##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933730.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	265635
Sequences flagged as poor quality	0
Sequence length	51
%GC	32
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.958593558830728	33.0	31.0	34.0	30.0	34.0
2	32.22306548459352	34.0	31.0	34.0	30.0	34.0
3	32.18924464020178	33.0	31.0	34.0	30.0	34.0
4	35.9022192105709	37.0	35.0	37.0	35.0	37.0
5	35.952494964895436	37.0	35.0	37.0	35.0	37.0
6	35.940892578161765	37.0	35.0	37.0	35.0	37.0
7	35.96515142959324	37.0	35.0	37.0	35.0	37.0
8	35.778353756093885	37.0	35.0	37.0	35.0	37.0
9	37.60697950194816	39.0	37.0	39.0	35.0	39.0
10	37.22834716810661	39.0	37.0	39.0	34.0	39.0
11	37.279018201667704	39.0	37.0	39.0	34.0	39.0
12	37.349027801306306	39.0	37.0	39.0	34.0	39.0
13	37.29597379863346	39.0	37.0	39.0	34.0	39.0
14	38.362456001656405	40.0	38.0	41.0	33.0	41.0
15	38.38487021665067	40.0	38.0	41.0	33.0	41.0
16	38.446499896474485	40.0	38.0	41.0	34.0	41.0
17	38.43974626837578	40.0	38.0	41.0	33.0	41.0
18	37.86562388239501	39.0	38.0	40.0	34.0	41.0
19	37.22164247934195	37.0	37.0	40.0	34.0	41.0
20	36.40079055847309	36.0	35.0	40.0	33.0	41.0
21	36.26003726918516	35.0	35.0	40.0	33.0	41.0
22	36.255956481638336	35.0	35.0	40.0	33.0	41.0
23	36.198125247049525	35.0	35.0	40.0	33.0	41.0
24	36.010947352570255	35.0	35.0	40.0	32.0	41.0
25	35.88903194232688	35.0	35.0	40.0	32.0	41.0
26	35.85232744179043	35.0	35.0	40.0	32.0	41.0
27	35.80479605473676	35.0	35.0	40.0	32.0	41.0
28	35.69164078528809	35.0	35.0	40.0	31.0	41.0
29	35.58709883863196	35.0	35.0	40.0	31.0	41.0
30	35.34936661208049	35.0	35.0	40.0	30.0	41.0
31	34.792647806200236	35.0	35.0	40.0	27.0	41.0
32	34.14583545090067	35.0	34.0	40.0	22.0	41.0
33	33.39606226589117	35.0	33.0	40.0	18.0	41.0
34	32.710264084175655	35.0	33.0	40.0	12.0	41.0
35	32.19949931296704	35.0	32.0	40.0	10.0	41.0
36	31.878419635966647	35.0	31.0	40.0	9.0	41.0
37	31.62583996837766	35.0	31.0	40.0	8.0	41.0
38	31.51750334105069	35.0	30.0	40.0	8.0	41.0
39	31.396355901895458	35.0	30.0	40.0	8.0	41.0
40	31.13123647109756	35.0	29.0	40.0	8.0	41.0
41	30.900272930901426	35.0	27.0	40.0	7.0	41.0
42	30.717537222128108	35.0	26.0	39.0	7.0	41.0
43	30.536442863327498	35.0	25.0	39.0	7.0	41.0
44	30.382532422308806	35.0	24.0	39.0	7.0	41.0
45	30.368257195023247	35.0	23.0	39.0	7.0	41.0
46	29.981899975530332	35.0	23.0	39.0	7.0	41.0
47	29.717367816741017	35.0	23.0	39.0	7.0	40.0
48	29.65523368532008	35.0	23.0	39.0	7.0	40.0
49	29.667084533288158	35.0	23.0	39.0	7.0	40.0
50	29.52610913471493	35.0	22.0	39.0	7.0	40.0
51	28.534530464735447	35.0	20.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	7.0
13	5.0
14	18.0
15	7.0
16	30.0
17	66.0
18	163.0
19	389.0
20	666.0
21	1013.0
22	1652.0
23	2493.0
24	4267.0
25	7766.0
26	11334.0
27	11504.0
28	10012.0
29	8627.0
30	8514.0
31	8631.0
32	9516.0
33	10859.0
34	14369.0
35	24718.0
36	33479.0
37	27989.0
38	42949.0
39	24585.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	14.883580853426695	3.447964311931786	31.723982155965892	49.94447267867563
2	53.0046868823762	3.81990325070115	29.866546200613627	13.308863666309033
3	11.824872475389162	4.1146686242400285	70.12630112748697	13.934157772883845
4	10.427842716509495	3.605323093718825	70.77907655241215	15.187757637359534
5	8.914111468744707	4.412069192689216	70.40826698289005	16.26555235567602
