##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933734.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	183328
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.004009207540584	33.0	31.0	34.0	30.0	34.0
2	32.19905851806598	34.0	31.0	34.0	30.0	34.0
3	32.325242188863676	34.0	31.0	34.0	30.0	34.0
4	35.90526815325537	37.0	35.0	37.0	35.0	37.0
5	35.93782182754408	37.0	35.0	37.0	35.0	37.0
6	35.89886978530284	37.0	35.0	37.0	35.0	37.0
7	35.975606563100015	37.0	35.0	37.0	35.0	37.0
8	35.77244065281899	37.0	35.0	37.0	35.0	37.0
9	37.5786950165823	39.0	37.0	39.0	35.0	39.0
10	37.0567561965439	39.0	37.0	39.0	33.0	39.0
11	37.14763156746378	39.0	37.0	39.0	34.0	39.0
12	37.181576191307386	39.0	37.0	39.0	34.0	39.0
13	37.127601893873276	39.0	37.0	39.0	33.0	39.0
14	38.18737454180485	40.0	37.0	41.0	33.0	41.0
15	38.227924812358175	40.0	37.0	41.0	33.0	41.0
16	38.28847748298132	40.0	38.0	41.0	33.0	41.0
17	38.25770204224123	40.0	38.0	41.0	33.0	41.0
18	38.01352221155525	39.0	37.0	40.0	34.0	41.0
19	37.71866817943795	39.0	37.0	40.0	34.0	41.0
20	37.317992887065806	39.0	35.0	40.0	33.0	41.0
21	37.17465417175772	39.0	35.0	40.0	33.0	41.0
22	37.22151008029324	39.0	35.0	40.0	33.0	41.0
23	37.15512087624367	39.0	35.0	40.0	33.0	41.0
24	37.06645466049921	38.0	35.0	40.0	33.0	41.0
25	36.98273040670274	38.0	35.0	40.0	33.0	41.0
26	36.930201605864895	38.0	35.0	40.0	33.0	41.0
27	36.84598097399197	38.0	35.0	40.0	33.0	41.0
28	36.703433190783734	38.0	35.0	40.0	32.0	41.0
29	36.634278451736776	38.0	35.0	40.0	32.0	41.0
30	36.45564780066329	38.0	35.0	40.0	31.0	41.0
31	36.16658666433933	38.0	35.0	40.0	31.0	41.0
32	35.76638593122709	38.0	35.0	40.0	30.0	41.0
33	35.324413073834876	38.0	35.0	40.0	25.0	41.0
34	35.011514880432884	38.0	35.0	40.0	23.0	41.0
35	34.673857784953746	38.0	35.0	40.0	21.0	41.0
36	34.37237083260604	38.0	34.0	40.0	20.0	41.0
37	34.20209133356607	38.0	34.0	40.0	18.0	41.0
38	34.07411306510735	38.0	33.0	40.0	18.0	41.0
39	33.946314801885144	38.0	33.0	40.0	18.0	41.0
40	33.75267280502705	38.0	33.0	40.0	16.0	41.0
41	33.692507418397625	38.0	33.0	40.0	16.0	41.0
42	33.66379385582126	37.0	33.0	40.0	15.0	41.0
43	33.55309063536394	37.0	33.0	40.0	15.0	41.0
44	33.38163292023041	37.0	33.0	40.0	15.0	41.0
45	33.25106366730669	37.0	33.0	40.0	15.0	41.0
46	32.93711817071042	37.0	32.0	40.0	13.0	41.0
47	32.7586457060569	36.0	31.0	40.0	12.0	41.0
48	32.756005629254666	36.0	31.0	40.0	12.0	41.0
49	32.84702827718625	36.0	32.0	40.0	12.0	41.0
50	32.78252094606388	36.0	32.0	40.0	12.0	41.0
51	31.75314736428696	35.0	30.0	39.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	1.0
12	2.0
13	1.0
14	4.0
15	11.0
16	11.0
17	23.0
18	47.0
19	107.0
20	182.0
21	352.0
22	548.0
23	856.0
24	1617.0
25	2713.0
26	4386.0
27	5028.0
28	4679.0
29	4493.0
30	4586.0
31	4998.0
32	5824.0
33	7206.0
34	10590.0
35	15237.0
36	20203.0
37	24332.0
38	37779.0
39	27504.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.08812620003491	4.717773607959504	41.67339413510211	30.520706056903474
2	35.29793593995462	4.91196107523128	40.670274044335834	19.119828940478268
3	18.958915168441266	4.883596613719672	57.566765578635014	18.59072263920405
4	14.303870658055507	5.256698376680049	58.059870832606045	22.379560132658405
5	13.299114156048175	5.889989526968057	58.82025658928259	21.99063972770117
