.490714369896246	19.601953489603257	50.121352569075796	13.7859795714247
18	17.487311708143764	18.016649660710694	50.322146796588385	14.173891834557153
19	16.389687016882217	20.172004898219512	48.993728489146335	14.444579595751936
20	18.181646298384255	20.99263943570898	48.07654129737272	12.749172968534046
21	18.038455939028132	21.07904523794368	48.00784521828022	12.874653604747971
22	17.818691509857885	18.837347222125935	48.686675922780566	14.657285345235612
23	15.557960268456714	21.648466407302205	48.158409376951774	14.635163947289309
24	14.423088437063361	21.89299923930122	49.32630574545014	14.357606578185274
25	14.994715443823939	23.29616392270998	47.87782685941064	13.831293774055444
26	15.72799882019211	22.69258378558252	47.36128276377561	14.218134630449757
27	14.971900782694133	22.111944357436414	48.4369121049424	14.479242754927052
28	13.900746140257226	22.451580293426794	48.66411335735385	14.98356020896213
29	15.184732580029356	20.907115968435097	48.5263430556142	15.381808395921343
30	16.518129777607474	20.546682136939644	48.63241232269006	14.302775762762817
31	16.765435662340494	22.29534398143567	46.27903591040267	14.660184445821168
32	16.439286846465524	22.660504607364093	45.649426891930986	15.250781654239399
33	17.08673163593095	23.858400364530386	42.78435922629308	16.270508773245588
34	17.341222248202083	25.390133856515952	41.24361331292199	16.02503058235998
35	17.430401103170798	26.909514659050014	39.369344784360486	16.290739453418702
36	18.784659220179755	27.755232718997114	37.43337583246728	16.02673222835585
37	18.922051378364767	27.849264416250342	36.78088912894002	16.44779507644487
38	19.554370425644688	28.411437834144976	36.119516051878776	15.914675688331561
39	20.1179933938321	27.704309386972575	35.18178306030318	16.995914158892138
40	20.90724201628664	27.932203902567533	35.16155238013007	15.999001701015755
41	19.81925998566836	27.626411814717223	35.10943159351584	17.444896606098574
42	20.540190672585034	27.496015312293004	34.97166129177619	16.99213272334576
43	19.779239792802542	26.941593837268442	35.592383936713894	17.68678243321512
44	19.79896628156947	27.784223724852662	34.73620390508849	17.680606088489373
45	20.00158820292948	27.04911265463708	34.67116321369082	18.27813592874263
46	19.641973682469075	27.43841144413654	34.748304498836895	18.171310374557493
47	18.689240996559526	28.252239397642526	35.53396075752238	17.52455884827557
48	19.251036270899522	27.643617346453237	35.53862452802958	17.56672185461767
49	19.813209688794156	26.853297317260548	35.26295787669873	18.07053511724656
50	18.85764092622482	27.04848241537935	35.226845167230834	18.86703149116499
51	18.5858817582919	26.091716198220333	34.85027721073751	20.472124832750257
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	658.0
1	1947.0
2	3236.0
3	62708.0
4	122180.0
5	82723.5
6	43267.0
7	42009.0
8	40751.0
9	40682.5
10	40614.0
11	40162.5
12	39711.0
13	38290.5
14	36870.0
15	35028.5
16	33187.0
17	31364.5
18	29542.0
19	27770.0
20	25998.0
21	24815.5
22	23633.0
23	22806.0
24	21979.0
25	22008.0
26	22452.0
27	22867.0
28	24560.0
29	26253.0
30	28911.5
31	31570.0
32	35797.5
33	40025.0
34	44548.0
35	49071.0
36	53439.5
37	57808.0
38	60616.0
39	63424.0
40	68158.5
41	72893.0
42	77552.5
43	82212.0
44	84826.0
45	87440.0
46	91589.5
47	95739.0
48	97282.0
49	98825.0
50	96136.5
51	93448.0
52	85505.0
53	77562.0
54	69723.0
55	61884.0
56	52981.5
57	44079.0
58	38191.0
59	32303.0
60	27617.0
61	22931.0
62	19175.5
63	15420.0
64	12480.0
65	9540.0
66	7514.5
67	5489.0
68	4354.5
69	3220.0
70	2608.5
71	1997.0
72	1980.0
73	1963.0
74	2067.0
75	1885.0
76	1599.0
77	1177.0
78	755.0
79	540.5
80	326.0
81	236.5
82	147.0
83	92.5
84	38.0
85	30.0
86	22.0
87	17.0
88	12.0
89	7.0
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1586699.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.51883307411358
