##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933729.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	263881
Sequences flagged as poor quality	0
Sequence length	51
%GC	32
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.876936952641532	33.0	31.0	34.0	30.0	34.0
2	32.16126966321941	34.0	31.0	34.0	30.0	34.0
3	32.141787396591646	33.0	31.0	34.0	30.0	34.0
4	35.82971490937203	37.0	35.0	37.0	35.0	37.0
5	35.93554670476465	37.0	35.0	37.0	35.0	37.0
6	35.94890499884418	37.0	35.0	37.0	35.0	37.0
7	35.88269712484036	37.0	35.0	37.0	35.0	37.0
8	35.747416449081214	37.0	35.0	37.0	35.0	37.0
9	37.549501479833715	39.0	37.0	39.0	35.0	39.0
10	37.24686885376363	39.0	37.0	39.0	34.0	39.0
11	37.27177022976266	39.0	37.0	39.0	34.0	39.0
12	37.322478693047245	39.0	37.0	39.0	34.0	39.0
13	37.28653067102216	39.0	37.0	39.0	34.0	39.0
14	38.40479231168595	40.0	38.0	41.0	34.0	41.0
15	38.38745116169789	40.0	38.0	41.0	33.0	41.0
16	38.51766894926122	40.0	38.0	41.0	34.0	41.0
17	38.55390497989624	40.0	38.0	41.0	34.0	41.0
18	38.030093110151924	39.0	38.0	40.0	34.0	41.0
19	37.42867428878927	37.0	37.0	40.0	34.0	41.0
20	36.56452719218132	36.0	35.0	40.0	33.0	41.0
21	36.454147892421204	36.0	35.0	40.0	33.0	41.0
22	36.43758739734956	36.0	35.0	40.0	33.0	41.0
23	36.330618725865065	36.0	35.0	40.0	33.0	41.0
24	36.13622049332843	36.0	35.0	40.0	33.0	41.0
25	36.03234033522686	35.0	35.0	40.0	32.0	41.0
26	36.04874924681959	35.0	35.0	40.0	33.0	41.0
27	36.001712893311755	35.0	35.0	40.0	33.0	41.0
28	35.95158423683403	35.0	35.0	40.0	33.0	41.0
29	35.7772632360799	35.0	35.0	40.0	31.0	41.0
30	35.42085258127717	35.0	35.0	40.0	31.0	41.0
31	34.87569396811441	35.0	35.0	40.0	27.0	41.0
32	34.33630689591141	35.0	35.0	40.0	22.0	41.0
33	33.52332301302481	35.0	33.0	40.0	18.0	41.0
34	32.84729101375241	35.0	33.0	40.0	13.0	41.0
35	32.38814086652696	35.0	32.0	40.0	10.0	41.0
36	32.040116567695286	35.0	31.0	40.0	10.0	41.0
37	31.847143219860467	35.0	31.0	40.0	8.0	41.0
38	31.715364122464294	35.0	31.0	40.0	8.0	41.0
39	31.62207207036505	35.0	30.0	40.0	8.0	41.0
40	31.38116802649679	35.0	30.0	40.0	8.0	41.0
41	31.17645453821988	35.0	29.0	40.0	7.0	41.0
42	30.933204740015384	35.0	27.0	40.0	7.0	41.0
43	30.76501150139646	35.0	26.0	40.0	7.0	41.0
44	30.617475301366905	35.0	24.0	40.0	7.0	41.0
45	30.548607137308103	35.0	24.0	40.0	7.0	41.0
46	30.155877080956948	35.0	23.0	39.0	7.0	41.0
47	30.01806875068686	35.0	23.0	39.0	7.0	41.0
48	29.91775459392681	35.0	23.0	39.0	7.0	41.0
49	29.923624664147855	35.0	23.0	39.0	7.0	41.0
50	29.688086675433244	35.0	22.0	39.0	7.0	41.0
51	28.82978691152451	35.0	20.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	3.0
10	6.0
11	7.0
12	6.0
13	11.0
14	17.0
15	26.0
16	36.0
17	80.0
18	166.0
19	377.0
20	670.0
21	1040.0
22	1552.0
23	2406.0
24	4120.0
25	7578.0
26	10983.0
27	11292.0
28	9709.0
29	8650.0
30	8212.0
31	8636.0
32	8933.0
33	10333.0
34	13706.0
35	23782.0
36	32164.0
37	25586.0
38	42398.0
39	31391.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	15.014722545389777	3.4985466933958866	31.65934644783065	49.82738431338368
2	52.92688749853154	3.811187618661442	29.948347929559155	13.313576953247866
3	11.96865253656004	4.17233525718032	69.85307771306005	14.005934493199588
4	10.580905787078267	3.638003493999189	70.48404394405054	15.297046774872006
5	9.116988339440885	4.403121103830894	70.17140301878499	16.30848753794324