6	16.97776662593821	7.0714784430092505	61.59506458369698	14.35569034735556
7	64.7047914121138	2.3782510036655613	27.640076802234248	5.276880781986385
8	63.29038662942922	3.045361319602025	27.092969977308428	6.571282073660324
9	57.64476784779193	6.0012654913597485	29.65177168790365	6.702194972944668
10	23.761782160935592	27.100061092686335	34.40609181358003	14.732064932798044
11	19.603661197416653	18.365443358352245	40.82846046430441	21.202434979926686
12	16.60848315587363	17.356323093035435	45.57841682667132	20.45677692441962
13	18.786001047303195	18.41671757723861	47.63647669750392	15.160804677954268
14	16.953765927736082	20.014400418921277	45.780240879734684	17.25159277360796
15	12.9331035084657	18.34307907139117	47.57483854075755	21.14897887938558
16	13.34438820038401	19.693118345260956	44.32165299354163	22.640840460813404
17	13.050379647407926	19.29601588409845	43.680725257462036	23.972879211031596
18	15.20007854773957	16.86212689823704	46.7997250829115	21.138069471111887
19	14.642062314540057	19.696936638156746	46.21170797695933	19.449293070343863
20	15.616817943794729	22.470108221330076	43.87982195845697	18.033251876418223
21	14.190412812009075	24.72617385233025	43.80291063012742	17.28050270553325
22	13.007832955140513	21.85209024262524	42.104861232326755	23.03521556990749
23	12.657095479141212	23.3712253447373	40.648455227788446	23.32322394833304
24	13.264749519986035	22.126461860708673	41.34829376854599	23.260494850759294
25	13.628032815500088	24.68417263047652	38.22602112061442	23.461773433408972
26	14.163684761738523	24.434892651422587	40.31626374585443	21.085158840984462
27	15.363174201431315	22.676296037702915	41.82121661721068	20.139313143655087
28	11.818161982894047	21.974275615290626	41.74812358177692	24.4594388200384
29	15.357174026880783	19.76730232152208	39.75224733810438	25.123276313492752
30	19.31838017105952	21.529171757723862	39.357326758596614	19.795121312620005
31	18.41671757723861	26.171124978181183	33.02986995985337	22.382287484726827
32	16.107741316111014	24.725628381916565	32.87331995112585	26.29331035084657
33	16.460660673765055	22.3833784255542	33.766255018327804	27.389705882352942
34	17.29741228835748	21.565172805027053	33.30642345959155	27.830991447023912
35	19.234923197765752	21.79590679001571	32.3633051143306	26.605864897887937
36	19.23001396404259	24.20143131436551	34.86865072438471	21.699903997207194
37	15.80718711817071	23.20976610228661	34.05808168964916	26.924965089893526
38	14.87934194449293	24.237432361668702	30.611799615988826	30.27142607784954
39	17.672695932972594	25.45328591377204	30.295426776051666	26.578591377203704
40	20.114766975039274	27.560983592249954	30.477068423808696	21.847181008902076
41	17.61814889160412	25.085638854948506	30.552888811310876	26.743323442136496
42	19.04891778669925	20.235315936463607	36.19632571129342	24.519440565543725
43	18.02016058648979	18.44835486123233	38.5920317681969	24.93945278408099
44	18.841093559085355	20.079311398149766	33.4978835747949	27.581711467969978
45	21.524262524000697	20.034582824227616	31.16872490836097	27.27242974341072
46	21.302256065631	21.64372054459766	33.910259207540584	23.143764182230754
47	15.248625414557516	24.38361843253622	38.03947023913423	22.328285913772035