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.68616807260679	29.15342874008045
2	8.00450161707809	6.166481232594063
3	3.719555067236551	4.29818762234574
4	2.3236823863683966	3.5802213583112863
5	1.6130446178658535	3.106629818833607
6	1.2614735204765328	2.915429277758998
7	0.986084321977058	2.658797217666433
8	0.8130974602341715	2.5055652274996603
9	0.6988989053502078	2.4228693243778827
>10	4.7523175491944265	31.032111487063652
>50	0.08626868386137704	2.290019096699901
>100	0.05095050415678035	3.7208389534789146
>500	0.002308421263126309	0.6341101784987011
>1k	0.001483985097724056	0.8748230767869701
>5k	0.0	0.0
>10k+	1.6488723308045067E-4	4.640487388003716
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	73064	4.604780112674175	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCC	2847	0.17942911667556355	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	1753	0.1104809418799659	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGC	1651	0.1040525014511259	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.151196288647059E-4	0.0	0.0	0.05955760985542942	0.0
2	3.151196288647059E-4	0.0	0.0	0.18604662888172238	0.0
3	3.151196288647059E-4	0.0	0.0	0.3047206811121706	0.0
4	3.151196288647059E-4	0.0	0.0	0.448982447206433	0.0
5	3.151196288647059E-4	0.0	0.0	0.7678204876917424	0.0
6	3.151196288647059E-4	0.0	0.0	1.2710035110629048	0.0
7	3.151196288647059E-4	0.0	0.0	1.7221287717456177	0.0
8	3.7814355463764706E-4	0.0	0.0	2.357094823908	0.0
9	3.7814355463764706E-4	0.0	0.0	2.7806786290279377	0.0
10	3.7814355463764706E-4	0.0	0.0	3.3636499424276436	0.0
11	3.7814355463764706E-4	0.0	0.0	4.233947333426189	0.0
12	3.7814355463764706E-4	0.0	0.0	4.955508259600593	0.0
13	4.411674804105883E-4	0.0	0.0	5.260733132118946	0.0
14	4.411674804105883E-4	0.0	0.0	5.406381424580213	0.0
15	4.411674804105883E-4	0.0	0.0	5.554298578369306	0.0
16	4.411674804105883E-4	0.0	0.0	5.810679908413631	0.0
17	4.411674804105883E-4	0.0	0.0	6.170924668131763	0.0
18	4.411674804105883E-4	0.0	0.0	6.544908643668395	0.0
19	5.041914061835295E-4	0.0	0.0	6.78370629842207	0.0
20	5.041914061835295E-4	0.0	0.0	7.016831799856179	0.0
21	5.041914061835295E-4	0.0	0.0	7.273591273455142	0.0
22	5.041914061835295E-4	0.0	0.0	7.529783531722147	0.0
23	5.672153319564706E-4	0.0	0.0	7.7565436166531905	0.0
24	5.672153319564706E-4	0.0	0.0	7.927149383720542	0.0
25	5.672153319564706E-4	0.0	0.0	8.077272374911688	0.0
26	5.672153319564706E-4	0.0	0.0	8.213278006729695	0.0
27	5.672153319564706E-4	0.0	0.0	8.361069112667241	0.0
28	5.672153319564706E-4	0.0	0.0	8.515666802588267	0.0
29	5.672153319564706E-4	0.0	0.0	8.678772722488638	0.0
30	5.672153319564706E-4	0.0	0.0	8.887823084277484	0.0
31	5.672153319564706E-4	0.0	0.0	9.051559243435586	0.0
32	5.672153319564706E-4	0.0	0.0	9.243404073488419	0.0
33	5.672153319564706E-4	0.0	0.0	9.408274663310433	0.0
34	5.672153319564706E-4	0.0	0.0	9.563565616414959	0.0
35	5.672153319564706E-4	0.0	0.0	9.751376915218325	0.0
36	6.302392577294118E-4	0.0	0.0	9.917760079258889	0.0
37	6.302392577294118E-4	0.0	0.0	10.096117789196313	0.0
38	6.302392577294118E-4	0.0	0.0	10.291744054795522	0.0
39	6.302392577294118E-4	0.0	0.0	10.526571202225501	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCACT	20	7.034098E-4	45.000004	18
CGTTTTT	45800	0.0	44.400654	1
GTTTTTT	51885	0.0	39.88725	2
TACGGGA	250	0.0	39.6	4
TTACGCG	40	3.4593359E-7	39.375004	1
CGAATAT	150	0.0	39.000004	14
AGCTACG	150	0.0	39.000004	9
TCTCGCG	60	1.5643309E-10	37.499996	1
AGTCGAC	30	1.140118E-4	37.499996	23
AGACACG	840	0.0	37.232143	24
GTTTGCG	285	0.0	37.10526	1
TTTTGCG	1160	0.0	36.853447	1
CTCGTTG	165	0.0	36.81818	1
TGGGCGA	2645	0.0	36.748585	6
CTTTGCG	430	0.0	36.627907	1
GACACGA	840	0.0	36.428574	25
CACGACC	840	0.0	36.428574	27
TTGGGAC	3710	0.0	36.32749	5
TTGGGCG	2005	0.0	36.246883	5
ACACGAC	845	0.0	36.21302	26
>>END_MODULE