6	11.76742546829821	5.297842588136319	73.10909084018932	9.825641103376142
7	42.55895649933114	2.587908943804215	49.51739609899917	5.335738457865477
8	40.525085170967216	3.0172691478355773	49.34686468521796	7.110780995979248
9	36.54336613852456	4.595632122055017	51.999196607561736	6.861805131858678
10	18.241555852827602	15.833273331539596	54.87511416130756	11.050056654325244
11	11.353602570855802	13.21391081586018	57.48689750304115	17.945589110242874
12	10.579010993591808	11.593104467544082	61.89797673951516	15.92990779934895
13	14.872991992602728	12.253250518226018	62.168553249381354	10.705204239789905
14	12.113793717622716	14.597110060974455	60.20478928001637	13.084306941386458
15	7.550373084837483	13.982060095270215	61.957473254989935	16.510093564902363
16	6.966776691008447	14.824864238046695	59.469230448573406	18.73912862237145
17	6.834899064350976	14.952194360336668	57.99015465304437	20.222751922267992
18	7.451843823541672	13.711862544101319	62.06888711199367	16.767406520363345
19	7.641702130884755	15.533517001981952	62.85825807845203	13.966522788681262
20	7.872109018838038	19.878278466429943	59.492725887805484	12.75688662692653
21	7.745157855245356	20.597163115192075	59.37259598076405	12.285083048798512
22	6.192185113744452	19.983628984277004	56.16584748428268	17.658338417695855
23	6.972461071467821	19.48984580170607	53.77272331088635	19.76496981593976
24	8.481853562780191	18.860016446807464	53.143651873382325	19.514478117030023
25	7.452980699633546	19.90366869914848	51.49139195319103	21.15195864802695
26	8.243488542183787	20.037062160595116	53.48926220531224	18.230187091908853
27	9.67633137664326	18.228292298422392	56.420128770165334	15.675247554769006
28	7.448054236568756	15.287572807439718	56.46408797905116	20.800284976940365
29	10.533535949916818	13.209742270189972	54.60870619711158	21.648015582781632
30	15.08445094569143	15.843126257669176	50.89566888104865	18.17675391559074
31	12.626903793755517	21.740102546223486	47.68778350847541	17.94521015154558
32	11.210735141976876	20.760115355027455	45.53908769483214	22.490061808163528
33	13.228311246357261	17.929293886259337	44.27071293499721	24.571681932386188
34	13.717546924560692	17.231251965848244	44.90357395947416	24.14762715011691
35	16.254296444230544	17.080047445628903	41.70023609126841	24.96542001887214
36	15.233760672424312	19.313630007465488	42.236462647936	23.2161466721742
37	10.60553810240222	20.327723481417763	47.58660153629856	21.480136879881464
38	10.101144076307124	19.107097517441574	45.797537526385	24.9942208798663
39	12.589386882723652	19.7767175355558	41.224263967470186	26.40963161425036
40	13.3795157665766	22.562063960648928	42.567672549368844	21.490747723405626
41	12.327526422895168	24.00892826690819	44.19643703032806	19.46710827986858
42	13.785759490073177	23.13770222183484	46.15186390835263	16.92467437973935
43	13.196099757087476	19.985902736460755	47.83860906999746	18.97938843645431
44	12.781518942250484	16.765511726876888	44.50756212080446	25.945407210068172
45	17.20472485703783	14.659638246027566	42.69689746514527	25.43873943178933
46	17.390414618710707	16.515398986664444	47.467229546651716	18.62695684797314
47	10.311845112001244	21.615425134814554	49.91871335943095	18.154016393753245
48	10.469491930074541	19.6956203743354	50.547026879540404	19.28786081604966
49	16.530178375858814	15.079524482626638	49.8986285484745	18.491668593040046
