FastQCFastQC Report
Sat 14 Jan 2017
SRR2933731.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933731.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1392427
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT685344.921909730276703No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCC19730.14169504038631828No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA15750.11311185433778574No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA14030.10075932167359582No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGAC406.8175723E-945.09
GGCGCGA406.8175723E-945.08
CGTTTTT385650.044.4224051
TCTTGCG2450.040.4081651
GGCAACG1850.040.1351368
CGCGACC451.9288564E-840.010
GTTTTTT440950.039.489172
TAACGTC403.4589175E-739.37532
CTCGTAG301.14004586E-437.5000041
TCGTAGG601.5643309E-1037.5000042
GTTTGCG1800.037.51
TGGGAAC20450.036.638146
CGTTTGG7550.036.3576162
TCAAGCG6650.036.20300717
GCGTTTG3000.036.01
TGCGCGC1500.036.015
TACGAAT1250.036.012
TCGACGG250.002107261735.9999962
TTGGGAT54700.035.9506385
GGGCCGA4700.035.9042557
Produced by FastQC (version 0.11.5)