##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933733.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	184554
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.927300410719898	33.0	31.0	34.0	30.0	34.0
2	32.133185950995376	34.0	31.0	34.0	30.0	34.0
3	32.25750728783987	33.0	31.0	34.0	30.0	34.0
4	35.80545531389187	37.0	35.0	37.0	35.0	37.0
5	35.896588532353675	37.0	35.0	37.0	35.0	37.0
6	35.88423984308116	37.0	35.0	37.0	35.0	37.0
7	35.87994841618171	37.0	35.0	37.0	35.0	37.0
8	35.737188031687204	37.0	35.0	37.0	35.0	37.0
9	37.54247537306154	39.0	37.0	39.0	35.0	39.0
10	37.0968551209944	39.0	37.0	39.0	33.0	39.0
11	37.1583926655613	39.0	37.0	39.0	34.0	39.0
12	37.15161416170877	39.0	37.0	39.0	34.0	39.0
13	37.117250235703374	39.0	37.0	39.0	33.0	39.0
14	38.23830423615852	40.0	38.0	41.0	33.0	41.0
15	38.24650237870759	40.0	38.0	41.0	33.0	41.0
16	38.377298785179406	40.0	38.0	41.0	34.0	41.0
17	38.37666482438744	40.0	38.0	41.0	34.0	41.0
18	38.18502985576038	39.0	38.0	40.0	34.0	41.0
19	37.924136025228385	39.0	37.0	41.0	34.0	41.0
20	37.49355202271422	39.0	35.0	40.0	34.0	41.0
21	37.384445744876835	39.0	35.0	40.0	33.0	41.0
22	37.385697411055844	39.0	35.0	40.0	33.0	41.0
23	37.286571951840656	39.0	35.0	40.0	33.0	41.0
24	37.19078968757112	39.0	35.0	40.0	33.0	41.0
25	37.11346814482482	39.0	35.0	40.0	33.0	41.0
26	37.11023873771362	39.0	35.0	40.0	33.0	41.0
27	37.04392752256792	38.0	35.0	40.0	33.0	41.0
28	36.98402635542985	38.0	35.0	40.0	33.0	41.0
29	36.82453374080215	38.0	35.0	40.0	33.0	41.0
30	36.54945436024145	38.0	35.0	40.0	32.0	41.0
31	36.27917032413277	38.0	35.0	40.0	31.0	41.0
32	35.99232744887675	38.0	35.0	40.0	30.0	41.0
33	35.48638880761187	38.0	35.0	40.0	26.0	41.0
34	35.15986648894091	38.0	35.0	40.0	24.0	41.0
35	34.89964454847904	38.0	35.0	40.0	22.0	41.0
36	34.59532711293171	38.0	35.0	40.0	21.0	41.0
37	34.451867746025556	38.0	34.0	40.0	20.0	41.0
38	34.31535485548945	38.0	34.0	40.0	18.0	41.0
39	34.23458174843135	38.0	34.0	40.0	18.0	41.0
40	34.07647084322204	38.0	34.0	40.0	18.0	41.0
41	34.01820605351279	38.0	34.0	40.0	18.0	41.0
42	33.883616719225806	38.0	33.0	40.0	16.0	41.0
43	33.75723636442451	38.0	33.0	40.0	15.0	41.0
44	33.60289671315712	38.0	33.0	40.0	15.0	41.0
45	33.45549270132319	37.0	33.0	40.0	15.0	41.0
46	33.14706264833057	37.0	33.0	40.0	13.0	41.0
47	33.08476651820064	37.0	32.0	40.0	13.0	41.0
48	33.03467277869892	37.0	32.0	40.0	13.0	41.0
49	33.101206151045226	37.0	32.0	40.0	13.0	41.0
50	32.93889593289769	36.0	32.0	40.0	12.0	41.0
51	32.073003023505315	35.0	31.0	39.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	2.0
10	3.0
11	5.0
12	4.0
13	7.0
14	4.0
15	10.0
16	12.0
17	22.0
18	58.0
19	101.0
20	226.0
21	314.0
22	529.0
23	862.0
24	1545.0
25	2802.0
26	4254.0
27	4863.0
28	4582.0
29	4424.0
30	4450.0
31	4844.0
32	5451.0
33	7050.0
34	10030.0
35	14561.0
36	19534.0
37	23239.0
38	38093.0
39	32655.0
40	16.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.33300822523489	4.958982194913142	41.17548251460278	30.532527065249194
2	35.36742633592336	4.878788863963935	40.653684016602185	19.100100783510516
3	19.149950691938404	4.965484356881997	57.28675617976311	18.597808771416496