50	15.153421428598495	13.25218564428663	45.91842535082101	25.67596757629386
51	12.523069110697623	13.228690205054553	43.477931340263225	30.770309343984596
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	941.0
1	1250.5
2	1560.0
3	24188.0
4	46816.0
5	27906.0
6	8996.0
7	8228.5
8	7461.0
9	7219.0
10	6977.0
11	6602.5
12	6228.0
13	5948.0
14	5668.0
15	5125.5
16	4583.0
17	4064.0
18	3545.0
19	3217.0
20	2889.0
21	2605.5
22	2322.0
23	2069.5
24	1817.0
25	1692.5
26	1497.0
27	1426.0
28	1352.0
29	1278.0
30	1294.5
31	1311.0
32	1400.0
33	1489.0
34	1614.5
35	1740.0
36	2106.0
37	2472.0
38	2865.0
39	3258.0
40	5371.0
41	7484.0
42	10037.0
43	12590.0
44	13306.0
45	14022.0
46	18542.5
47	23063.0
48	27748.0
49	32433.0
50	30642.5
51	28852.0
52	22321.0
53	15790.0
54	11266.5
55	6743.0
56	4870.5
57	2998.0
58	2362.0
59	1726.0
60	1501.0
61	1276.0
62	1069.0
63	862.0
64	712.0
65	562.0
66	478.5
67	395.0
68	330.5
69	266.0
70	220.5
71	175.0
72	155.0
73	135.0
74	107.0
75	62.5
76	46.0
77	31.5
78	17.0
79	14.0
80	11.0
81	10.5
82	10.0
83	5.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	263881.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.480514294541315
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.09397908717297	31.978979015485475
2	12.288584033257356	10.194735713024079
3	4.381663223695921	5.452609319531539
4	1.9622936305742338	3.2558779597248755
5	1.0383429315854316	2.1535499408132712
6	0.6482619940665627	1.6134144546891545
7	0.4434137034644949	1.2875119925468137
8	0.27439571843602967	0.910566041675733
9	0.24755194352024615	0.9241723738649751
>10	1.410746778697989	11.178724551286018
>50	0.13222643908174614	3.757720765866318
>100	0.06760449516961457	5.473686098040347
>500	0.0039767350099773275	1.0775816902116648
>1k	0.004970918762471659	3.8381373099119647
>5k	9.941837524943319E-4	2.295376841836252
>10k+	9.941837524943319E-4	14.607355931491533
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35421	13.423096016765133	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCC	5566	2.1092841091249466	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGC	3135	1.1880355160091103	No Hit
CTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGCT	2012	0.7624648989506633	Illumina PCR Primer Index 7 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGC	1858	0.7041052595677597	Illumina PCR Primer Index 7 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGC	1268	0.4805196281657262	RNA PCR Primer, Index 14 (95% over 24bp)
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCG	1034	0.391843292999496	No Hit
GCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGC	823	0.3118830078709721	RNA PCR Primer, Index 14 (95% over 24bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	722	0.27360817944452237	No Hit
CGCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTG	554	0.20994311829953655	Illumina PCR Primer Index 7 (95% over 21bp)
CGTTTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATG	514	0.19478477040787326	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	487	0.18455288558100053	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTC	473	0.17924746381891837	No Hit
TCCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTG	459	0.17394204205683622	Illumina PCR Primer Index 7 (95% over 21bp)
TTCCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCT	454	0.1720472485703783	RNA PCR Primer, Index 14 (95% over 22bp)