48	15.13134927561529	21.84609006807471	39.49423983243149	23.528320823878513
49	20.06622010822133	18.43471810089021	39.171866817943794	22.327194972944667
50	18.8465482632222	17.618694362017802	35.95631872927213	27.578438645487868
51	17.729970326409493	16.4017498690871	33.25896753360097	32.60931227090242
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	511.0
1	712.0
2	913.0
3	7091.0
4	13269.0
5	7979.0
6	2689.0
7	2653.5
8	2618.0
9	2556.0
10	2494.0
11	2413.0
12	2332.0
13	2190.0
14	2048.0
15	1879.5
16	1711.0
17	1640.5
18	1570.0
19	1397.0
20	1224.0
21	1163.0
22	1102.0
23	1053.5
24	1005.0
25	1046.5
26	1146.5
27	1205.0
28	1273.0
29	1341.0
30	1461.5
31	1582.0
32	1703.0
33	1824.0
34	2038.0
35	2252.0
36	2509.5
37	2767.0
38	3121.0
39	3475.0
40	4401.0
41	5327.0
42	6564.5
43	7802.0
44	9410.5
45	11019.0
46	14229.0
47	17439.0
48	21113.0
49	24787.0
50	23876.5
51	22966.0
52	19135.0
53	15304.0
54	12183.0
55	9062.0
56	7348.0
57	5634.0
58	4711.5
59	3789.0
60	3404.0
61	3019.0
62	2741.0
63	2463.0
64	2249.5
65	2036.0
66	1663.5
67	1291.0
68	1030.5
69	770.0
70	669.5
71	569.0
72	485.0
73	401.0
74	350.5
75	245.0
76	190.0
77	138.0
78	86.0
79	60.5
80	35.0
81	23.5
82	12.0
83	8.0
84	4.0
85	3.0
86	2.0
87	1.0
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	183328.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.49155611799616
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.79804211492377	41.08046779542678
2	11.475042063937185	12.276357130389249
3	4.889614031509713	7.846591900855297
4	2.5105797175342883	5.371792633967534
5	1.3480854535257227	3.605559434456275
6	0.7902921531637179	2.5364374236341423
7	0.44766226482435123	1.6762305812532727
8	0.317136593076021	1.3571303892476871
9	0.21516341202263806	1.0358483155873626
>10	1.1033498189976036	10.545034037353814
>50	0.07138122673736806	2.639531331820562
>100	0.026513027073879567	2.2380651073485773
>500	0.005098659052669148	1.9686027229883052
>1k	0.0010197318105338296	0.6992930703438646
>5k	0.0010197318105338296	5.123058125327282
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9392	5.123058125327282	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCC	1282	0.6992930703438646	No Hit
CTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTCTGCT	891	0.4860141385931227	No Hit
CCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTCTGC	710	0.38728399371618083	No Hit
TCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTCTGC	680	0.370919881305638	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGC	676	0.36873799965089893	No Hit
GCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTCTGC	652	0.35564670972246465	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	334	0.18218711817071043	No Hit
TCCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTCTG	329	0.17945976610228662	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	269	0.14673154128120092	No Hit
GCCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTCTG	252	0.13745854424855997	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCG	252	0.13745854424855997	No Hit
TTCCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTCT	210	0.11454878687379996	No Hit
TGCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTCTG	195	0.10636673066852853	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5329245941700123	0.0
