FastQCFastQC Report
Sat 14 Jan 2017
SRR2933732.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933732.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1371056
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT690055.0329818767431815No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCC19450.14186145569546393No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA15120.11027995938896734No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCGG302.1658088E-644.9999962
GCTATCG302.1658088E-644.9999961
CGTTTTT385350.044.4803431
AGTGCGC1000.040.513
GTTTTTT434450.039.898722
TCGTTCA403.458881E-739.37516
CGATATG403.458881E-739.37510
CAACGAG1750.038.57142614
GAGCGAC1750.038.57142618
GGCAACG1000.038.258
CTTCGCG900.037.51
TTGCGGA900.037.53
TTCTTCG2300.037.1739121
TGGGCGA23500.037.1489376
CGTTTGG6850.037.1167872
CGAATAT1100.036.8181814
TTGGGCG17200.036.7587245
CACAACG1900.036.71052612
TCCGATA250.0021072536.014
ACTTGCG250.0021072536.01
Produced by FastQC (version 0.11.5)