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	390	0.14779389194371706	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGT	384	0.14552013975996755	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTC	359	0.13604617232767802	RNA PCR Primer, Index 14 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	347	0.131498667960179	No Hit
TTTCCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTC	315	0.11937198964684839	RNA PCR Primer, Index 14 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTG	300	0.11368760918747466	RNA PCR Primer, Index 14 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTG	290	0.10989802221455884	RNA PCR Primer, Index 14 (95% over 23bp)
TTCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTG	287	0.10876114612268409	RNA PCR Primer, Index 14 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	275	0.1042136417551851	No Hit
CGTCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCT	272	0.10307676566331035	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.8310564231604397	0.0
2	0.0	0.0	0.0	2.4738423759194483	0.0
3	0.0	0.0	0.0	3.5114312891038004	0.0
4	0.0	0.0	0.0	4.841576316597254	0.0
5	0.0	0.0	0.0	7.3373982969596145	0.0
6	0.0	0.0	0.0	8.920308775546554	0.0
7	0.0	0.0	0.0	10.69951985933053	0.0
8	0.0	0.0	0.0	13.504572136682823	0.0
9	0.0	0.0	0.0	14.882087001337725	0.0
10	0.0	0.0	0.0	17.845165055460605	0.0
11	0.0	0.0	0.0	25.161720624069183	0.0
12	0.0	0.0	0.0	31.049601903888494	0.0
13	0.0	0.0	0.0	32.91559452935225	0.0
14	0.0	0.0	0.0	33.594309556201466	0.0
15	0.0	0.0	0.0	34.46212497299919	0.0
16	0.0	0.0	0.0	36.29704298528503	0.0
17	0.0	0.0	0.0	38.63862877584972	0.0
18	0.0	0.0	0.0	40.761555398077164	0.0
19	0.0	0.0	0.0	42.14702839537519	0.0
20	0.0	0.0	0.0	43.39001292249158	0.0
21	0.0	0.0	0.0	44.69666251075295	0.0
22	0.0	0.0	0.0	45.81004316339562	0.0
23	0.0	0.0	0.0	46.59486662548649	0.0
24	0.0	0.0	0.0	47.07728104713867	0.0
25	0.0	0.0	0.0	47.398638022441936	0.0
26	0.0	0.0	0.0	47.62601324081688	0.0
27	0.0	0.0	0.0	47.818903217738296	0.0
28	0.0	0.0	0.0	48.00610881420034	0.0
29	0.0	0.0	0.0	48.19558816284613	0.0
30	0.0	0.0	0.0	48.37559354405963	0.0
31	0.0	0.0	0.0	48.523766394700644	0.0
32	3.789586972915822E-4	0.0	0.0	48.661707360514775	0.0
33	3.789586972915822E-4	0.0	0.0	48.80381687199912	0.0
34	3.789586972915822E-4	0.0	0.0	48.92659948992159	0.0
35	3.789586972915822E-4	0.0	0.0	49.062645662249274	0.0
36	3.789586972915822E-4	0.0	0.0	49.17784910622591	0.0
37	3.789586972915822E-4	0.0	0.0	49.30404235242401	0.0
38	3.789586972915822E-4	0.0	0.0	49.44312019433002	0.0
39	3.789586972915822E-4	0.0	0.0	49.5924299210629	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTTGCG	35	1.2071723E-7	45.000004	1
TTTGTAG	25	3.8826103E-5	45.000004	1
TGGAATG	50	2.1827873E-11	45.000004	6
GCTCTTG	25	3.8826103E-5	45.000004	1
CGTAGGG	25	3.8826103E-5	45.000004	3
CGGGAAG	25	3.8826103E-5	45.000004	6
GGGCATA	35	1.2071723E-7	45.000004	7
TCTTTAG	25	3.8826103E-5	45.000004	1
CGCTTGG	35	1.2071723E-7	45.000004	2
CGGGTCC	20	7.023398E-4	45.0	6
CCGGGAT	20	7.023398E-4	45.0	5
GGGCTAA	20	7.023398E-4	45.0	7
GGTCAAG	20	7.023398E-4	45.0	15
GGATACA	20	7.023398E-4	45.0	8
TATTGCG	20	7.023398E-4	45.0	1
GCCCTTG	20	7.023398E-4	45.0	1
GGAACAT	40	6.7811925E-9	45.0	8
CGGGAGT	20	7.023398E-4	45.0	6
GAGTTTG	20	7.023398E-4	45.0	1
CTTTAGG	20	7.023398E-4	45.0	2
>>END_MODULE