SRR2933729.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933729.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	263881
Sequences flagged as poor quality	0
Sequence length	51
%GC	32

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35421	13.423096016765133	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCC	5566	2.1092841091249466	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGC	3135	1.1880355160091103	No Hit
CTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGCT	2012	0.7624648989506633	Illumina PCR Primer Index 7 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGC	1858	0.7041052595677597	Illumina PCR Primer Index 7 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGC	1268	0.4805196281657262	RNA PCR Primer, Index 14 (95% over 24bp)
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCG	1034	0.391843292999496	No Hit
GCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGC	823	0.3118830078709721	RNA PCR Primer, Index 14 (95% over 24bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	722	0.27360817944452237	No Hit
CGCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTG	554	0.20994311829953655	Illumina PCR Primer Index 7 (95% over 21bp)
CGTTTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATG	514	0.19478477040787326	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	487	0.18455288558100053	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTC	473	0.17924746381891837	No Hit
TCCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTG	459	0.17394204205683622	Illumina PCR Primer Index 7 (95% over 21bp)
TTCCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCT	454	0.1720472485703783	RNA PCR Primer, Index 14 (95% over 22bp)
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	390	0.14779389194371706	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGT	384	0.14552013975996755	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTC	359	0.13604617232767802	RNA PCR Primer, Index 14 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	347	0.131498667960179	No Hit
TTTCCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTC	315	0.11937198964684839	RNA PCR Primer, Index 14 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTG	300	0.11368760918747466	RNA PCR Primer, Index 14 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTG	290	0.10989802221455884	RNA PCR Primer, Index 14 (95% over 23bp)
TTCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTG	287	0.10876114612268409	RNA PCR Primer, Index 14 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	275	0.1042136417551851	No Hit
CGTCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCT	272	0.10307676566331035	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTTGCG	35	1.2071723E-7	45.000004	1
TTTGTAG	25	3.8826103E-5	45.000004	1
TGGAATG	50	2.1827873E-11	45.000004	6
GCTCTTG	25	3.8826103E-5	45.000004	1
CGTAGGG	25	3.8826103E-5	45.000004	3
CGGGAAG	25	3.8826103E-5	45.000004	6
GGGCATA	35	1.2071723E-7	45.000004	7
TCTTTAG	25	3.8826103E-5	45.000004	1
CGCTTGG	35	1.2071723E-7	45.000004	2
CGGGTCC	20	7.023398E-4	45.0	6
CCGGGAT	20	7.023398E-4	45.0	5
GGGCTAA	20	7.023398E-4	45.0	7
GGTCAAG	20	7.023398E-4	45.0	15
GGATACA	20	7.023398E-4	45.0	8
TATTGCG	20	7.023398E-4	45.0	1
GCCCTTG	20	7.023398E-4	45.0	1
GGAACAT	40	6.7811925E-9	45.0	8
CGGGAGT	20	7.023398E-4	45.0	6
GAGTTTG	20	7.023398E-4	45.0	1
CTTTAGG	20	7.023398E-4	45.0	2

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content