2	0.0	0.0	0.0	1.817507418397626	0.0
3	0.0	0.0	0.0	2.676623319951126	0.0
4	0.0	0.0	0.0	3.736472333740618	0.0
5	0.0	0.0	0.0	5.880716529935416	0.0
6	0.0	0.0	0.0	7.65895007854774	0.0
7	5.454704136847617E-4	0.0	0.0	9.430637982195845	0.0
8	5.454704136847617E-4	0.0	0.0	12.017258683888986	0.0
9	5.454704136847617E-4	0.0	0.0	13.282750043637632	0.0
10	5.454704136847617E-4	0.0	0.0	16.00628381916565	0.0
11	5.454704136847617E-4	0.0	0.0	21.654629952871357	0.0
12	5.454704136847617E-4	0.0	0.0	26.607501309128992	0.0
13	5.454704136847617E-4	0.0	0.0	28.44464566241927	0.0
14	0.0010909408273695235	0.0	0.0	29.0735730493978	0.002727352068423809
15	0.0010909408273695235	0.0	0.0	30.03796474079246	0.002727352068423809
16	0.0010909408273695235	0.0	0.0	32.14238959678827	0.002727352068423809
17	0.0010909408273695235	0.0	0.0	34.935743585267936	0.002727352068423809
18	0.0010909408273695235	0.0	0.0	37.333086053412465	0.002727352068423809
19	0.0010909408273695235	0.0	0.0	38.68858003141909	0.002727352068423809
20	0.0010909408273695235	0.0	0.0	40.08007505672892	0.002727352068423809
21	0.0010909408273695235	0.0	0.0	41.59648280677256	0.002727352068423809
22	0.0010909408273695235	0.0	0.0	42.88870221679176	0.002727352068423809
23	0.0010909408273695235	0.0	0.0	43.8285477395706	0.002727352068423809
24	0.0010909408273695235	0.0	0.0	44.34674463257113	0.002727352068423809
25	0.0010909408273695235	0.0	0.0	44.74111974166521	0.002727352068423809
26	0.0010909408273695235	0.0	0.0	45.01821871181707	0.002727352068423809
27	0.0010909408273695235	0.0	0.0	45.25168004887415	0.002727352068423809
28	0.0010909408273695235	0.0	0.0	45.46932274393437	0.002727352068423809
29	0.0010909408273695235	0.0	0.0	45.689147320649326	0.002727352068423809
30	0.0010909408273695235	0.0	0.0	45.97006458369698	0.002727352068423809
31	0.0010909408273695235	0.0	0.0	46.14788793855821	0.002727352068423809
32	0.0010909408273695235	0.0	0.0	46.410259207540584	0.002727352068423809
33	0.0010909408273695235	0.0	0.0	46.60117385233025	0.002727352068423809
34	0.0010909408273695235	0.0	0.0	46.764269506021996	0.002727352068423809
35	0.0010909408273695235	0.0	0.0	46.92900157095479	0.002727352068423809
36	0.0010909408273695235	0.0	0.0	47.063187292721246	0.002727352068423809
37	0.0010909408273695235	0.0	0.0	47.20719148193402	0.002727352068423809
38	0.0010909408273695235	0.0	0.0	47.38719671844999	0.002727352068423809
39	0.0010909408273695235	0.0	0.0	47.621203525920755	0.002727352068423809
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAT	20	7.017761E-4	45.000004	6
ATGGGAT	20	7.017761E-4	45.000004	5
TTGGCGA	20	7.017761E-4	45.000004	5
CCTAGCA	20	7.017761E-4	45.000004	45
ACCATAG	20	7.017761E-4	45.000004	23
GCGCGAC	20	7.017761E-4	45.000004	9
CGACCTC	20	7.017761E-4	45.000004	12
GATTTCA	20	7.017761E-4	45.000004	9
TAACGTC	20	7.017761E-4	45.000004	32
TACGGGG	20	7.017761E-4	45.000004	4
CGCGACC	20	7.017761E-4	45.000004	10
AGCGCTT	20	7.017761E-4	45.000004	37
GCCTAGC	20	7.017761E-4	45.000004	44
TATAGGG	20	7.017761E-4	45.000004	3
CGATGTT	20	7.017761E-4	45.000004	10
TTGCCTA	20	7.017761E-4	45.000004	42
TGGGCGC	40	6.7611836E-9	45.000004	6
GGCGCGA	20	7.017761E-4	45.000004	8
TGTACGG	20	7.017761E-4	45.000004	2
AGGGCGA	20	7.017761E-4	45.000004	6
>>END_MODULE