6	11.660361021702712	5.265872343629416	73.22830199333671	9.84546464133115
7	42.35209968565889	2.5828674685188324	49.83040638470081	5.234626461121464
8	40.324505430383795	3.043273664991436	49.49159561051819	7.140625294106575
9	36.37321889058294	4.591262446590246	52.19907015265308	6.836448510173734
10	17.972029288309148	15.805899072034935	55.16667607807706	11.055395561578859
11	11.338867242644984	13.27046511190167	57.53722212810813	17.85344551734523
12	10.447794906544695	11.47702674722834	62.30880719784667	15.766371148380296
13	14.763867713215504	12.254785702185329	62.26664407928172	10.714702505317447
14	11.981101887928924	14.578651156662337	60.49428727389087	12.945959681517872
15	7.550586330867544	14.044459502701075	62.04716998889454	16.357784177536846
16	6.811225930317917	14.851958514502982	59.66871835413256	18.66809720104655
17	6.645961563799951	14.960001505825662	58.073296064148174	20.320740866226213
18	7.216293033674026	13.801645114536864	62.225610329964056	16.75645152182506
19	7.517081709865041	15.570237355770136	62.91151391947597	14.001167014888852
20	7.753872795377115	19.84791160803358	59.68904700058351	12.7091685960058
21	7.666911363336909	20.47847610442901	59.59229770173358	12.2623148305005
22	6.053795621811885	20.077173565230485	56.31900916671372	17.550021646243906
23	6.76642761684266	19.46016149980236	54.01773109718222	19.755679786172756
24	8.52334970918742	18.8570783217573	53.258418506597394	19.361153462457885
25	7.380051574528959	19.876522295631226	51.47552092156531	21.267905208274513
26	8.144258098518645	20.073032544657142	53.60965234250005	18.17305701432417
27	9.608673555819076	18.229525476687936	56.45942740979163	15.702373557701357
28	7.440661057466072	15.162535057503717	56.56257646770945	20.83422741732076
29	10.43198373708284	13.1695747924784	54.835394432209604	21.56304703822915
30	14.998400060233028	15.672257044440679	51.05765430007341	18.271688595252886
31	12.51039960848533	21.685395373350648	47.90784346942233	17.896361548741694
32	11.149133209102716	20.59781278822444	45.825663033862256	22.427390968810588
33	13.077342970617579	18.026615468594123	44.38759952566492	24.508442035123384
34	13.611158168163081	17.263538313851715	44.95002541080807	24.175278107177142
35	16.15223897453272	17.054605003105767	41.83334274474373	24.959813277617783
36	15.244979012554822	19.302426261599564	42.21469309390705	23.237901631938563
37	10.619459032130555	20.361021702712367	47.60743124964707	21.412088015510005
38	9.984753515161781	19.158243454364072	45.83130988009863	25.025693150375517
39	12.440002258738495	19.750409396352136	41.28785739830971	26.521730946599657
40	13.364202759425527	22.3991567376287	42.70483934722458	21.531801155721197
41	12.331959267415815	23.953168821879647	44.27880362151072	19.43606828919382
42	13.617181470815215	23.164492630865663	46.22545974739774	16.992866150921376
43	13.26557117849681	19.902874244734317	47.81674101680878	19.014813559960096
44	12.781071771415665	16.569352683193102	44.61949667777213	26.0300788676191
45	17.246221318726825	14.535735125265873	42.61863082801589	25.599412727991417
46	17.405462382592653	16.362678110941705	47.3796751181132	18.852184388352438
47	10.448547819376213	21.485120560167147	49.84546464133115	18.220866979125493
48	10.341634197300808	19.67624748244772	50.614941555141456	19.36717676511002