4	14.553464026788907	5.36970209261246	57.73377981512186	22.34305406547677
5	13.411792754424178	5.931055409256912	58.718857353403344	21.93829448291557
6	16.957638414772912	7.054845736207289	61.36523727472718	14.622278574292618
7	64.74744519219307	2.4448129002893464	27.61305634123346	5.1946855662841225
8	63.38795149387171	3.045721035577663	26.851761544046727	6.714565926503896
9	57.47098410221398	6.070310044756548	29.712170963512037	6.746534889517432
10	23.790326950377665	27.06091442071155	34.3682607800427	14.780497848868082
11	19.83051031134519	18.298709320849184	40.923523738309655	20.947256629495975
12	16.744692610292923	17.57859488279853	45.17106104446395	20.505651462444597
13	18.919665788874802	18.65090976082881	47.391007509997074	15.038416940299316
14	17.164623904114784	19.909078101802184	45.516217475643984	17.41008051843905
15	13.036834747553561	18.588055528463215	47.341157601569186	21.033952122414036
16	13.463268203344278	19.74814959307303	44.18815089350542	22.60043131007727
17	13.030874432415446	19.651159010370947	43.55689933569579	23.76106722151782
18	15.264908915547753	16.725727971217097	46.72832883600464	21.28103427723051
19	14.669961095397552	19.860311887035774	45.925311832851094	19.544415184715586
20	15.801879124808998	22.345221452799723	43.8402852281717	18.01261419421958
21	14.572970512695472	24.891901557267794	43.44798812271747	17.087139807319268
22	13.2476131647106	21.6326928703794	42.17031329583753	22.949380669072468
23	12.940927858512955	23.575755605405462	40.218039164688925	23.265277371392653
24	13.468686671651659	22.185376637732045	41.32232300573274	23.023613684883557
25	13.69897157471526	24.829047324902195	38.09399958819641	23.377981512186135
26	14.320469889571616	24.143611084018772	40.2976906488074	21.23822837760222
27	15.490859043965452	22.658408920966224	41.671814211558676	20.17891782350965
28	12.063135992717578	21.937210789254095	41.69294623795746	24.306706980070874
29	15.536916024578174	20.001192063027624	39.57378328294158	24.888108629452628
30	19.29462379574542	21.570380484844545	39.430735719626774	19.70425999978326
31	18.55825395277263	26.20262904082274	32.81261852899423	22.426498477410405
32	16.272202173889486	24.791659893581283	32.74976429662863	26.186373635900605
33	16.435839916772327	22.55599986995676	33.69366147577403	27.314498737496884
34	17.39436696034765	21.969721599098367	33.269937254137	27.365974186416985
35	19.113105107448227	21.775198586863464	32.210084853213694	26.901611452474615
36	19.374817126694627	24.131690453742536	34.790359461187506	21.703132958375328
37	15.916750652925431	23.062084809865947	34.32003641210702	26.701128125101597
38	15.039500633960792	24.421578508187306	30.47238206703729	30.06653879081461
39	17.534163442678025	25.472761359818808	30.533610758910672	26.4594644385925
40	20.27645025304247	27.381145897677644	30.500016255404923	21.842387593874964
41	17.456137499051767	25.059874074796536	30.655526295826697	26.828462130324997
42	19.272408075685167	20.26182038861255	35.81607551177433	24.649696023927955
43	18.206053512793	18.61460602316937	38.526393359125244	24.652947104912386
