SRR2933730.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933730.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	265635
Sequences flagged as poor quality	0
Sequence length	51
%GC	32

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	36236	13.64127468142376	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCC	5529	2.081427522728556	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGC	3100	1.1670148888512433	No Hit
CTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGCT	1937	0.7291960773241478	Illumina PCR Primer Index 7 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGC	1834	0.6904210665010259	Illumina PCR Primer Index 7 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGC	1353	0.5093455305212039	RNA PCR Primer, Index 14 (95% over 24bp)
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCG	1083	0.40770229826641824	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	872	0.32826999454138195	No Hit
GCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGC	807	0.3038003275170817	RNA PCR Primer, Index 14 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTG	561	0.2111920492404992	Illumina PCR Primer Index 7 (95% over 21bp)
CGTTTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATG	542	0.20403937734108832	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	475	0.17881679748527113	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTC	472	0.17768742823799574	No Hit
TTCCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCT	465	0.1750522333276865	RNA PCR Primer, Index 14 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTG	458	0.17241703841737724	Illumina PCR Primer Index 7 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	389	0.1464415457300431	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	374	0.14079469949366613	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTC	365	0.13740659175183992	RNA PCR Primer, Index 14 (95% over 21bp)
CGTTTTTTCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGT	346	0.1302539198524291	No Hit
TGCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTG	334	0.1257364428633275	RNA PCR Primer, Index 14 (95% over 23bp)
TTCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTG	330	0.12423061720029363	RNA PCR Primer, Index 14 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	317	0.11933668379543359	No Hit
TTTCCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTC	311	0.1170779453008828	RNA PCR Primer, Index 14 (95% over 21bp)
CGTCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCT	277	0.10427842716509494	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGGTA	20	7.023481E-4	45.000004	5
GGATGCC	20	7.023481E-4	45.000004	8
TTTGTCG	20	7.023481E-4	45.000004	1
GTTTAGG	40	6.7811925E-9	45.000004	2
TCCCTTG	35	1.2072087E-7	45.000004	1
TTCACGG	20	7.023481E-4	45.000004	2
CTTATTG	20	7.023481E-4	45.000004	2
CGGGCGA	20	7.023481E-4	45.000004	6
ACTAATC	20	7.023481E-4	45.000004	10
CCCTTGG	35	1.2072087E-7	45.000004	2
GCTAGCT	20	7.023481E-4	45.000004	4
ATTGGGC	35	1.2072087E-7	45.000004	4
ATTGGAT	20	7.023481E-4	45.000004	11
TGCTAGC	20	7.023481E-4	45.000004	3
GGCGATT	75	0.0	45.000004	8
GGGATCG	20	7.023481E-4	45.000004	8
GCTTTGT	20	7.023481E-4	45.000004	2
TGCCGGG	20	7.023481E-4	45.000004	3
CTTGTTG	45	3.8198777E-10	45.0	1
ATGGGAC	25	3.882681E-5	45.0	5

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content