49	16.47185047151166	15.097784553993263	49.84922920548873	18.581135769006345
50	15.064656389406517	13.250136465450712	46.04513712424944	25.64007002089333
51	12.437367063828185	12.998663579724056	43.57595949328966	30.988009863158094
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1085.0
1	1319.5
2	1554.0
3	24661.0
4	47768.0
5	28187.5
6	8607.0
7	8064.5
8	7522.0
9	7195.0
10	6868.0
11	6606.0
12	6344.0
13	5978.5
14	5613.0
15	5126.5
16	4640.0
17	4221.0
18	3802.0
19	3393.5
20	2985.0
21	2653.5
22	2322.0
23	2115.5
24	1909.0
25	1749.5
26	1511.5
27	1433.0
28	1367.5
29	1302.0
30	1351.0
31	1400.0
32	1429.0
33	1458.0
34	1530.5
35	1603.0
36	2017.0
37	2431.0
38	2865.0
39	3299.0
40	5337.0
41	7375.0
42	10051.0
43	12727.0
44	13485.5
45	14244.0
46	18735.0
47	23226.0
48	28025.5
49	32825.0
50	30861.5
51	28898.0
52	22272.5
53	15647.0
54	11185.0
55	6723.0
56	4824.5
57	2926.0
58	2300.5
59	1675.0
60	1465.5
61	1256.0
62	1077.0
63	898.0
64	737.5
65	577.0
66	490.0
67	403.0
68	331.0
69	259.0
70	213.5
71	168.0
72	142.0
73	116.0
74	97.5
75	63.5
76	48.0
77	32.5
78	17.0
79	12.0
80	7.0
81	5.5
82	4.0
83	3.0
84	2.0
85	1.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	265635.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.12365733632432
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.03400143727622	31.679198783524626
2	12.219693701451217	10.050369930666408
3	4.360681903974121	5.379815651152263
4	2.063706937622582	3.3946870778154525
5	1.0629364719123928	2.1855917620603384
6	0.6480682468161543	1.599056190757199
7	0.4045143535108127	1.1644576762982397
8	0.324997525489192	1.0692069498696688
9	0.26238238510706774	0.9711110966607487
>10	1.4073223651745106	11.245422966105657
>50	0.13019719243291986	3.664551165581827
>100	0.07056489055524663	5.585518205313544
>500	0.003975486791844881	1.1370490009646042
>1k	0.004969358489806101	3.803923455060234
>5k	9.938716979612202E-4	2.2597929282290785
>10k+	9.938716979612202E-4	14.810247159940113
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	36236	13.64127468142376	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCC	5529	2.081427522728556	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGC	3100	1.1670148888512433	No Hit
CTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGCT	1937	0.7291960773241478	Illumina PCR Primer Index 7 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGC	1834	0.6904210665010259	Illumina PCR Primer Index 7 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGC	1353	0.5093455305212039	RNA PCR Primer, Index 14 (95% over 24bp)
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCG	1083	0.40770229826641824	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	872	0.32826999454138195	No Hit
GCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGC	807	0.3038003275170817	RNA PCR Primer, Index 14 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTG	561	0.2111920492404992	Illumina PCR Primer Index 7 (95% over 21bp)
CGTTTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATG	542	0.20403937734108832	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	475	0.17881679748527113	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTC	472	0.17768742823799574	No Hit
TTCCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCT	465	0.1750522333276865	RNA PCR Primer, Index 14 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTG	458	0.17241703841737724	Illumina PCR Primer Index 7 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	389	0.1464415457300431	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	374	0.14079469949366613	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTC	365	0.13740659175183992	RNA PCR Primer, Index 14 (95% over 21bp)
CGTTTTTTCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGT	346	0.1302539198524291	No Hit
TGCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTG	334	0.1257364428633275	RNA PCR Primer, Index 14 (95% over 23bp)
TTCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTG	330	0.12423061720029363	RNA PCR Primer, Index 14 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	317	0.11933668379543359	No Hit
TTTCCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTC	311	0.1170779453008828	RNA PCR Primer, Index 14 (95% over 21bp)
CGTCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCT	277	0.10427842716509494	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.8090048374649425	0.0
2	0.0	0.0	0.0	2.497788318557419	0.0
3	0.0	0.0	0.0	3.5571366725017413	0.0
4	0.0	0.0	0.0	4.9206618103789035	0.0
5	0.0	0.0	0.0	7.473789222052817	0.0
6	0.0	0.0	0.0	9.117397933254278	0.0
7	0.0	0.0	0.0	10.9127185800064	0.0
8	0.0	0.0	0.0	13.703389990023904	0.0
9	0.0	0.0	0.0	15.082349840947165	0.0
10	0.0	0.0	0.0	18.021345078773503	0.0
11	0.0	0.0	0.0	25.356598339827208	0.0
12	0.0	0.0	0.0	31.27298736988725	0.0
13	0.0	0.0	0.0	33.21587893161669	0.0
14	0.0	0.0	0.0	33.9164643213432	0.0
15	0.0	0.0	0.0	34.75859732339488	0.0
16	0.0	0.0	0.0	36.56859977036159	0.0
17	0.0	0.0	0.0	38.89208876842284	0.0
18	0.0	0.0	0.0	41.05784252828129	0.0
19	0.0	0.0	0.0	42.44131985619365	0.0
20	0.0	0.0	0.0	43.642592278878915	0.0
21	0.0	0.0	0.0	44.97825964198995	0.0
22	0.0	0.0	0.0	46.11816966890658	0.0
23	0.0	0.0	0.0	46.88764658271688	0.0
24	0.0	0.0	0.0	47.358970015246484	0.0
25	0.0	0.0	0.0	47.699286615092134	0.0
26	0.0	0.0	0.0	47.942477459672105	0.0
27	0.0	0.0	0.0	48.12882338547255	0.0
28	0.0	0.0	0.0	48.32985111148757	0.0
29	0.0	0.0	0.0	48.4890921753534	0.0
30	0.0	0.0	0.0	48.67167353699625	0.0
31	0.0	0.0	0.0	48.81962090838933	0.0
32	3.764564157584656E-4	0.0	0.0	48.950627741073276	0.0
33	3.764564157584656E-4	0.0	0.0	49.07749355318388	0.0
34	3.764564157584656E-4	0.0	0.0	49.20774747303631	0.0
35	3.764564157584656E-4	0.0	0.0	49.33498974156267	0.0
36	3.764564157584656E-4	0.0	0.0	49.449808948369004	0.0
37	3.764564157584656E-4	0.0	0.0	49.579686411805675	0.0
38	3.764564157584656E-4	0.0	0.0	49.71784591638903	0.0
39	3.764564157584656E-4	0.0	0.0	49.87068722118697	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGGTA	20	7.023481E-4	45.000004	5
GGATGCC	20	7.023481E-4	45.000004	8
TTTGTCG	20	7.023481E-4	45.000004	1
GTTTAGG	40	6.7811925E-9	45.000004	2
TCCCTTG	35	1.2072087E-7	45.000004	1
TTCACGG	20	7.023481E-4	45.000004	2
CTTATTG	20	7.023481E-4	45.000004	2
CGGGCGA	20	7.023481E-4	45.000004	6
ACTAATC	20	7.023481E-4	45.000004	10
CCCTTGG	35	1.2072087E-7	45.000004	2
GCTAGCT	20	7.023481E-4	45.000004	4
ATTGGGC	35	1.2072087E-7	45.000004	4
ATTGGAT	20	7.023481E-4	45.000004	11
TGCTAGC	20	7.023481E-4	45.000004	3
GGCGATT	75	0.0	45.000004	8
GGGATCG	20	7.023481E-4	45.000004	8
GCTTTGT	20	7.023481E-4	45.000004	2
TGCCGGG	20	7.023481E-4	45.000004	3
CTTGTTG	45	3.8198777E-10	45.0	1
ATGGGAC	25	3.882681E-5	45.0	5
>>END_MODULE