44	18.835679530110426	20.230935119260486	33.56795301104284	27.365432339586242
45	21.454425263066636	20.00986161231943	31.266187674068295	27.26952545054564
46	21.441962785959664	21.674956923176953	34.07728903193645	22.805791258926927
47	15.250279051117829	24.45138008387789	38.128677785363635	22.169663079640646
48	15.226979637396102	22.245521635943952	39.08937221626191	23.438126510398043
49	20.0380376475178	18.57125827671034	39.055777712756154	22.334926363015704
50	19.15862024123021	17.739523391527683	35.920651950106745	27.181204417135362
51	17.71188920316005	16.600019506485904	33.18486730171115	32.503223988642894
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	441.0
1	694.5
2	948.0
3	7051.5
4	13155.0
5	7930.5
6	2706.0
7	2662.0
8	2618.0
9	2580.5
10	2543.0
11	2437.0
12	2331.0
13	2207.5
14	2084.0
15	1918.5
16	1753.0
17	1684.0
18	1615.0
19	1439.5
20	1264.0
21	1166.0
22	1068.0
23	1050.0
24	1032.0
25	1057.0
26	1170.5
27	1259.0
28	1349.5
29	1440.0
30	1473.5
31	1507.0
32	1705.0
33	1903.0
34	2108.0
35	2313.0
36	2565.5
37	2818.0
38	3207.0
39	3596.0
40	4497.0
41	5398.0
42	6676.5
43	7955.0
44	9454.5
45	10954.0
46	14155.0
47	17356.0
48	20935.5
49	24515.0
50	24017.0
51	23519.0
52	19499.0
53	15479.0
54	12286.0
55	9093.0
56	7327.5
57	5562.0
58	4699.5
59	3837.0
60	3446.0
61	3055.0
62	2787.5
63	2520.0
64	2268.5
65	2017.0
66	1684.0
67	1351.0
68	1111.0
69	871.0
70	709.5
71	548.0
72	457.0
73	366.0
74	343.5
75	260.5
76	200.0
77	146.0
78	92.0
79	67.5
80	43.0
81	32.5
82	22.0
83	11.0
84	0.0
85	0.0
86	0.0
87	1.0
88	2.0
89	2.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	184554.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.664510116280326
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.85985460420032	41.24646444942944
2	11.35702746365105	12.189386304279507
3	4.945476575121163	7.961897330862512
4	2.501009693053312	5.368618398950985
5	1.3428917609046849	3.603281424406948
6	0.7895799676898223	2.5423453298221657
7	0.46445880452342486	1.7447467949759963
8	0.3079563812600969	1.3221062670004442
9	0.21102584814216477	1.0192138886179656
>10	1.1147011308562196	10.37257388081537
>50	0.07269789983844911	2.6740141096914725
>100	0.026252019386106624	2.2091095289183653
>500	0.005048465266558966	2.062269037788398
>1k	0.0010096930533117932	0.7081938077744183
>5k	0.0010096930533117932	4.975779446666016
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9183	4.975779446666016	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCC	1307	0.7081938077744183	No Hit
CTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTCTGCT	892	0.4833273730181952	No Hit
CCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTCTGC	828	0.4486491758509704	No Hit
TCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTCTGC	735	0.3982574205923469	No Hit
GCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTCTGC	687	0.37224877271692836	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGC	664	0.35978629560995695	No Hit
TCCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTCTG	269	0.1457567974684916	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	266	0.14413125697627793	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	258	0.13979648233037487	No Hit
GCCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTCTG	238	0.1289595457156171	No Hit
TTCCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTCT	228	0.12354107740823823	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCG	226	0.12245738374676246	No Hit
CGCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTCTG	197	0.10674382565536375	No Hit
TGCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTCTG	190	0.10295089784019852	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5152963360317305	0.0
2	0.0	0.0	0.0	1.880208502660468	0.0
3	0.0	0.0	0.0	2.708150460027959	0.0
4	0.0	0.0	0.0	3.7988881303033257	0.0
5	0.0	0.0	0.0	5.955980363470854	0.0
6	0.0	0.0	0.0	7.728903193645221	0.0
7	0.0	0.0	0.0	9.378826793242085	0.0
8	0.0	0.0	0.0	11.965603563184759	0.0
9	0.0	0.0	0.0	13.20914203972821	0.0
10	0.0	0.0	0.0	15.918376193417645	0.0
11	0.0	0.0	0.0	21.41270305709982	0.0
12	0.0	0.0	0.0	26.32617011823098	0.0
13	0.0	0.0	0.0	28.097467407913133	0.0
14	0.0	0.0	0.0	28.73955590233753	0.001083693661475774
15	0.0	0.0	0.0	29.675325379021857	0.001083693661475774
16	0.0	0.0	0.0	31.71537869675	0.001083693661475774
17	0.0	0.0	0.0	34.36663523955048	0.001083693661475774
18	0.0	0.0	0.0	36.761598231411945	0.001083693661475774
19	0.0	0.0	0.0	38.14276580296282	0.001083693661475774
20	0.0	0.0	0.0	39.47299977242433	0.001083693661475774
21	0.0	0.0	0.0	40.94032099006253	0.001083693661475774
22	0.0	0.0	0.0	42.14972311626949	0.001083693661475774
23	0.0	0.0	0.0	43.08549259295383	0.001083693661475774
24	0.0	0.0	0.0	43.57532212794087	0.001083693661475774
25	0.0	0.0	0.0	43.92156225278239	0.001083693661475774
26	0.0	0.0	0.0	44.19194382132059	0.001083693661475774
27	0.0	0.0	0.0	44.44010966979854	0.001083693661475774
28	0.0	0.0	0.0	44.64655331230968	0.001083693661475774
29	0.0	0.0	0.0	44.8399926308831	0.001083693661475774
30	0.0	0.0	0.0	45.11904374871311	0.001083693661475774
31	0.0	0.0	0.0	45.28647441941112	0.001083693661475774
32	0.0	0.0	0.0	45.515133781982506	0.001083693661475774
33	0.0	0.0	0.0	45.6966524702797	0.001083693661475774
34	0.0	0.0	0.0	45.86245760048549	0.001083693661475774
35	0.0	0.0	0.0	46.042350748290474	0.001083693661475774
36	0.0	0.0	0.0	46.18810754575897	0.001083693661475774
37	0.0	0.0	0.0	46.33928281153484	0.001083693661475774
38	0.0	0.0	0.0	46.50996456321727	0.001083693661475774
39	0.0	0.0	0.0	46.73591469163497	0.001083693661475774
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTGTTG	30	2.1555734E-6	45.000004	1
GTTTCGG	35	1.2045348E-7	45.000004	2
CGCGGGA	70	0.0	45.000004	4
GCTTGCG	35	1.2045348E-7	45.000004	1
ATTGGGA	70	0.0	45.000004	4
GTCCAGG	30	2.1555734E-6	45.000004	2
TGTTTCG	25	3.8780465E-5	45.0	1
ATGGGCG	25	3.8780465E-5	45.0	5
ATGGGCA	20	7.0178864E-4	45.0	5
TCGTTAG	20	7.0178864E-4	45.0	1
GGATGAT	20	7.0178864E-4	45.0	8
CCTAGGG	45	3.8016879E-10	45.0	3
TTGCTCG	20	7.0178864E-4	45.0	1
TGGGTAC	45	3.8016879E-10	45.0	6
GGCCGAT	20	7.0178864E-4	45.0	8
GGATAGT	25	3.8780465E-5	45.0	8
TCGGAAT	20	7.0178864E-4	45.0	5
CGAGGGA	20	7.0178864E-4	45.0	4
CGGGATA	25	3.8780465E-5	45.0	6
GGACGGA	20	7.0178864E-4	45.0	8
>>END_MODULE